Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3628 and BT3629 overlap by 14 nucleotides BT3629 and BT3630 are separated by 57 nucleotides BT3630 and BT3631 are separated by 32 nucleotides
BT3628: BT3628 - conserved hypothetical protein (NCBI ptt file), at 4,700,594 to 4,701,160
BT3628
BT3629: BT3629 - hypothetical protein (NCBI ptt file), at 4,701,147 to 4,701,734
BT3629
BT3630: BT3630 - hypothetical protein (NCBI ptt file), at 4,701,792 to 4,703,417
BT3630
BT3631: BT3631 - hypothetical protein (NCBI ptt file), at 4,703,450 to 4,704,958
BT3631
Position (kb)
4701
4702
4703
4704 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4700.912 kb on - strand, within BT3628 at 4700.914 kb on + strand, within BT3628 at 4700.914 kb on - strand, within BT3628 at 4701.120 kb on - strand at 4701.127 kb on + strand at 4701.181 kb on + strand at 4701.181 kb on + strand at 4701.370 kb on + strand, within BT3629 at 4701.530 kb on + strand, within BT3629 at 4701.530 kb on + strand, within BT3629 at 4701.717 kb on + strand at 4701.752 kb on - strand at 4701.760 kb on + strand at 4701.831 kb on - strand at 4701.842 kb on + strand at 4701.844 kb on + strand at 4701.845 kb on - strand at 4701.915 kb on - strand at 4701.938 kb on + strand at 4701.943 kb on + strand at 4701.956 kb on - strand, within BT3630 at 4701.956 kb on - strand, within BT3630 at 4701.976 kb on + strand, within BT3630 at 4701.983 kb on + strand, within BT3630 at 4702.029 kb on + strand, within BT3630 at 4702.080 kb on + strand, within BT3630 at 4702.107 kb on - strand, within BT3630 at 4702.291 kb on + strand, within BT3630 at 4702.348 kb on + strand, within BT3630 at 4702.364 kb on + strand, within BT3630 at 4702.412 kb on + strand, within BT3630 at 4702.423 kb on + strand, within BT3630 at 4702.471 kb on + strand, within BT3630 at 4702.536 kb on - strand at 4702.540 kb on + strand, within BT3630 at 4702.544 kb on + strand, within BT3630 at 4702.564 kb on + strand, within BT3630 at 4702.661 kb on + strand, within BT3630 at 4702.694 kb on - strand, within BT3630 at 4702.694 kb on - strand, within BT3630 at 4702.742 kb on + strand, within BT3630 at 4702.743 kb on - strand, within BT3630 at 4702.761 kb on + strand, within BT3630 at 4702.761 kb on + strand, within BT3630 at 4702.795 kb on - strand, within BT3630 at 4702.896 kb on - strand, within BT3630 at 4702.928 kb on + strand, within BT3630 at 4702.928 kb on + strand, within BT3630 at 4702.928 kb on + strand, within BT3630 at 4702.928 kb on + strand, within BT3630 at 4702.929 kb on - strand, within BT3630 at 4702.937 kb on + strand, within BT3630 at 4702.964 kb on + strand, within BT3630 at 4703.025 kb on + strand, within BT3630 at 4703.106 kb on + strand, within BT3630 at 4703.129 kb on - strand, within BT3630 at 4703.132 kb on - strand, within BT3630 at 4703.210 kb on + strand, within BT3630 at 4703.211 kb on - strand, within BT3630 at 4703.230 kb on - strand, within BT3630 at 4703.230 kb on - strand, within BT3630 at 4703.340 kb on + strand at 4703.340 kb on + strand at 4703.341 kb on - strand at 4703.397 kb on + strand at 4703.438 kb on - strand at 4703.451 kb on + strand at 4703.459 kb on - strand at 4703.503 kb on + strand at 4703.527 kb on + strand at 4703.527 kb on + strand at 4703.528 kb on - strand at 4703.613 kb on + strand, within BT3631 at 4703.684 kb on - strand, within BT3631 at 4703.814 kb on + strand, within BT3631 at 4703.814 kb on + strand, within BT3631 at 4703.815 kb on - strand, within BT3631 at 4703.815 kb on - strand, within BT3631 at 4703.825 kb on - strand, within BT3631 at 4703.972 kb on + strand, within BT3631 at 4704.082 kb on + strand, within BT3631 at 4704.276 kb on + strand, within BT3631 at 4704.286 kb on - strand, within BT3631 at 4704.286 kb on - strand, within BT3631 at 4704.316 kb on - strand, within BT3631 at 4704.397 kb on - strand, within BT3631
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 4,700,912 - BT3628 0.56 -3.0 4,700,914 + BT3628 0.56 -3.0 4,700,914 - BT3628 0.56 -3.9 4,701,120 - +1.0 4,701,127 + +0.0 4,701,181 + -1.5 4,701,181 + -2.8 4,701,370 + BT3629 0.38 +0.0 4,701,530 + BT3629 0.65 -0.8 4,701,530 + BT3629 0.65 -1.7 4,701,717 + -2.9 4,701,752 - -1.5 4,701,760 + -1.0 4,701,831 - -1.0 4,701,842 + +1.0 4,701,844 + -0.3 4,701,845 - -3.3 4,701,915 - -0.6 4,701,938 + -1.8 4,701,943 + -1.0 4,701,956 - BT3630 0.10 -2.5 4,701,956 - BT3630 0.10 -4.0 4,701,976 + BT3630 0.11 -1.5 4,701,983 + BT3630 0.12 +0.0 4,702,029 + BT3630 0.15 -0.5 4,702,080 + BT3630 0.18 -0.9 4,702,107 - BT3630 0.19 +0.1 4,702,291 + BT3630 0.31 -0.1 4,702,348 + BT3630 0.34 -0.8 4,702,364 + BT3630 0.35 -2.3 4,702,412 + BT3630 0.38 -2.8 4,702,423 + BT3630 0.39 -0.9 4,702,471 + BT3630 0.42 -1.9 4,702,536 - +0.1 4,702,540 + BT3630 0.46 +2.1 4,702,544 + BT3630 0.46 +0.1 4,702,564 + BT3630 0.47 -1.5 4,702,661 + BT3630 0.53 -1.9 4,702,694 - BT3630 0.55 +1.1 4,702,694 - BT3630 0.55 -3.0 4,702,742 + BT3630 0.58 +0.1 4,702,743 - BT3630 0.58 -0.9 4,702,761 + BT3630 0.60 -0.9 4,702,761 + BT3630 0.60 -1.0 4,702,795 - BT3630 0.62 +0.4 4,702,896 - BT3630 0.68 +0.1 4,702,928 + BT3630 0.70 +0.1 4,702,928 + BT3630 0.70 -0.9 4,702,928 + BT3630 0.70 -0.9 4,702,928 + BT3630 0.70 -2.4 4,702,929 - BT3630 0.70 -1.3 4,702,937 + BT3630 0.70 -2.3 4,702,964 + BT3630 0.72 -1.9 4,703,025 + BT3630 0.76 -0.2 4,703,106 + BT3630 0.81 -3.5 4,703,129 - BT3630 0.82 -2.5 4,703,132 - BT3630 0.82 -3.5 4,703,210 + BT3630 0.87 +0.1 4,703,211 - BT3630 0.87 -1.8 4,703,230 - BT3630 0.88 -2.8 4,703,230 - BT3630 0.88 -0.9 4,703,340 + -3.9 4,703,340 + -0.9 4,703,341 - -1.9 4,703,397 + -1.5 4,703,438 - +0.2 4,703,451 + +0.1 4,703,459 - +0.5 4,703,503 + -1.9 4,703,527 + +0.1 4,703,527 + -2.3 4,703,528 - -2.5 4,703,613 + BT3631 0.11 +0.1 4,703,684 - BT3631 0.16 +0.5 4,703,814 + BT3631 0.24 -0.9 4,703,814 + BT3631 0.24 -2.6 4,703,815 - BT3631 0.24 +1.1 4,703,815 - BT3631 0.24 -1.9 4,703,825 - BT3631 0.25 +0.1 4,703,972 + BT3631 0.35 -2.6 4,704,082 + BT3631 0.42 -1.0 4,704,276 + BT3631 0.55 -0.1 4,704,286 - BT3631 0.55 -1.4 4,704,286 - BT3631 0.55 -0.8 4,704,316 - BT3631 0.57 -2.3 4,704,397 - BT3631 0.63 -2.3
Or see this region's nucleotide sequence