Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3599 and BT3600 are separated by 6 nucleotides BT3600 and BT3601 are separated by 3 nucleotides
BT3599: BT3599 - beta-mannosidase precursor (NCBI ptt file), at 4,660,728 to 4,663,490
BT3599
BT3600: BT3600 - ROK family transcriptional repressor (NCBI ptt file), at 4,663,497 to 4,664,393
BT3600
BT3601: BT3601 - glucosamine--fructose-6-phosphate aminotransferase (NCBI ptt file), at 4,664,397 to 4,665,446
BT3601
Position (kb)
4663
4664
4665 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4662.501 kb on + strand, within BT3599 at 4662.504 kb on + strand, within BT3599 at 4662.505 kb on - strand, within BT3599 at 4662.600 kb on + strand, within BT3599 at 4662.600 kb on + strand, within BT3599 at 4662.636 kb on - strand, within BT3599 at 4662.643 kb on - strand, within BT3599 at 4662.666 kb on + strand, within BT3599 at 4662.683 kb on + strand, within BT3599 at 4662.683 kb on + strand, within BT3599 at 4662.719 kb on + strand, within BT3599 at 4662.719 kb on + strand, within BT3599 at 4662.726 kb on - strand, within BT3599 at 4662.728 kb on + strand, within BT3599 at 4662.728 kb on + strand, within BT3599 at 4662.728 kb on + strand, within BT3599 at 4662.729 kb on - strand, within BT3599 at 4662.730 kb on + strand, within BT3599 at 4662.731 kb on - strand, within BT3599 at 4662.785 kb on + strand, within BT3599 at 4662.786 kb on - strand, within BT3599 at 4662.840 kb on + strand, within BT3599 at 4662.895 kb on - strand, within BT3599 at 4662.896 kb on + strand, within BT3599 at 4662.896 kb on + strand, within BT3599 at 4662.897 kb on - strand, within BT3599 at 4662.897 kb on - strand, within BT3599 at 4662.897 kb on - strand, within BT3599 at 4662.989 kb on + strand, within BT3599 at 4663.018 kb on - strand, within BT3599 at 4663.020 kb on + strand, within BT3599 at 4663.020 kb on + strand, within BT3599 at 4663.020 kb on + strand, within BT3599 at 4663.020 kb on + strand, within BT3599 at 4663.059 kb on + strand, within BT3599 at 4663.077 kb on - strand, within BT3599 at 4663.112 kb on + strand, within BT3599 at 4663.114 kb on + strand, within BT3599 at 4663.192 kb on + strand, within BT3599 at 4663.193 kb on - strand, within BT3599 at 4663.193 kb on - strand, within BT3599 at 4663.284 kb on - strand at 4663.294 kb on - strand at 4663.294 kb on - strand at 4663.317 kb on + strand at 4663.340 kb on + strand at 4663.358 kb on + strand at 4663.358 kb on + strand at 4663.359 kb on - strand at 4663.359 kb on - strand at 4663.431 kb on - strand at 4663.459 kb on - strand at 4663.468 kb on + strand at 4663.474 kb on - strand at 4663.475 kb on + strand at 4663.476 kb on - strand at 4663.541 kb on + strand at 4663.541 kb on + strand at 4663.563 kb on + strand at 4663.597 kb on + strand, within BT3600 at 4663.608 kb on + strand, within BT3600 at 4663.632 kb on + strand, within BT3600 at 4663.632 kb on + strand, within BT3600 at 4663.634 kb on + strand, within BT3600 at 4663.635 kb on - strand, within BT3600 at 4663.664 kb on + strand, within BT3600 at 4663.689 kb on - strand, within BT3600 at 4663.824 kb on + strand, within BT3600 at 4663.825 kb on - strand, within BT3600 at 4663.848 kb on + strand, within BT3600 at 4663.852 kb on + strand, within BT3600 at 4663.860 kb on + strand, within BT3600 at 4663.860 kb on + strand, within BT3600 at 4663.876 kb on + strand, within BT3600 at 4663.935 kb on + strand, within BT3600 at 4663.942 kb on - strand, within BT3600 at 4663.943 kb on + strand, within BT3600 at 4663.943 kb on + strand, within BT3600 at 4663.944 kb on - strand, within BT3600 at 4663.953 kb on + strand, within BT3600 at 4663.977 kb on + strand, within BT3600 at 4663.978 kb on - strand, within BT3600 at 4664.099 kb on - strand, within BT3600 at 4664.107 kb on - strand, within BT3600 at 4664.154 kb on + strand, within BT3600 at 4664.154 kb on + strand, within BT3600 at 4664.154 kb on + strand, within BT3600 at 4664.283 kb on - strand, within BT3600 at 4664.351 kb on + strand at 4664.412 kb on + strand at 4664.429 kb on + strand at 4664.429 kb on + strand at 4664.430 kb on - strand at 4664.468 kb on + strand at 4664.496 kb on - strand at 4664.538 kb on - strand, within BT3601 at 4664.548 kb on - strand, within BT3601 at 4664.586 kb on - strand, within BT3601 at 4664.605 kb on + strand, within BT3601 at 4664.609 kb on + strand, within BT3601 at 4664.615 kb on + strand, within BT3601 at 4664.631 kb on + strand, within BT3601 at 4664.670 kb on + strand, within BT3601 at 4664.694 kb on + strand, within BT3601 at 4664.815 kb on - strand, within BT3601 at 4664.874 kb on - strand, within BT3601 at 4664.965 kb on + strand, within BT3601 at 4664.966 kb on - strand, within BT3601 at 4664.970 kb on + strand, within BT3601 at 4664.985 kb on + strand, within BT3601 at 4665.033 kb on + strand, within BT3601 at 4665.033 kb on + strand, within BT3601 at 4665.038 kb on + strand, within BT3601 at 4665.039 kb on - strand, within BT3601 at 4665.039 kb on - strand, within BT3601 at 4665.118 kb on - strand, within BT3601 at 4665.137 kb on - strand, within BT3601 at 4665.163 kb on - strand, within BT3601 at 4665.163 kb on - strand, within BT3601 at 4665.164 kb on + strand, within BT3601 at 4665.199 kb on + strand, within BT3601 at 4665.230 kb on - strand, within BT3601 at 4665.243 kb on - strand, within BT3601 at 4665.317 kb on + strand, within BT3601 at 4665.317 kb on + strand, within BT3601 at 4665.372 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 4,662,501 + BT3599 0.64 -2.2 4,662,504 + BT3599 0.64 +0.0 4,662,505 - BT3599 0.64 +0.0 4,662,600 + BT3599 0.68 +0.3 4,662,600 + BT3599 0.68 -1.8 4,662,636 - BT3599 0.69 +0.0 4,662,643 - BT3599 0.69 -0.8 4,662,666 + BT3599 0.70 -1.0 4,662,683 + BT3599 0.71 -2.3 4,662,683 + BT3599 0.71 -3.4 4,662,719 + BT3599 0.72 -2.9 4,662,719 + BT3599 0.72 +0.0 4,662,726 - BT3599 0.72 -0.2 4,662,728 + BT3599 0.72 -2.8 4,662,728 + BT3599 0.72 -2.1 4,662,728 + BT3599 0.72 -3.1 4,662,729 - BT3599 0.72 -1.0 4,662,730 + BT3599 0.72 -1.8 4,662,731 - BT3599 0.72 -1.0 4,662,785 + BT3599 0.74 -0.6 4,662,786 - BT3599 0.74 -1.0 4,662,840 + BT3599 0.76 -3.4 4,662,895 - BT3599 0.78 -2.8 4,662,896 + BT3599 0.78 -2.3 4,662,896 + BT3599 0.78 +1.8 4,662,897 - BT3599 0.79 -3.1 4,662,897 - BT3599 0.79 -2.6 4,662,897 - BT3599 0.79 +0.0 4,662,989 + BT3599 0.82 -3.6 4,663,018 - BT3599 0.83 -0.6 4,663,020 + BT3599 0.83 +0.0 4,663,020 + BT3599 0.83 -1.6 4,663,020 + BT3599 0.83 -2.7 4,663,020 + BT3599 0.83 -1.6 4,663,059 + BT3599 0.84 -2.0 4,663,077 - BT3599 0.85 -1.0 4,663,112 + BT3599 0.86 -1.8 4,663,114 + BT3599 0.86 +2.8 4,663,192 + BT3599 0.89 -1.0 4,663,193 - BT3599 0.89 -3.3 4,663,193 - BT3599 0.89 +0.0 4,663,284 - -1.0 4,663,294 - -0.6 4,663,294 - -1.0 4,663,317 + -1.3 4,663,340 + -1.7 4,663,358 + -1.0 4,663,358 + -1.2 4,663,359 - +0.0 4,663,359 - -2.6 4,663,431 - -0.6 4,663,459 - -1.6 4,663,468 + -2.0 4,663,474 - -2.0 4,663,475 + -2.4 4,663,476 - -3.2 4,663,541 + -3.4 4,663,541 + -2.8 4,663,563 + +0.0 4,663,597 + BT3600 0.11 +0.5 4,663,608 + BT3600 0.12 -1.0 4,663,632 + BT3600 0.15 -3.3 4,663,632 + BT3600 0.15 -1.0 4,663,634 + BT3600 0.15 +0.2 4,663,635 - BT3600 0.15 +2.6 4,663,664 + BT3600 0.19 -2.1 4,663,689 - BT3600 0.21 -1.6 4,663,824 + BT3600 0.36 -0.3 4,663,825 - BT3600 0.37 +0.0 4,663,848 + BT3600 0.39 -0.3 4,663,852 + BT3600 0.40 -3.0 4,663,860 + BT3600 0.40 -1.5 4,663,860 + BT3600 0.40 -3.1 4,663,876 + BT3600 0.42 -1.6 4,663,935 + BT3600 0.49 -0.2 4,663,942 - BT3600 0.50 -2.6 4,663,943 + BT3600 0.50 -0.7 4,663,943 + BT3600 0.50 +1.6 4,663,944 - BT3600 0.50 +0.0 4,663,953 + BT3600 0.51 +1.6 4,663,977 + BT3600 0.54 -1.0 4,663,978 - BT3600 0.54 +0.0 4,664,099 - BT3600 0.67 -1.0 4,664,107 - BT3600 0.68 +2.0 4,664,154 + BT3600 0.73 -1.0 4,664,154 + BT3600 0.73 -2.0 4,664,154 + BT3600 0.73 -1.8 4,664,283 - BT3600 0.88 -1.1 4,664,351 + -2.4 4,664,412 + -1.6 4,664,429 + -1.6 4,664,429 + -1.6 4,664,430 - +0.0 4,664,468 + -2.3 4,664,496 - +0.0 4,664,538 - BT3601 0.13 -1.1 4,664,548 - BT3601 0.14 -3.7 4,664,586 - BT3601 0.18 -2.0 4,664,605 + BT3601 0.20 +0.2 4,664,609 + BT3601 0.20 +1.0 4,664,615 + BT3601 0.21 -1.0 4,664,631 + BT3601 0.22 -2.3 4,664,670 + BT3601 0.26 -2.1 4,664,694 + BT3601 0.28 -0.6 4,664,815 - BT3601 0.40 -2.6 4,664,874 - BT3601 0.45 -1.6 4,664,965 + BT3601 0.54 -2.9 4,664,966 - BT3601 0.54 -1.6 4,664,970 + BT3601 0.55 -1.0 4,664,985 + BT3601 0.56 +1.0 4,665,033 + BT3601 0.61 -2.0 4,665,033 + BT3601 0.61 +0.1 4,665,038 + BT3601 0.61 -4.4 4,665,039 - BT3601 0.61 -1.0 4,665,039 - BT3601 0.61 -1.0 4,665,118 - BT3601 0.69 -2.0 4,665,137 - BT3601 0.70 -1.0 4,665,163 - BT3601 0.73 +2.2 4,665,163 - BT3601 0.73 -2.7 4,665,164 + BT3601 0.73 -1.0 4,665,199 + BT3601 0.76 -2.3 4,665,230 - BT3601 0.79 -3.0 4,665,243 - BT3601 0.81 +0.6 4,665,317 + BT3601 0.88 -2.9 4,665,317 + BT3601 0.88 +0.0 4,665,372 + -2.0
Or see this region's nucleotide sequence