Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3460 and BT3461 are separated by 124 nucleotides BT3461 and BT3462 are separated by 18 nucleotides BT3462 and BT3463 overlap by 4 nucleotides BT3463 and BT3464 overlap by 4 nucleotides
BT3460: BT3460 - dGTP triphosphohydrolase (NCBI ptt file), at 4,455,570 to 4,456,922
BT3460
BT3461: BT3461 - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI ptt file), at 4,457,047 to 4,457,481
BT3461
BT3462: BT3462 - TPR domain protein (NCBI ptt file), at 4,457,500 to 4,459,254
BT3462
BT3463: BT3463 - hypothetical protein (NCBI ptt file), at 4,459,251 to 4,459,832
BT3463
BT3464: BT3464 - putative peptidase (NCBI ptt file), at 4,459,829 to 4,461,136
BT3464
Position (kb)
4457
4458
4459
4460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4456.541 kb on + strand at 4456.584 kb on + strand, within BT3460 at 4456.585 kb on - strand, within BT3460 at 4456.903 kb on - strand at 4456.903 kb on - strand at 4456.930 kb on - strand at 4457.514 kb on + strand at 4457.517 kb on + strand at 4457.721 kb on + strand, within BT3462 at 4457.769 kb on + strand, within BT3462 at 4457.845 kb on + strand, within BT3462 at 4458.073 kb on + strand, within BT3462 at 4458.173 kb on + strand, within BT3462 at 4458.255 kb on + strand, within BT3462 at 4458.510 kb on + strand, within BT3462 at 4458.539 kb on - strand, within BT3462 at 4458.731 kb on + strand, within BT3462 at 4458.820 kb on + strand, within BT3462 at 4458.820 kb on + strand, within BT3462 at 4458.820 kb on + strand, within BT3462 at 4459.015 kb on + strand, within BT3462 at 4459.045 kb on + strand, within BT3462 at 4459.131 kb on + strand at 4459.819 kb on + strand at 4459.828 kb on + strand at 4459.829 kb on - strand at 4459.993 kb on - strand, within BT3464 at 4459.993 kb on - strand, within BT3464 at 4459.993 kb on - strand, within BT3464 at 4460.097 kb on + strand, within BT3464 at 4460.183 kb on + strand, within BT3464 at 4460.184 kb on - strand, within BT3464 at 4460.186 kb on - strand, within BT3464 at 4460.186 kb on - strand, within BT3464 at 4460.249 kb on + strand, within BT3464
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 4,456,541 + -0.6 4,456,584 + BT3460 0.75 -1.1 4,456,585 - BT3460 0.75 -3.5 4,456,903 - -2.9 4,456,903 - -1.6 4,456,930 - -2.9 4,457,514 + -1.6 4,457,517 + -0.1 4,457,721 + BT3462 0.13 -4.5 4,457,769 + BT3462 0.15 -2.9 4,457,845 + BT3462 0.20 -2.9 4,458,073 + BT3462 0.33 -1.1 4,458,173 + BT3462 0.38 -1.1 4,458,255 + BT3462 0.43 -0.1 4,458,510 + BT3462 0.58 -1.5 4,458,539 - BT3462 0.59 -1.6 4,458,731 + BT3462 0.70 -1.1 4,458,820 + BT3462 0.75 -0.1 4,458,820 + BT3462 0.75 -2.4 4,458,820 + BT3462 0.75 -2.4 4,459,015 + BT3462 0.86 -0.1 4,459,045 + BT3462 0.88 -1.1 4,459,131 + -1.6 4,459,819 + -2.1 4,459,828 + -1.1 4,459,829 - -0.1 4,459,993 - BT3464 0.13 -2.4 4,459,993 - BT3464 0.13 -0.5 4,459,993 - BT3464 0.13 -0.1 4,460,097 + BT3464 0.20 +1.9 4,460,183 + BT3464 0.27 +0.9 4,460,184 - BT3464 0.27 +1.9 4,460,186 - BT3464 0.27 -0.1 4,460,186 - BT3464 0.27 -1.4 4,460,249 + BT3464 0.32 -0.1
Or see this region's nucleotide sequence