Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2107 and BT2108 are separated by 106 nucleotides BT2108 and BT2109 are separated by 23 nucleotides
BT2107: BT2107 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,645,309 to 2,648,461
BT2107
BT2108: BT2108 - hypothetical protein (NCBI ptt file), at 2,648,568 to 2,650,397
BT2108
BT2109: BT2109 - hypothetical protein (NCBI ptt file), at 2,650,421 to 2,651,620
BT2109
Position (kb)
2648
2649
2650
2651 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2647.612 kb on + strand, within BT2107 at 2647.647 kb on + strand, within BT2107 at 2647.654 kb on - strand, within BT2107 at 2647.696 kb on - strand, within BT2107 at 2647.696 kb on - strand, within BT2107 at 2647.712 kb on + strand, within BT2107 at 2647.727 kb on + strand, within BT2107 at 2647.737 kb on + strand, within BT2107 at 2647.896 kb on - strand, within BT2107 at 2647.898 kb on - strand, within BT2107 at 2647.975 kb on - strand, within BT2107 at 2648.000 kb on + strand, within BT2107 at 2648.119 kb on + strand, within BT2107 at 2648.120 kb on - strand, within BT2107 at 2648.132 kb on + strand, within BT2107 at 2648.145 kb on - strand, within BT2107 at 2648.167 kb on + strand at 2648.168 kb on - strand at 2648.227 kb on + strand at 2648.253 kb on - strand at 2648.263 kb on - strand at 2648.284 kb on + strand at 2648.285 kb on - strand at 2648.285 kb on - strand at 2648.313 kb on + strand at 2648.314 kb on - strand at 2648.388 kb on + strand at 2648.389 kb on - strand at 2648.389 kb on - strand at 2648.452 kb on - strand at 2648.493 kb on - strand at 2648.605 kb on + strand at 2648.686 kb on - strand at 2648.712 kb on - strand at 2648.768 kb on - strand, within BT2108 at 2648.807 kb on - strand, within BT2108 at 2648.886 kb on - strand, within BT2108 at 2648.919 kb on + strand, within BT2108 at 2648.925 kb on - strand, within BT2108 at 2648.995 kb on + strand, within BT2108 at 2648.995 kb on + strand, within BT2108 at 2648.996 kb on - strand, within BT2108 at 2648.999 kb on + strand, within BT2108 at 2649.042 kb on + strand, within BT2108 at 2649.203 kb on + strand, within BT2108 at 2649.203 kb on + strand, within BT2108 at 2649.210 kb on + strand, within BT2108 at 2649.239 kb on - strand, within BT2108 at 2649.261 kb on - strand, within BT2108 at 2649.358 kb on + strand, within BT2108 at 2649.382 kb on + strand, within BT2108 at 2649.382 kb on + strand, within BT2108 at 2649.400 kb on + strand, within BT2108 at 2649.440 kb on - strand, within BT2108 at 2649.448 kb on + strand, within BT2108 at 2649.448 kb on + strand, within BT2108 at 2649.448 kb on + strand, within BT2108 at 2649.481 kb on + strand, within BT2108 at 2649.496 kb on + strand, within BT2108 at 2649.504 kb on + strand, within BT2108 at 2649.504 kb on + strand, within BT2108 at 2649.531 kb on - strand, within BT2108 at 2649.634 kb on - strand, within BT2108 at 2649.635 kb on + strand, within BT2108 at 2649.665 kb on + strand, within BT2108 at 2649.674 kb on + strand, within BT2108 at 2649.675 kb on - strand, within BT2108 at 2649.732 kb on + strand, within BT2108 at 2649.733 kb on - strand, within BT2108 at 2649.799 kb on - strand, within BT2108 at 2649.847 kb on + strand, within BT2108 at 2649.847 kb on + strand, within BT2108 at 2649.848 kb on - strand, within BT2108 at 2649.848 kb on - strand, within BT2108 at 2649.848 kb on - strand, within BT2108 at 2649.864 kb on + strand, within BT2108 at 2649.964 kb on - strand, within BT2108 at 2649.971 kb on - strand, within BT2108 at 2650.018 kb on + strand, within BT2108 at 2650.019 kb on - strand, within BT2108 at 2650.019 kb on - strand, within BT2108 at 2650.021 kb on - strand, within BT2108 at 2650.023 kb on + strand, within BT2108 at 2650.023 kb on + strand, within BT2108 at 2650.024 kb on - strand, within BT2108 at 2650.024 kb on - strand, within BT2108 at 2650.026 kb on + strand, within BT2108 at 2650.030 kb on + strand, within BT2108 at 2650.223 kb on + strand at 2650.224 kb on - strand at 2650.224 kb on - strand at 2650.285 kb on - strand at 2650.292 kb on - strand at 2650.292 kb on - strand at 2650.334 kb on - strand at 2650.344 kb on - strand at 2650.396 kb on - strand at 2650.408 kb on - strand at 2650.447 kb on - strand at 2650.449 kb on + strand at 2650.450 kb on - strand at 2650.488 kb on - strand at 2650.488 kb on - strand at 2650.543 kb on + strand, within BT2109 at 2650.543 kb on + strand, within BT2109 at 2650.544 kb on - strand, within BT2109 at 2650.579 kb on + strand, within BT2109 at 2650.613 kb on + strand, within BT2109 at 2650.624 kb on + strand, within BT2109 at 2650.633 kb on + strand, within BT2109 at 2650.637 kb on - strand, within BT2109 at 2650.637 kb on - strand, within BT2109 at 2650.660 kb on - strand, within BT2109 at 2650.825 kb on - strand, within BT2109 at 2650.944 kb on + strand, within BT2109 at 2650.944 kb on + strand, within BT2109 at 2650.945 kb on - strand, within BT2109 at 2650.981 kb on - strand, within BT2109 at 2650.996 kb on + strand, within BT2109 at 2650.997 kb on - strand, within BT2109 at 2651.029 kb on + strand, within BT2109 at 2651.057 kb on + strand, within BT2109 at 2651.058 kb on - strand, within BT2109 at 2651.088 kb on + strand, within BT2109 at 2651.157 kb on - strand, within BT2109 at 2651.185 kb on - strand, within BT2109 at 2651.206 kb on + strand, within BT2109 at 2651.243 kb on - strand, within BT2109 at 2651.311 kb on - strand, within BT2109 at 2651.311 kb on - strand, within BT2109
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,647,612 + BT2107 0.73 -1.1 2,647,647 + BT2107 0.74 -2.6 2,647,654 - BT2107 0.74 -2.3 2,647,696 - BT2107 0.76 -1.6 2,647,696 - BT2107 0.76 -2.4 2,647,712 + BT2107 0.76 -0.1 2,647,727 + BT2107 0.77 -1.1 2,647,737 + BT2107 0.77 -0.5 2,647,896 - BT2107 0.82 -2.8 2,647,898 - BT2107 0.82 -1.6 2,647,975 - BT2107 0.85 -1.7 2,648,000 + BT2107 0.85 -0.1 2,648,119 + BT2107 0.89 -1.6 2,648,120 - BT2107 0.89 -0.1 2,648,132 + BT2107 0.90 -1.6 2,648,145 - BT2107 0.90 -0.3 2,648,167 + -3.2 2,648,168 - -1.4 2,648,227 + -0.5 2,648,253 - -1.6 2,648,263 - -0.1 2,648,284 + -2.6 2,648,285 - -0.1 2,648,285 - +0.8 2,648,313 + -0.6 2,648,314 - -1.1 2,648,388 + -3.4 2,648,389 - -2.1 2,648,389 - -0.1 2,648,452 - -0.1 2,648,493 - -0.6 2,648,605 + -2.2 2,648,686 - -1.1 2,648,712 - -1.7 2,648,768 - BT2108 0.11 -2.1 2,648,807 - BT2108 0.13 -1.1 2,648,886 - BT2108 0.17 -1.4 2,648,919 + BT2108 0.19 -3.4 2,648,925 - BT2108 0.20 +0.9 2,648,995 + BT2108 0.23 -1.1 2,648,995 + BT2108 0.23 -1.6 2,648,996 - BT2108 0.23 -3.8 2,648,999 + BT2108 0.24 -2.4 2,649,042 + BT2108 0.26 -2.9 2,649,203 + BT2108 0.35 -0.6 2,649,203 + BT2108 0.35 -2.6 2,649,210 + BT2108 0.35 -1.3 2,649,239 - BT2108 0.37 -2.1 2,649,261 - BT2108 0.38 -1.1 2,649,358 + BT2108 0.43 -0.8 2,649,382 + BT2108 0.44 -2.8 2,649,382 + BT2108 0.44 -0.1 2,649,400 + BT2108 0.45 -2.1 2,649,440 - BT2108 0.48 -2.6 2,649,448 + BT2108 0.48 +1.4 2,649,448 + BT2108 0.48 -3.2 2,649,448 + BT2108 0.48 -2.1 2,649,481 + BT2108 0.50 +0.9 2,649,496 + BT2108 0.51 -1.3 2,649,504 + BT2108 0.51 -1.1 2,649,504 + BT2108 0.51 -0.8 2,649,531 - BT2108 0.53 -1.1 2,649,634 - BT2108 0.58 -0.1 2,649,635 + BT2108 0.58 -0.1 2,649,665 + BT2108 0.60 -1.1 2,649,674 + BT2108 0.60 -3.1 2,649,675 - BT2108 0.60 -2.4 2,649,732 + BT2108 0.64 -4.8 2,649,733 - BT2108 0.64 -2.6 2,649,799 - BT2108 0.67 -2.3 2,649,847 + BT2108 0.70 -2.6 2,649,847 + BT2108 0.70 +1.3 2,649,848 - BT2108 0.70 -0.1 2,649,848 - BT2108 0.70 -3.9 2,649,848 - BT2108 0.70 -1.6 2,649,864 + BT2108 0.71 -3.8 2,649,964 - BT2108 0.76 -1.9 2,649,971 - BT2108 0.77 -0.8 2,650,018 + BT2108 0.79 -2.1 2,650,019 - BT2108 0.79 -0.1 2,650,019 - BT2108 0.79 -2.1 2,650,021 - BT2108 0.79 -2.4 2,650,023 + BT2108 0.80 -2.9 2,650,023 + BT2108 0.80 +1.3 2,650,024 - BT2108 0.80 +0.3 2,650,024 - BT2108 0.80 -4.5 2,650,026 + BT2108 0.80 -1.6 2,650,030 + BT2108 0.80 -0.1 2,650,223 + -2.9 2,650,224 - -0.1 2,650,224 - +1.5 2,650,285 - -3.4 2,650,292 - -2.4 2,650,292 - -3.0 2,650,334 - +1.0 2,650,344 - -1.0 2,650,396 - -1.4 2,650,408 - -3.0 2,650,447 - -1.0 2,650,449 + -1.5 2,650,450 - +3.4 2,650,488 - -1.0 2,650,488 - -1.3 2,650,543 + BT2109 0.10 -2.0 2,650,543 + BT2109 0.10 -0.0 2,650,544 - BT2109 0.10 -1.8 2,650,579 + BT2109 0.13 -2.9 2,650,613 + BT2109 0.16 -0.6 2,650,624 + BT2109 0.17 -1.6 2,650,633 + BT2109 0.18 -1.0 2,650,637 - BT2109 0.18 +1.0 2,650,637 - BT2109 0.18 +1.5 2,650,660 - BT2109 0.20 -1.6 2,650,825 - BT2109 0.34 -0.0 2,650,944 + BT2109 0.44 -2.9 2,650,944 + BT2109 0.44 -0.0 2,650,945 - BT2109 0.44 +1.0 2,650,981 - BT2109 0.47 -2.0 2,650,996 + BT2109 0.48 -1.0 2,650,997 - BT2109 0.48 -1.6 2,651,029 + BT2109 0.51 -1.0 2,651,057 + BT2109 0.53 -2.4 2,651,058 - BT2109 0.53 -0.0 2,651,088 + BT2109 0.56 -2.4 2,651,157 - BT2109 0.61 +3.0 2,651,185 - BT2109 0.64 -2.5 2,651,206 + BT2109 0.65 -1.6 2,651,243 - BT2109 0.69 -2.6 2,651,311 - BT2109 0.74 -1.6 2,651,311 - BT2109 0.74 -3.5
Or see this region's nucleotide sequence