Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1677 and BT1678 overlap by 20 nucleotides BT1678 and BT1679 are separated by 7 nucleotides BT1679 and BT1680 are separated by 2 nucleotides BT1680 and BT1681 are separated by 398 nucleotides
BT1677: BT1677 - putative hydrolase (NCBI ptt file), at 2,070,593 to 2,071,114
BT1677
BT1678: BT1678 - conserved hypothetical protein (NCBI ptt file), at 2,071,095 to 2,071,889
BT1678
BT1679: BT1679 - hypothetical protein (NCBI ptt file), at 2,071,897 to 2,072,232
BT1679
BT1680: BT1680 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 2,072,235 to 2,072,771
BT1680
BT1681: BT1681 - putative carbonic anhydrase (NCBI ptt file), at 2,073,170 to 2,073,712
BT1681
Position (kb)
2071
2072
2073 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2072.120 kb on - strand, within BT1679 at 2072.143 kb on - strand, within BT1679 at 2072.200 kb on - strand at 2072.212 kb on - strand at 2072.470 kb on - strand, within BT1680 at 2072.487 kb on - strand, within BT1680 at 2072.640 kb on - strand, within BT1680 at 2072.646 kb on - strand, within BT1680 at 2072.646 kb on - strand, within BT1680 at 2072.707 kb on - strand, within BT1680 at 2072.796 kb on - strand at 2072.796 kb on - strand at 2072.796 kb on - strand at 2072.800 kb on - strand at 2072.826 kb on - strand at 2072.844 kb on - strand at 2072.844 kb on - strand at 2072.861 kb on + strand at 2072.870 kb on + strand at 2072.964 kb on + strand at 2072.992 kb on + strand at 2072.993 kb on - strand at 2073.077 kb on - strand at 2073.078 kb on - strand at 2073.137 kb on - strand at 2073.150 kb on + strand at 2073.173 kb on + strand at 2073.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,072,120 - BT1679 0.66 -3.3 2,072,143 - BT1679 0.73 -2.8 2,072,200 - -0.0 2,072,212 - -3.5 2,072,470 - BT1680 0.44 -0.4 2,072,487 - BT1680 0.47 -2.3 2,072,640 - BT1680 0.75 -1.6 2,072,646 - BT1680 0.77 -0.0 2,072,646 - BT1680 0.77 +3.5 2,072,707 - BT1680 0.88 -2.0 2,072,796 - -1.6 2,072,796 - -2.3 2,072,796 - -3.2 2,072,800 - -2.2 2,072,826 - -2.0 2,072,844 - -2.3 2,072,844 - -2.6 2,072,861 + -2.0 2,072,870 + -1.7 2,072,964 + -1.7 2,072,992 + +1.0 2,072,993 - -1.0 2,073,077 - -0.0 2,073,078 - -1.0 2,073,137 - -3.0 2,073,150 + -3.8 2,073,173 + -1.0 2,073,206 + -1.0
Or see this region's nucleotide sequence