Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1141

Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1139 and BT1140 are separated by 150 nucleotidesBT1140 and BT1141 are separated by 309 nucleotidesBT1141 and BT1142 are separated by 9 nucleotidesBT1142 and BT1143 are separated by 214 nucleotides BT1139: BT1139 - conserved hypothetical protein (NCBI ptt file), at 1,423,594 to 1,424,652 BT1139 BT1140: BT1140 - hypothetical protein (NCBI ptt file), at 1,424,803 to 1,425,201 BT1140 BT1141: BT1141 - cation efflux family protein (NCBI ptt file), at 1,425,511 to 1,426,434 BT1141 BT1142: BT1142 - hypothetical protein (NCBI ptt file), at 1,426,444 to 1,426,929 BT1142 BT1143: BT1143 - conserved hypothetical protein (NCBI ptt file), at 1,427,144 to 1,427,344 BT1143 Position (kb) 1425 1426 1427Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1424.527 kb on - strand, within BT1139at 1424.558 kb on - strandat 1424.657 kb on - strandat 1424.911 kb on + strand, within BT1140at 1424.996 kb on + strand, within BT1140at 1424.997 kb on - strand, within BT1140at 1425.049 kb on - strand, within BT1140at 1425.060 kb on + strand, within BT1140at 1425.129 kb on + strand, within BT1140at 1425.129 kb on + strand, within BT1140at 1425.133 kb on + strand, within BT1140at 1425.153 kb on + strand, within BT1140at 1425.222 kb on + strandat 1425.244 kb on + strandat 1425.300 kb on + strandat 1425.303 kb on + strandat 1425.362 kb on + strandat 1425.415 kb on + strandat 1425.448 kb on - strandat 1425.478 kb on - strandat 1425.478 kb on - strandat 1425.479 kb on + strandat 1425.480 kb on - strandat 1425.482 kb on - strandat 1425.534 kb on - strandat 1425.562 kb on - strandat 1425.600 kb on - strandat 1425.725 kb on + strand, within BT1141at 1425.780 kb on - strand, within BT1141at 1425.813 kb on + strand, within BT1141at 1425.823 kb on - strand, within BT1141at 1425.868 kb on + strand, within BT1141at 1425.869 kb on - strand, within BT1141at 1425.911 kb on + strand, within BT1141at 1425.912 kb on - strand, within BT1141at 1425.912 kb on - strand, within BT1141at 1425.942 kb on + strand, within BT1141at 1425.943 kb on - strand, within BT1141at 1425.943 kb on - strand, within BT1141at 1425.987 kb on + strandat 1425.988 kb on - strand, within BT1141at 1426.047 kb on - strand, within BT1141at 1426.067 kb on + strand, within BT1141at 1426.084 kb on - strand, within BT1141at 1426.164 kb on + strand, within BT1141at 1426.341 kb on - strand, within BT1141at 1426.351 kb on + strandat 1426.357 kb on - strandat 1426.362 kb on - strandat 1426.364 kb on + strandat 1426.457 kb on - strandat 1426.457 kb on - strandat 1426.458 kb on + strandat 1426.683 kb on + strand, within BT1142at 1426.684 kb on - strand, within BT1142at 1426.686 kb on - strand, within BT1142at 1426.747 kb on - strand, within BT1142at 1426.762 kb on - strand, within BT1142at 1426.825 kb on + strand, within BT1142at 1426.860 kb on + strand, within BT1142at 1426.860 kb on + strand, within BT1142at 1426.910 kb on - strandat 1426.929 kb on + strandat 1426.990 kb on + strandat 1427.070 kb on + strandat 1427.121 kb on - strandat 1427.121 kb on - strandat 1427.157 kb on - strandat 1427.176 kb on + strand, within BT1143at 1427.246 kb on - strand, within BT1143at 1427.249 kb on - strand, within BT1143at 1427.323 kb on + strand, within BT1143at 1427.367 kb on - strandat 1427.426 kb on + strandat 1427.431 kb on + strandat 1427.432 kb on - strandat 1427.432 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
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1,424,527 - BT1139 0.88 -1.5
1,424,558 - -1.5
1,424,657 - -1.0
1,424,911 + BT1140 0.27 +1.8
1,424,996 + BT1140 0.48 -2.3
1,424,997 - BT1140 0.49 -1.5
1,425,049 - BT1140 0.62 +0.0
1,425,060 + BT1140 0.64 +0.8
1,425,129 + BT1140 0.82 -1.3
1,425,129 + BT1140 0.82 -1.5
1,425,133 + BT1140 0.83 -1.5
1,425,153 + BT1140 0.88 -1.0
1,425,222 + -1.0
1,425,244 + -1.0
1,425,300 + +0.0
1,425,303 + +0.0
1,425,362 + +0.6
1,425,415 + -1.3
1,425,448 - -2.0
1,425,478 - +2.4
1,425,478 - -0.8
1,425,479 + +0.0
1,425,480 - -1.0
1,425,482 - -1.0
1,425,534 - -1.0
1,425,562 - -1.0
1,425,600 - -2.0
1,425,725 + BT1141 0.23 -3.5
1,425,780 - BT1141 0.29 -2.3
1,425,813 + BT1141 0.33 -1.0
1,425,823 - BT1141 0.34 -2.3
1,425,868 + BT1141 0.39 -2.5
1,425,869 - BT1141 0.39 -2.9
1,425,911 + BT1141 0.43 +0.0
1,425,912 - BT1141 0.43 -1.0
1,425,912 - BT1141 0.43 +0.0
1,425,942 + BT1141 0.47 -2.0
1,425,943 - BT1141 0.47 -1.5
1,425,943 - BT1141 0.47 -2.2
1,425,987 + +0.0
1,425,988 - BT1141 0.52 -3.5
1,426,047 - BT1141 0.58 -1.5
1,426,067 + BT1141 0.60 +0.0
1,426,084 - BT1141 0.62 -2.0
1,426,164 + BT1141 0.71 +0.0
1,426,341 - BT1141 0.90 -1.0
1,426,351 + +2.0
1,426,357 - +2.3
1,426,362 - -1.6
1,426,364 + -1.6
1,426,457 - -2.0
1,426,457 - -2.6
1,426,458 + -2.6
1,426,683 + BT1142 0.49 +1.6
1,426,684 - BT1142 0.49 -1.0
1,426,686 - BT1142 0.50 -2.8
1,426,747 - BT1142 0.62 -1.0
1,426,762 - BT1142 0.65 -1.0
1,426,825 + BT1142 0.78 -2.9
1,426,860 + BT1142 0.86 +0.6
1,426,860 + BT1142 0.86 -2.3
1,426,910 - +0.0
1,426,929 + -0.8
1,426,990 + -1.6
1,427,070 + -1.6
1,427,121 - -1.0
1,427,121 - -2.0
1,427,157 - +0.6
1,427,176 + BT1143 0.16 -2.8
1,427,246 - BT1143 0.51 +0.0
1,427,249 - BT1143 0.52 -1.6
1,427,323 + BT1143 0.89 +1.0
1,427,367 - +1.6
1,427,426 + -1.0
1,427,431 + -3.3
1,427,432 - -2.0
1,427,432 - -1.0

Or see this region's nucleotide sequence