Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0048 and BT0049 are separated by 8 nucleotides BT0049 and BT0050 are separated by 11 nucleotides BT0050 and BT0051 overlap by 1 nucleotides
BT0048: BT0048 - hypothetical protein (NCBI ptt file), at 44,162 to 44,638
BT0048
BT0049: BT0049 - putative glycosyltransferase (NCBI ptt file), at 44,647 to 45,582
BT0049
BT0050: BT0050 - putative glycosyltransferase (NCBI ptt file), at 45,594 to 46,565
BT0050
BT0051: BT0051 - putative glycosyltransferase (NCBI ptt file), at 46,565 to 47,545
BT0051
Position (kb) 45
46
47 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 44.600 kb on - strand at 44.782 kb on + strand, within BT0049 at 44.784 kb on + strand, within BT0049 at 44.893 kb on + strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 44.978 kb on - strand, within BT0049 at 45.042 kb on - strand, within BT0049 at 45.089 kb on - strand, within BT0049 at 45.119 kb on + strand, within BT0049 at 45.155 kb on + strand, within BT0049 at 45.201 kb on - strand, within BT0049 at 45.259 kb on - strand, within BT0049 at 45.270 kb on - strand, within BT0049 at 45.301 kb on + strand, within BT0049 at 45.337 kb on - strand, within BT0049 at 45.370 kb on - strand, within BT0049 at 45.423 kb on + strand, within BT0049 at 45.423 kb on + strand, within BT0049 at 45.550 kb on + strand at 45.567 kb on - strand at 45.630 kb on + strand at 45.661 kb on + strand at 45.703 kb on + strand, within BT0050 at 45.731 kb on + strand, within BT0050 at 45.742 kb on + strand, within BT0050 at 45.743 kb on - strand, within BT0050 at 45.943 kb on - strand, within BT0050 at 45.981 kb on + strand, within BT0050 at 46.014 kb on - strand, within BT0050 at 46.162 kb on - strand, within BT0050 at 46.213 kb on - strand, within BT0050 at 46.218 kb on - strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.364 kb on + strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.365 kb on - strand, within BT0050 at 46.366 kb on + strand, within BT0050 at 46.367 kb on - strand, within BT0050 at 46.382 kb on + strand, within BT0050 at 46.459 kb on - strand, within BT0050 at 46.484 kb on + strand at 46.558 kb on - strand at 46.560 kb on + strand at 46.593 kb on - strand at 46.593 kb on - strand at 46.593 kb on - strand at 46.600 kb on - strand at 46.647 kb on - strand at 46.662 kb on - strand at 46.662 kb on - strand at 46.672 kb on - strand, within BT0051 at 46.698 kb on + strand, within BT0051 at 46.755 kb on + strand, within BT0051 at 46.826 kb on + strand, within BT0051 at 46.916 kb on - strand, within BT0051 at 47.011 kb on - strand, within BT0051 at 47.042 kb on + strand, within BT0051 at 47.043 kb on - strand, within BT0051 at 47.064 kb on - strand, within BT0051 at 47.068 kb on - strand, within BT0051 at 47.103 kb on + strand, within BT0051 at 47.122 kb on - strand, within BT0051 at 47.416 kb on + strand, within BT0051 at 47.512 kb on - strand at 47.559 kb on + strand at 47.565 kb on - strand at 47.565 kb on - strand at 47.565 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 44,600 - -1.5 44,782 + BT0049 0.14 -1.0 44,784 + BT0049 0.15 +0.0 44,893 + BT0049 0.26 +1.0 44,978 - BT0049 0.35 -1.2 44,978 - BT0049 0.35 -1.0 44,978 - BT0049 0.35 +0.1 45,042 - BT0049 0.42 -1.5 45,089 - BT0049 0.47 -1.5 45,119 + BT0049 0.50 +0.6 45,155 + BT0049 0.54 -0.5 45,201 - BT0049 0.59 -1.0 45,259 - BT0049 0.65 +0.5 45,270 - BT0049 0.67 -2.8 45,301 + BT0049 0.70 +0.0 45,337 - BT0049 0.74 -1.5 45,370 - BT0049 0.77 +0.0 45,423 + BT0049 0.83 -2.0 45,423 + BT0049 0.83 +1.6 45,550 + -2.5 45,567 - -0.5 45,630 + -1.5 45,661 + -3.0 45,703 + BT0050 0.11 -2.3 45,731 + BT0050 0.14 -2.4 45,742 + BT0050 0.15 +2.0 45,743 - BT0050 0.15 -2.3 45,943 - BT0050 0.36 -2.0 45,981 + BT0050 0.40 -2.8 46,014 - BT0050 0.43 -3.5 46,162 - BT0050 0.58 -2.0 46,213 - BT0050 0.64 -1.0 46,218 - BT0050 0.64 -3.0 46,364 + BT0050 0.79 +0.0 46,364 + BT0050 0.79 -2.3 46,365 - BT0050 0.79 -2.2 46,365 - BT0050 0.79 +0.6 46,366 + BT0050 0.79 -1.3 46,367 - BT0050 0.80 -1.9 46,382 + BT0050 0.81 -3.1 46,459 - BT0050 0.89 -3.8 46,484 + -4.5 46,558 - -2.0 46,560 + +0.0 46,593 - -0.7 46,593 - -1.2 46,593 - -1.0 46,600 - +0.0 46,647 - +0.0 46,662 - -1.8 46,662 - -1.3 46,672 - BT0051 0.11 -2.0 46,698 + BT0051 0.14 -1.8 46,755 + BT0051 0.19 -2.3 46,826 + BT0051 0.27 -2.0 46,916 - BT0051 0.36 -1.0 47,011 - BT0051 0.45 -1.5 47,042 + BT0051 0.49 +0.0 47,043 - BT0051 0.49 +1.6 47,064 - BT0051 0.51 -2.5 47,068 - BT0051 0.51 -3.7 47,103 + BT0051 0.55 +1.6 47,122 - BT0051 0.57 -1.0 47,416 + BT0051 0.87 +0.0 47,512 - -0.9 47,559 + +0.4 47,565 - +0.6 47,565 - -3.0 47,565 - -1.0
Or see this region's nucleotide sequence