Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4724 and BT4725 are separated by 12 nucleotides BT4725 and BT4726 are separated by 21 nucleotides
BT4724: BT4724 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 6,198,720 to 6,202,139
BT4724
BT4725: BT4725 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 6,202,152 to 6,203,495
BT4725
BT4726: BT4726 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 6,203,517 to 6,206,048
BT4726
Position (kb)
6202
6203
6204 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6201.159 kb on - strand, within BT4724 at 6201.167 kb on - strand, within BT4724 at 6201.195 kb on - strand, within BT4724 at 6201.399 kb on - strand, within BT4724 at 6201.399 kb on - strand, within BT4724 at 6201.496 kb on - strand, within BT4724 at 6201.759 kb on - strand, within BT4724 at 6201.856 kb on - strand at 6201.922 kb on + strand at 6201.922 kb on + strand at 6202.113 kb on - strand at 6202.167 kb on - strand at 6202.176 kb on + strand at 6202.177 kb on - strand at 6202.177 kb on - strand at 6202.184 kb on - strand at 6202.184 kb on - strand at 6202.202 kb on - strand at 6202.255 kb on + strand at 6202.436 kb on - strand, within BT4725 at 6202.468 kb on + strand, within BT4725 at 6202.575 kb on + strand, within BT4725 at 6202.575 kb on + strand, within BT4725 at 6202.580 kb on + strand, within BT4725 at 6202.615 kb on + strand, within BT4725 at 6202.616 kb on - strand, within BT4725 at 6202.625 kb on + strand, within BT4725 at 6202.630 kb on - strand, within BT4725 at 6202.706 kb on - strand, within BT4725 at 6202.748 kb on - strand, within BT4725 at 6202.748 kb on - strand, within BT4725 at 6202.830 kb on - strand, within BT4725 at 6202.954 kb on - strand, within BT4725 at 6202.965 kb on - strand, within BT4725 at 6203.012 kb on + strand, within BT4725 at 6203.013 kb on - strand, within BT4725 at 6203.022 kb on + strand, within BT4725 at 6203.052 kb on - strand, within BT4725 at 6203.157 kb on - strand, within BT4725 at 6203.157 kb on - strand, within BT4725 at 6203.159 kb on - strand, within BT4725 at 6203.425 kb on - strand at 6203.635 kb on - strand at 6203.635 kb on - strand at 6203.635 kb on - strand at 6203.691 kb on + strand at 6203.754 kb on + strand at 6203.755 kb on - strand at 6203.772 kb on + strand, within BT4726 at 6203.912 kb on - strand, within BT4726 at 6203.912 kb on - strand, within BT4726 at 6203.952 kb on + strand, within BT4726 at 6203.952 kb on + strand, within BT4726 at 6203.953 kb on - strand, within BT4726 at 6203.953 kb on - strand, within BT4726 at 6203.973 kb on + strand, within BT4726 at 6204.068 kb on + strand, within BT4726 at 6204.069 kb on - strand, within BT4726 at 6204.069 kb on - strand, within BT4726 at 6204.072 kb on - strand, within BT4726 at 6204.072 kb on - strand, within BT4726 at 6204.089 kb on - strand, within BT4726 at 6204.103 kb on - strand, within BT4726 at 6204.103 kb on - strand, within BT4726 at 6204.237 kb on - strand, within BT4726 at 6204.274 kb on + strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.275 kb on - strand, within BT4726 at 6204.276 kb on + strand, within BT4726 at 6204.276 kb on + strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.277 kb on - strand, within BT4726 at 6204.278 kb on + strand, within BT4726 at 6204.278 kb on + strand, within BT4726 at 6204.297 kb on - strand, within BT4726 at 6204.320 kb on + strand, within BT4726 at 6204.321 kb on - strand, within BT4726 at 6204.363 kb on - strand, within BT4726 at 6204.401 kb on + strand, within BT4726 at 6204.401 kb on - strand, within BT4726 at 6204.491 kb on - strand, within BT4726 at 6204.491 kb on - strand, within BT4726
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 6,201,159 - BT4724 0.71 +1.0 6,201,167 - BT4724 0.72 -1.0 6,201,195 - BT4724 0.72 -2.0 6,201,399 - BT4724 0.78 +0.0 6,201,399 - BT4724 0.78 -1.0 6,201,496 - BT4724 0.81 -0.4 6,201,759 - BT4724 0.89 +0.0 6,201,856 - -2.3 6,201,922 + -2.6 6,201,922 + -2.3 6,202,113 - -1.0 6,202,167 - +0.0 6,202,176 + -1.6 6,202,177 - -2.2 6,202,177 - -1.0 6,202,184 - -2.7 6,202,184 - +0.0 6,202,202 - +1.1 6,202,255 + -0.2 6,202,436 - BT4725 0.21 -1.0 6,202,468 + BT4725 0.24 -1.0 6,202,575 + BT4725 0.31 +1.6 6,202,575 + BT4725 0.31 +2.6 6,202,580 + BT4725 0.32 +0.6 6,202,615 + BT4725 0.34 -0.2 6,202,616 - BT4725 0.35 +0.0 6,202,625 + BT4725 0.35 +0.0 6,202,630 - BT4725 0.36 -1.0 6,202,706 - BT4725 0.41 -2.3 6,202,748 - BT4725 0.44 +0.0 6,202,748 - BT4725 0.44 -1.3 6,202,830 - BT4725 0.50 -1.0 6,202,954 - BT4725 0.60 +0.0 6,202,965 - BT4725 0.60 +0.0 6,203,012 + BT4725 0.64 +0.0 6,203,013 - BT4725 0.64 -1.6 6,203,022 + BT4725 0.65 +1.2 6,203,052 - BT4725 0.67 -1.0 6,203,157 - BT4725 0.75 +0.0 6,203,157 - BT4725 0.75 -3.3 6,203,159 - BT4725 0.75 -1.0 6,203,425 - -3.0 6,203,635 - -1.9 6,203,635 - +2.4 6,203,635 - -1.6 6,203,691 + +1.3 6,203,754 + +1.0 6,203,755 - -1.0 6,203,772 + BT4726 0.10 -2.6 6,203,912 - BT4726 0.16 -2.0 6,203,912 - BT4726 0.16 -1.4 6,203,952 + BT4726 0.17 -1.0 6,203,952 + BT4726 0.17 -1.0 6,203,953 - BT4726 0.17 -1.1 6,203,953 - BT4726 0.17 -1.0 6,203,973 + BT4726 0.18 -2.3 6,204,068 + BT4726 0.22 -1.3 6,204,069 - BT4726 0.22 -1.5 6,204,069 - BT4726 0.22 -1.0 6,204,072 - BT4726 0.22 -0.8 6,204,072 - BT4726 0.22 -1.8 6,204,089 - BT4726 0.23 -1.0 6,204,103 - BT4726 0.23 -1.2 6,204,103 - BT4726 0.23 +0.0 6,204,237 - BT4726 0.28 -4.3 6,204,274 + BT4726 0.30 -2.6 6,204,275 - BT4726 0.30 -1.2 6,204,275 - BT4726 0.30 -2.7 6,204,275 - BT4726 0.30 +0.0 6,204,276 + BT4726 0.30 -1.6 6,204,276 + BT4726 0.30 +0.0 6,204,277 - BT4726 0.30 +0.0 6,204,277 - BT4726 0.30 -2.0 6,204,277 - BT4726 0.30 +0.0 6,204,277 - BT4726 0.30 -0.8 6,204,278 + BT4726 0.30 -1.6 6,204,278 + BT4726 0.30 -1.0 6,204,297 - BT4726 0.31 -0.8 6,204,320 + BT4726 0.32 -1.6 6,204,321 - BT4726 0.32 -1.3 6,204,363 - BT4726 0.33 -1.0 6,204,401 + BT4726 0.35 -1.6 6,204,401 - BT4726 0.35 -2.0 6,204,491 - BT4726 0.38 -2.0 6,204,491 - BT4726 0.38 -1.7
Or see this region's nucleotide sequence