Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4519 and BT4520 are separated by 45 nucleotides BT4520 and BT4521 are separated by 29 nucleotides BT4521 and BT4522 are separated by 6 nucleotides
BT4519: BT4519 - conserved hypothetical protein (NCBI ptt file), at 5,938,387 to 5,939,517
BT4519
BT4520: BT4520 - Type I restriction enzyme EcoAI specificity protein (NCBI ptt file), at 5,939,563 to 5,940,987
BT4520
BT4521: BT4521 - integrase (NCBI ptt file), at 5,941,017 to 5,941,820
BT4521
BT4522: BT4522 - Type I restriction-modification enzyme (NCBI ptt file), at 5,941,827 to 5,942,354
BT4522
Position (kb)
5939
5940
5941 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5938.615 kb on - strand, within BT4519 at 5938.653 kb on + strand, within BT4519 at 5938.654 kb on - strand, within BT4519 at 5938.762 kb on - strand, within BT4519 at 5938.798 kb on + strand, within BT4519 at 5938.844 kb on - strand, within BT4519 at 5938.850 kb on + strand, within BT4519 at 5938.876 kb on - strand, within BT4519 at 5938.887 kb on + strand, within BT4519 at 5938.978 kb on - strand, within BT4519 at 5939.018 kb on + strand, within BT4519 at 5939.127 kb on - strand, within BT4519 at 5939.205 kb on - strand, within BT4519 at 5939.212 kb on + strand, within BT4519 at 5939.212 kb on + strand, within BT4519 at 5939.220 kb on + strand, within BT4519 at 5939.275 kb on + strand, within BT4519 at 5939.275 kb on + strand, within BT4519 at 5939.297 kb on - strand, within BT4519 at 5939.332 kb on - strand, within BT4519 at 5939.336 kb on + strand, within BT4519 at 5939.399 kb on - strand, within BT4519 at 5939.399 kb on - strand, within BT4519 at 5939.399 kb on - strand, within BT4519 at 5939.515 kb on + strand at 5939.519 kb on - strand at 5939.569 kb on - strand at 5939.607 kb on - strand at 5939.607 kb on - strand at 5939.621 kb on + strand at 5939.622 kb on - strand at 5939.743 kb on - strand, within BT4520 at 5939.789 kb on + strand, within BT4520 at 5940.010 kb on + strand, within BT4520 at 5940.010 kb on + strand, within BT4520 at 5940.074 kb on + strand, within BT4520 at 5940.104 kb on + strand, within BT4520 at 5940.121 kb on - strand, within BT4520 at 5940.160 kb on - strand, within BT4520 at 5940.191 kb on + strand, within BT4520 at 5940.238 kb on - strand, within BT4520 at 5940.252 kb on - strand, within BT4520 at 5940.351 kb on - strand, within BT4520 at 5940.358 kb on - strand, within BT4520 at 5940.362 kb on + strand, within BT4520 at 5940.362 kb on + strand, within BT4520 at 5940.363 kb on - strand, within BT4520 at 5940.365 kb on + strand, within BT4520 at 5940.365 kb on + strand, within BT4520 at 5940.448 kb on - strand, within BT4520 at 5940.470 kb on + strand, within BT4520 at 5940.475 kb on - strand, within BT4520 at 5940.492 kb on + strand, within BT4520 at 5940.509 kb on + strand, within BT4520 at 5940.509 kb on + strand, within BT4520 at 5940.515 kb on + strand, within BT4520 at 5940.555 kb on + strand, within BT4520 at 5940.555 kb on + strand, within BT4520 at 5940.555 kb on + strand, within BT4520 at 5940.557 kb on + strand, within BT4520 at 5940.557 kb on + strand, within BT4520 at 5940.557 kb on + strand, within BT4520 at 5940.633 kb on + strand, within BT4520 at 5940.669 kb on - strand, within BT4520 at 5940.669 kb on - strand, within BT4520 at 5940.669 kb on - strand, within BT4520 at 5940.669 kb on - strand, within BT4520 at 5940.671 kb on + strand, within BT4520 at 5940.674 kb on + strand, within BT4520 at 5940.727 kb on + strand, within BT4520 at 5940.776 kb on - strand, within BT4520 at 5940.784 kb on + strand, within BT4520 at 5940.785 kb on - strand, within BT4520 at 5940.790 kb on + strand, within BT4520 at 5940.793 kb on + strand, within BT4520 at 5940.793 kb on + strand, within BT4520 at 5940.794 kb on - strand, within BT4520 at 5940.794 kb on - strand, within BT4520 at 5940.794 kb on - strand, within BT4520 at 5940.814 kb on + strand, within BT4520 at 5940.815 kb on - strand, within BT4520 at 5940.883 kb on - strand at 5940.994 kb on - strand at 5941.030 kb on - strand at 5941.109 kb on + strand, within BT4521 at 5941.123 kb on - strand, within BT4521 at 5941.123 kb on - strand, within BT4521 at 5941.256 kb on + strand at 5941.257 kb on - strand, within BT4521 at 5941.286 kb on + strand, within BT4521 at 5941.287 kb on - strand, within BT4521 at 5941.357 kb on - strand, within BT4521 at 5941.365 kb on + strand, within BT4521 at 5941.471 kb on - strand, within BT4521 at 5941.481 kb on - strand, within BT4521 at 5941.491 kb on - strand, within BT4521 at 5941.507 kb on - strand, within BT4521 at 5941.573 kb on - strand, within BT4521 at 5941.632 kb on - strand, within BT4521 at 5941.688 kb on + strand, within BT4521 at 5941.689 kb on - strand, within BT4521 at 5941.691 kb on - strand, within BT4521 at 5941.764 kb on - strand at 5941.833 kb on + strand at 5941.858 kb on + strand at 5941.858 kb on + strand at 5941.858 kb on + strand at 5941.863 kb on + strand at 5941.866 kb on - strand at 5941.878 kb on - strand at 5941.878 kb on - strand at 5941.919 kb on - strand, within BT4522 at 5941.921 kb on + strand, within BT4522
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 5,938,615 - BT4519 0.20 -1.0 5,938,653 + BT4519 0.24 +1.1 5,938,654 - BT4519 0.24 +1.0 5,938,762 - BT4519 0.33 -1.7 5,938,798 + BT4519 0.36 -1.3 5,938,844 - BT4519 0.40 -3.0 5,938,850 + BT4519 0.41 +2.0 5,938,876 - BT4519 0.43 +2.2 5,938,887 + BT4519 0.44 -2.8 5,938,978 - BT4519 0.52 +0.0 5,939,018 + BT4519 0.56 +0.0 5,939,127 - BT4519 0.65 +1.0 5,939,205 - BT4519 0.72 +0.0 5,939,212 + BT4519 0.73 +0.6 5,939,212 + BT4519 0.73 -1.6 5,939,220 + BT4519 0.74 -2.3 5,939,275 + BT4519 0.79 -3.2 5,939,275 + BT4519 0.79 -1.6 5,939,297 - BT4519 0.80 -2.4 5,939,332 - BT4519 0.84 -2.0 5,939,336 + BT4519 0.84 +1.0 5,939,399 - BT4519 0.89 +0.0 5,939,399 - BT4519 0.89 +0.6 5,939,399 - BT4519 0.89 -2.6 5,939,515 + +0.0 5,939,519 - -2.7 5,939,569 - -1.6 5,939,607 - -0.6 5,939,607 - -0.6 5,939,621 + +0.0 5,939,622 - +1.0 5,939,743 - BT4520 0.13 -0.6 5,939,789 + BT4520 0.16 -0.4 5,940,010 + BT4520 0.31 -0.5 5,940,010 + BT4520 0.31 -2.5 5,940,074 + BT4520 0.36 +0.0 5,940,104 + BT4520 0.38 +0.3 5,940,121 - BT4520 0.39 -1.0 5,940,160 - BT4520 0.42 -2.0 5,940,191 + BT4520 0.44 -1.0 5,940,238 - BT4520 0.47 +1.6 5,940,252 - BT4520 0.48 +2.1 5,940,351 - BT4520 0.55 -0.4 5,940,358 - BT4520 0.56 +3.0 5,940,362 + BT4520 0.56 -2.6 5,940,362 + BT4520 0.56 -1.7 5,940,363 - BT4520 0.56 -0.7 5,940,365 + BT4520 0.56 -2.7 5,940,365 + BT4520 0.56 -1.0 5,940,448 - BT4520 0.62 -1.0 5,940,470 + BT4520 0.64 -2.6 5,940,475 - BT4520 0.64 -1.9 5,940,492 + BT4520 0.65 -2.8 5,940,509 + BT4520 0.66 -1.0 5,940,509 + BT4520 0.66 +0.0 5,940,515 + BT4520 0.67 -0.7 5,940,555 + BT4520 0.70 -2.2 5,940,555 + BT4520 0.70 -1.9 5,940,555 + BT4520 0.70 -2.2 5,940,557 + BT4520 0.70 -1.0 5,940,557 + BT4520 0.70 +0.0 5,940,557 + BT4520 0.70 -2.2 5,940,633 + BT4520 0.75 +1.1 5,940,669 - BT4520 0.78 -1.3 5,940,669 - BT4520 0.78 -0.3 5,940,669 - BT4520 0.78 -2.3 5,940,669 - BT4520 0.78 -1.4 5,940,671 + BT4520 0.78 -1.8 5,940,674 + BT4520 0.78 -1.8 5,940,727 + BT4520 0.82 -3.3 5,940,776 - BT4520 0.85 -1.6 5,940,784 + BT4520 0.86 +0.0 5,940,785 - BT4520 0.86 -1.1 5,940,790 + BT4520 0.86 +0.0 5,940,793 + BT4520 0.86 -1.4 5,940,793 + BT4520 0.86 +0.0 5,940,794 - BT4520 0.86 +0.6 5,940,794 - BT4520 0.86 -0.7 5,940,794 - BT4520 0.86 +1.6 5,940,814 + BT4520 0.88 -1.8 5,940,815 - BT4520 0.88 +0.0 5,940,883 - -1.6 5,940,994 - -1.2 5,941,030 - +1.2 5,941,109 + BT4521 0.11 +0.0 5,941,123 - BT4521 0.13 -3.8 5,941,123 - BT4521 0.13 +0.4 5,941,256 + -1.0 5,941,257 - BT4521 0.30 -1.0 5,941,286 + BT4521 0.33 +0.6 5,941,287 - BT4521 0.34 -1.8 5,941,357 - BT4521 0.42 +0.0 5,941,365 + BT4521 0.43 -0.7 5,941,471 - BT4521 0.56 -0.2 5,941,481 - BT4521 0.58 -4.8 5,941,491 - BT4521 0.59 -2.2 5,941,507 - BT4521 0.61 -1.0 5,941,573 - BT4521 0.69 +0.6 5,941,632 - BT4521 0.76 -1.6 5,941,688 + BT4521 0.83 -1.2 5,941,689 - BT4521 0.84 -1.0 5,941,691 - BT4521 0.84 -0.8 5,941,764 - -1.7 5,941,833 + -0.7 5,941,858 + +3.0 5,941,858 + +1.6 5,941,858 + +0.0 5,941,863 + -2.3 5,941,866 - +0.1 5,941,878 - -1.0 5,941,878 - -2.9 5,941,919 - BT4522 0.17 +0.3 5,941,921 + BT4522 0.18 -2.6
Or see this region's nucleotide sequence