Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4004 and BT4005 are separated by 91 nucleotides BT4005 and BT4006 are separated by 112 nucleotides BT4006 and BT4007 are separated by 16 nucleotides
BT4004: BT4004 - lipid-A-disaccharide synthase (NCBI ptt file), at 5,216,850 to 5,217,986
BT4004
BT4005: BT4005 - hypothetical protein (NCBI ptt file), at 5,218,078 to 5,218,854
BT4005
BT4006: BT4006 - phosphatidate cytidylyltransferase (NCBI ptt file), at 5,218,967 to 5,219,809
BT4006
BT4007: BT4007 - AAA-metalloprotease FtsH, with ATPase domain (NCBI ptt file), at 5,219,826 to 5,221,916
BT4007
Position (kb) 5218
5219 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5218.002 kb on + strand at 5218.003 kb on - strand at 5218.044 kb on - strand at 5218.045 kb on + strand at 5218.045 kb on + strand at 5218.047 kb on + strand at 5218.047 kb on + strand at 5218.101 kb on - strand at 5218.157 kb on - strand, within BT4005 at 5218.172 kb on + strand, within BT4005 at 5218.220 kb on - strand, within BT4005 at 5218.277 kb on - strand, within BT4005 at 5218.302 kb on - strand, within BT4005 at 5218.396 kb on - strand, within BT4005 at 5218.434 kb on + strand, within BT4005 at 5218.498 kb on + strand, within BT4005 at 5218.535 kb on + strand, within BT4005 at 5218.536 kb on - strand, within BT4005 at 5218.547 kb on + strand, within BT4005 at 5218.600 kb on + strand, within BT4005 at 5218.600 kb on + strand, within BT4005 at 5218.602 kb on + strand, within BT4005 at 5218.602 kb on + strand, within BT4005 at 5218.604 kb on + strand, within BT4005 at 5218.604 kb on + strand, within BT4005 at 5218.605 kb on - strand, within BT4005 at 5218.626 kb on + strand, within BT4005 at 5218.626 kb on + strand, within BT4005 at 5218.627 kb on - strand, within BT4005 at 5218.630 kb on + strand, within BT4005 at 5218.631 kb on - strand, within BT4005 at 5218.690 kb on + strand, within BT4005 at 5218.690 kb on + strand, within BT4005 at 5218.746 kb on + strand, within BT4005 at 5218.746 kb on + strand, within BT4005 at 5218.849 kb on - strand at 5218.870 kb on + strand at 5218.949 kb on - strand at 5219.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 5,218,002 + -0.5 5,218,003 - +1.1 5,218,044 - -0.4 5,218,045 + -1.3 5,218,045 + -4.2 5,218,047 + -3.0 5,218,047 + -1.6 5,218,101 - -0.7 5,218,157 - BT4005 0.10 -1.8 5,218,172 + BT4005 0.12 -2.3 5,218,220 - BT4005 0.18 -3.2 5,218,277 - BT4005 0.26 -2.8 5,218,302 - BT4005 0.29 -2.8 5,218,396 - BT4005 0.41 -3.5 5,218,434 + BT4005 0.46 -0.0 5,218,498 + BT4005 0.54 -3.3 5,218,535 + BT4005 0.59 -3.5 5,218,536 - BT4005 0.59 -2.5 5,218,547 + BT4005 0.60 -4.8 5,218,600 + BT4005 0.67 -1.0 5,218,600 + BT4005 0.67 -2.5 5,218,602 + BT4005 0.67 -2.3 5,218,602 + BT4005 0.67 -1.0 5,218,604 + BT4005 0.68 -1.6 5,218,604 + BT4005 0.68 -1.2 5,218,605 - BT4005 0.68 +1.0 5,218,626 + BT4005 0.71 -2.0 5,218,626 + BT4005 0.71 -1.6 5,218,627 - BT4005 0.71 -3.5 5,218,630 + BT4005 0.71 -1.6 5,218,631 - BT4005 0.71 -1.9 5,218,690 + BT4005 0.79 -0.0 5,218,690 + BT4005 0.79 -3.8 5,218,746 + BT4005 0.86 -2.8 5,218,746 + BT4005 0.86 -1.0 5,218,849 - -1.4 5,218,870 + -1.9 5,218,949 - -1.8 5,219,832 - -0.0
Or see this region's nucleotide sequence