Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3079 and BT3080 are separated by 92 nucleotides BT3080 and BT3081 are separated by 127 nucleotides BT3081 and BT3082 are separated by 52 nucleotides
BT3079: BT3079 - conserved hypothetical protein (NCBI ptt file), at 3,903,322 to 3,905,082
BT3079
BT3080: BT3080 - cysteine synthase A (NCBI ptt file), at 3,905,175 to 3,906,122
BT3080
BT3081: BT3081 - putative pectin degradation protein (NCBI ptt file), at 3,906,250 to 3,906,591
BT3081
BT3082: BT3082 - 2,6-beta-D-fructofuranosidase precursor (NCBI ptt file), at 3,906,644 to 3,908,290
BT3082
Position (kb)
3905
3906
3907 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 3904.187 kb on - strand, within BT3079 at 3904.187 kb on - strand, within BT3079 at 3904.189 kb on + strand, within BT3079 at 3904.313 kb on + strand, within BT3079 at 3904.313 kb on + strand, within BT3079 at 3904.313 kb on + strand, within BT3079 at 3904.313 kb on + strand, within BT3079 at 3904.314 kb on - strand, within BT3079 at 3904.314 kb on - strand, within BT3079 at 3904.314 kb on - strand, within BT3079 at 3904.314 kb on - strand, within BT3079 at 3904.362 kb on - strand, within BT3079 at 3904.371 kb on + strand, within BT3079 at 3904.378 kb on + strand, within BT3079 at 3904.389 kb on + strand, within BT3079 at 3904.432 kb on - strand, within BT3079 at 3904.537 kb on + strand, within BT3079 at 3904.546 kb on + strand, within BT3079 at 3904.546 kb on + strand, within BT3079 at 3904.546 kb on + strand, within BT3079 at 3904.547 kb on - strand, within BT3079 at 3904.608 kb on - strand, within BT3079 at 3904.615 kb on + strand, within BT3079 at 3904.615 kb on + strand, within BT3079 at 3904.620 kb on + strand, within BT3079 at 3904.620 kb on + strand, within BT3079 at 3904.621 kb on - strand, within BT3079 at 3904.621 kb on - strand, within BT3079 at 3904.668 kb on + strand, within BT3079 at 3904.733 kb on + strand, within BT3079 at 3904.733 kb on + strand, within BT3079 at 3904.734 kb on - strand, within BT3079 at 3904.834 kb on + strand, within BT3079 at 3904.835 kb on - strand, within BT3079 at 3904.851 kb on - strand, within BT3079 at 3904.860 kb on + strand, within BT3079 at 3904.886 kb on + strand, within BT3079 at 3904.886 kb on + strand, within BT3079 at 3904.886 kb on + strand, within BT3079 at 3904.886 kb on + strand, within BT3079 at 3904.887 kb on - strand, within BT3079 at 3904.921 kb on - strand at 3904.979 kb on + strand at 3904.988 kb on - strand at 3904.988 kb on - strand at 3905.049 kb on + strand at 3905.049 kb on + strand at 3905.082 kb on + strand at 3905.082 kb on + strand at 3905.085 kb on + strand at 3905.088 kb on + strand at 3905.089 kb on - strand at 3905.131 kb on - strand at 3905.142 kb on + strand at 3905.142 kb on + strand at 3905.149 kb on + strand at 3905.151 kb on + strand at 3905.176 kb on + strand at 3905.176 kb on + strand at 3905.185 kb on + strand at 3905.186 kb on - strand at 3905.200 kb on + strand at 3905.208 kb on - strand at 3905.210 kb on + strand at 3905.211 kb on - strand at 3905.211 kb on - strand at 3905.218 kb on + strand at 3905.219 kb on - strand at 3905.248 kb on - strand at 3905.260 kb on - strand at 3905.300 kb on - strand, within BT3080 at 3905.300 kb on - strand, within BT3080 at 3905.319 kb on + strand, within BT3080 at 3905.320 kb on - strand, within BT3080 at 3905.418 kb on - strand, within BT3080 at 3905.508 kb on + strand, within BT3080 at 3905.565 kb on - strand, within BT3080 at 3905.584 kb on - strand, within BT3080 at 3905.628 kb on + strand, within BT3080 at 3905.649 kb on + strand, within BT3080 at 3905.689 kb on - strand, within BT3080 at 3905.693 kb on + strand, within BT3080 at 3905.852 kb on - strand, within BT3080 at 3905.891 kb on + strand, within BT3080 at 3905.985 kb on + strand, within BT3080 at 3906.050 kb on + strand at 3906.050 kb on + strand at 3906.056 kb on - strand at 3906.059 kb on + strand at 3906.060 kb on - strand at 3906.060 kb on - strand at 3906.060 kb on - strand at 3906.083 kb on + strand at 3906.084 kb on - strand at 3906.123 kb on - strand at 3906.124 kb on + strand at 3906.124 kb on + strand at 3906.125 kb on - strand at 3906.197 kb on - strand at 3906.198 kb on + strand at 3906.199 kb on - strand at 3906.199 kb on - strand at 3906.235 kb on - strand at 3906.235 kb on - strand at 3906.251 kb on + strand at 3906.252 kb on - strand at 3906.290 kb on - strand, within BT3081 at 3906.295 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.340 kb on + strand, within BT3081 at 3906.341 kb on - strand, within BT3081 at 3906.341 kb on - strand, within BT3081 at 3906.341 kb on - strand, within BT3081 at 3906.344 kb on + strand, within BT3081 at 3906.348 kb on - strand at 3906.368 kb on - strand, within BT3081 at 3906.368 kb on - strand, within BT3081 at 3906.368 kb on - strand, within BT3081 at 3906.416 kb on + strand, within BT3081 at 3906.416 kb on + strand, within BT3081 at 3906.416 kb on + strand, within BT3081 at 3906.434 kb on + strand, within BT3081 at 3906.451 kb on - strand, within BT3081 at 3906.451 kb on - strand, within BT3081 at 3906.592 kb on - strand at 3906.608 kb on - strand at 3906.759 kb on + strand at 3906.759 kb on + strand at 3906.759 kb on + strand at 3906.760 kb on - strand at 3906.776 kb on - strand at 3906.779 kb on + strand at 3906.779 kb on + strand at 3906.779 kb on + strand at 3906.788 kb on - strand at 3906.804 kb on + strand at 3906.805 kb on - strand at 3906.808 kb on + strand at 3906.978 kb on + strand, within BT3082 at 3906.978 kb on + strand, within BT3082 at 3906.978 kb on + strand, within BT3082 at 3906.979 kb on - strand, within BT3082 at 3907.042 kb on + strand, within BT3082 at 3907.043 kb on - strand, within BT3082 at 3907.043 kb on - strand, within BT3082 at 3907.043 kb on - strand, within BT3082 at 3907.102 kb on + strand, within BT3082
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 3,904,187 - BT3079 0.49 -1.6 3,904,187 - BT3079 0.49 -2.8 3,904,189 + BT3079 0.49 -1.8 3,904,313 + BT3079 0.56 -1.6 3,904,313 + BT3079 0.56 -3.0 3,904,313 + BT3079 0.56 -3.6 3,904,313 + BT3079 0.56 -2.3 3,904,314 - BT3079 0.56 -1.0 3,904,314 - BT3079 0.56 -0.8 3,904,314 - BT3079 0.56 -0.4 3,904,314 - BT3079 0.56 -2.8 3,904,362 - BT3079 0.59 -2.0 3,904,371 + BT3079 0.60 -1.3 3,904,378 + BT3079 0.60 -0.4 3,904,389 + BT3079 0.61 -1.8 3,904,432 - BT3079 0.63 -2.7 3,904,537 + BT3079 0.69 -3.1 3,904,546 + BT3079 0.70 -1.0 3,904,546 + BT3079 0.70 +1.0 3,904,546 + BT3079 0.70 -0.0 3,904,547 - BT3079 0.70 +1.1 3,904,608 - BT3079 0.73 -3.4 3,904,615 + BT3079 0.73 +2.3 3,904,615 + BT3079 0.73 -0.5 3,904,620 + BT3079 0.74 -3.2 3,904,620 + BT3079 0.74 -2.4 3,904,621 - BT3079 0.74 -0.0 3,904,621 - BT3079 0.74 -1.5 3,904,668 + BT3079 0.76 +3.4 3,904,733 + BT3079 0.80 -2.3 3,904,733 + BT3079 0.80 -1.0 3,904,734 - BT3079 0.80 -1.7 3,904,834 + BT3079 0.86 -0.8 3,904,835 - BT3079 0.86 +1.3 3,904,851 - BT3079 0.87 -0.0 3,904,860 + BT3079 0.87 -2.0 3,904,886 + BT3079 0.89 -2.8 3,904,886 + BT3079 0.89 -1.7 3,904,886 + BT3079 0.89 -1.0 3,904,886 + BT3079 0.89 -1.2 3,904,887 - BT3079 0.89 -1.6 3,904,921 - -3.8 3,904,979 + -0.0 3,904,988 - -1.0 3,904,988 - -1.6 3,905,049 + -2.6 3,905,049 + -2.4 3,905,082 + -2.6 3,905,082 + -0.7 3,905,085 + -2.0 3,905,088 + -0.0 3,905,089 - -0.0 3,905,131 - -2.0 3,905,142 + -1.4 3,905,142 + +1.2 3,905,149 + +1.0 3,905,151 + -2.6 3,905,176 + -1.2 3,905,176 + -1.0 3,905,185 + -1.0 3,905,186 - -2.6 3,905,200 + -1.0 3,905,208 - -0.0 3,905,210 + -0.8 3,905,211 - -1.7 3,905,211 - -1.0 3,905,218 + -4.0 3,905,219 - -2.0 3,905,248 - -4.6 3,905,260 - -3.6 3,905,300 - BT3080 0.13 -0.0 3,905,300 - BT3080 0.13 -0.0 3,905,319 + BT3080 0.15 -3.5 3,905,320 - BT3080 0.15 -4.9 3,905,418 - BT3080 0.26 -1.0 3,905,508 + BT3080 0.35 -4.6 3,905,565 - BT3080 0.41 -2.4 3,905,584 - BT3080 0.43 -1.6 3,905,628 + BT3080 0.48 -2.6 3,905,649 + BT3080 0.50 -5.0 3,905,689 - BT3080 0.54 -6.0 3,905,693 + BT3080 0.55 -6.4 3,905,852 - BT3080 0.71 -4.4 3,905,891 + BT3080 0.76 -5.5 3,905,985 + BT3080 0.85 -4.6 3,906,050 + -5.1 3,906,050 + -3.8 3,906,056 - -1.0 3,906,059 + -3.2 3,906,060 - -5.5 3,906,060 - -5.1 3,906,060 - -0.0 3,906,083 + -4.0 3,906,084 - -3.9 3,906,123 - +1.6 3,906,124 + -4.0 3,906,124 + -2.4 3,906,125 - -2.1 3,906,197 - -0.6 3,906,198 + -3.2 3,906,199 - -2.4 3,906,199 - -1.0 3,906,235 - -1.7 3,906,235 - -1.4 3,906,251 + +2.0 3,906,252 - -0.4 3,906,290 - BT3081 0.12 -1.4 3,906,295 + BT3081 0.13 -0.7 3,906,340 + BT3081 0.26 -0.0 3,906,340 + BT3081 0.26 -0.0 3,906,340 + BT3081 0.26 -1.0 3,906,340 + BT3081 0.26 -2.8 3,906,340 + BT3081 0.26 -1.6 3,906,340 + BT3081 0.26 -1.0 3,906,340 + BT3081 0.26 -1.3 3,906,341 - BT3081 0.27 -1.4 3,906,341 - BT3081 0.27 -0.0 3,906,341 - BT3081 0.27 -1.0 3,906,344 + BT3081 0.27 -2.3 3,906,348 - -1.6 3,906,368 - BT3081 0.35 -2.6 3,906,368 - BT3081 0.35 -1.3 3,906,368 - BT3081 0.35 +0.3 3,906,416 + BT3081 0.49 +2.6 3,906,416 + BT3081 0.49 +0.1 3,906,416 + BT3081 0.49 +0.4 3,906,434 + BT3081 0.54 +0.2 3,906,451 - BT3081 0.59 -1.0 3,906,451 - BT3081 0.59 -0.0 3,906,592 - -0.0 3,906,608 - -1.0 3,906,759 + +0.6 3,906,759 + -1.9 3,906,759 + -2.3 3,906,760 - -1.6 3,906,776 - -3.4 3,906,779 + -1.0 3,906,779 + -1.6 3,906,779 + -1.6 3,906,788 - +0.7 3,906,804 + +3.0 3,906,805 - +0.8 3,906,808 + -0.8 3,906,978 + BT3082 0.20 -2.0 3,906,978 + BT3082 0.20 -2.4 3,906,978 + BT3082 0.20 -1.1 3,906,979 - BT3082 0.20 -0.0 3,907,042 + BT3082 0.24 -1.0 3,907,043 - BT3082 0.24 -1.9 3,907,043 - BT3082 0.24 -0.7 3,907,043 - BT3082 0.24 -4.5 3,907,102 + BT3082 0.28 -1.6
Or see this region's nucleotide sequence