Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2955 and BT2956 overlap by 4 nucleotides BT2956 and BT2957 are separated by 197 nucleotides
BT2955: BT2955 - formate acetyltransferase 2 (NCBI ptt file), at 3,721,561 to 3,723,705
BT2955
BT2956: BT2956 - putative pyruvate formate-lyase 3 activating enzyme (NCBI ptt file), at 3,723,702 to 3,724,601
BT2956
BT2957: BT2957 - transcriptional regulator (NCBI ptt file), at 3,724,799 to 3,725,635
BT2957
Position (kb)
3723
3724
3725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3722.714 kb on + strand, within BT2955 at 3722.784 kb on + strand, within BT2955 at 3722.971 kb on + strand, within BT2955 at 3723.008 kb on + strand, within BT2955 at 3723.029 kb on + strand, within BT2955 at 3723.034 kb on + strand, within BT2955 at 3723.034 kb on + strand, within BT2955 at 3723.058 kb on + strand, within BT2955 at 3723.124 kb on - strand, within BT2955 at 3723.156 kb on + strand, within BT2955 at 3723.156 kb on + strand, within BT2955 at 3723.156 kb on + strand, within BT2955 at 3723.157 kb on - strand, within BT2955 at 3723.158 kb on + strand, within BT2955 at 3723.181 kb on + strand, within BT2955 at 3723.198 kb on + strand, within BT2955 at 3723.202 kb on + strand, within BT2955 at 3723.202 kb on + strand, within BT2955 at 3723.202 kb on + strand, within BT2955 at 3723.202 kb on + strand, within BT2955 at 3723.202 kb on + strand, within BT2955 at 3723.203 kb on - strand, within BT2955 at 3723.203 kb on - strand, within BT2955 at 3723.203 kb on - strand, within BT2955 at 3723.203 kb on - strand, within BT2955 at 3723.272 kb on - strand, within BT2955 at 3723.299 kb on - strand, within BT2955 at 3723.318 kb on - strand, within BT2955 at 3723.395 kb on + strand, within BT2955 at 3723.395 kb on + strand, within BT2955 at 3723.443 kb on + strand, within BT2955 at 3723.444 kb on - strand, within BT2955 at 3723.481 kb on - strand, within BT2955 at 3723.485 kb on - strand, within BT2955 at 3723.565 kb on + strand at 3723.566 kb on - strand at 3723.566 kb on - strand at 3723.606 kb on - strand at 3723.695 kb on + strand at 3723.696 kb on - strand at 3723.727 kb on + strand at 3723.738 kb on + strand at 3723.873 kb on + strand, within BT2956 at 3723.894 kb on + strand, within BT2956 at 3723.945 kb on - strand, within BT2956 at 3723.998 kb on + strand, within BT2956 at 3724.032 kb on + strand, within BT2956 at 3724.033 kb on - strand, within BT2956 at 3724.068 kb on + strand, within BT2956 at 3724.128 kb on + strand, within BT2956 at 3724.128 kb on + strand, within BT2956 at 3724.129 kb on - strand, within BT2956 at 3724.129 kb on - strand, within BT2956 at 3724.140 kb on + strand, within BT2956 at 3724.140 kb on + strand, within BT2956 at 3724.140 kb on + strand, within BT2956 at 3724.141 kb on - strand, within BT2956 at 3724.145 kb on + strand, within BT2956 at 3724.146 kb on - strand, within BT2956 at 3724.169 kb on + strand, within BT2956 at 3724.218 kb on - strand, within BT2956 at 3724.243 kb on + strand, within BT2956 at 3724.247 kb on + strand, within BT2956 at 3724.269 kb on + strand, within BT2956 at 3724.270 kb on - strand, within BT2956 at 3724.270 kb on - strand, within BT2956 at 3724.270 kb on - strand, within BT2956 at 3724.406 kb on - strand, within BT2956 at 3724.406 kb on - strand, within BT2956 at 3724.414 kb on - strand, within BT2956 at 3724.420 kb on - strand, within BT2956 at 3724.421 kb on + strand, within BT2956 at 3724.421 kb on + strand, within BT2956 at 3724.538 kb on + strand at 3724.589 kb on + strand at 3724.604 kb on + strand at 3724.640 kb on + strand at 3724.771 kb on + strand at 3724.821 kb on + strand at 3724.870 kb on + strand at 3724.870 kb on + strand at 3724.872 kb on + strand at 3724.872 kb on + strand at 3724.873 kb on - strand at 3724.914 kb on + strand, within BT2957 at 3724.993 kb on - strand, within BT2957 at 3725.002 kb on - strand, within BT2957 at 3725.002 kb on - strand, within BT2957 at 3725.002 kb on - strand, within BT2957 at 3725.020 kb on + strand, within BT2957 at 3725.103 kb on - strand, within BT2957 at 3725.116 kb on + strand, within BT2957 at 3725.116 kb on + strand, within BT2957 at 3725.140 kb on + strand, within BT2957 at 3725.174 kb on - strand, within BT2957 at 3725.233 kb on - strand, within BT2957 at 3725.272 kb on + strand, within BT2957 at 3725.272 kb on + strand, within BT2957 at 3725.273 kb on - strand, within BT2957 at 3725.319 kb on + strand, within BT2957 at 3725.329 kb on + strand, within BT2957 at 3725.389 kb on - strand, within BT2957 at 3725.398 kb on + strand, within BT2957 at 3725.399 kb on - strand, within BT2957 at 3725.402 kb on - strand, within BT2957 at 3725.416 kb on + strand, within BT2957 at 3725.417 kb on - strand, within BT2957 at 3725.461 kb on - strand, within BT2957 at 3725.481 kb on - strand, within BT2957 at 3725.489 kb on - strand, within BT2957 at 3725.500 kb on + strand, within BT2957 at 3725.510 kb on - strand, within BT2957 at 3725.526 kb on + strand, within BT2957 at 3725.527 kb on - strand, within BT2957 at 3725.531 kb on - strand, within BT2957 at 3725.567 kb on - strand at 3725.571 kb on + strand at 3725.585 kb on + strand at 3725.587 kb on + strand at 3725.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 3,722,714 + BT2955 0.54 -3.3 3,722,784 + BT2955 0.57 -2.6 3,722,971 + BT2955 0.66 -0.1 3,723,008 + BT2955 0.67 -1.6 3,723,029 + BT2955 0.68 -1.6 3,723,034 + BT2955 0.69 -2.4 3,723,034 + BT2955 0.69 -1.6 3,723,058 + BT2955 0.70 +0.4 3,723,124 - BT2955 0.73 -1.9 3,723,156 + BT2955 0.74 -2.8 3,723,156 + BT2955 0.74 -0.5 3,723,156 + BT2955 0.74 +0.9 3,723,157 - BT2955 0.74 -0.1 3,723,158 + BT2955 0.74 -0.1 3,723,181 + BT2955 0.76 -3.2 3,723,198 + BT2955 0.76 -1.6 3,723,202 + BT2955 0.77 -2.1 3,723,202 + BT2955 0.77 -0.3 3,723,202 + BT2955 0.77 -3.4 3,723,202 + BT2955 0.77 +0.7 3,723,202 + BT2955 0.77 -1.1 3,723,203 - BT2955 0.77 -1.4 3,723,203 - BT2955 0.77 -2.5 3,723,203 - BT2955 0.77 -2.2 3,723,203 - BT2955 0.77 -1.9 3,723,272 - BT2955 0.80 -0.1 3,723,299 - BT2955 0.81 -2.7 3,723,318 - BT2955 0.82 -0.1 3,723,395 + BT2955 0.86 -1.9 3,723,395 + BT2955 0.86 -2.6 3,723,443 + BT2955 0.88 -1.1 3,723,444 - BT2955 0.88 -3.1 3,723,481 - BT2955 0.90 -0.1 3,723,485 - BT2955 0.90 -1.4 3,723,565 + -2.3 3,723,566 - -0.1 3,723,566 - -0.1 3,723,606 - -2.6 3,723,695 + -1.8 3,723,696 - -0.8 3,723,727 + -1.4 3,723,738 + -0.6 3,723,873 + BT2956 0.19 -0.1 3,723,894 + BT2956 0.21 -1.6 3,723,945 - BT2956 0.27 -1.6 3,723,998 + BT2956 0.33 -1.1 3,724,032 + BT2956 0.37 -2.4 3,724,033 - BT2956 0.37 -2.4 3,724,068 + BT2956 0.41 -0.5 3,724,128 + BT2956 0.47 -1.4 3,724,128 + BT2956 0.47 -0.8 3,724,129 - BT2956 0.47 -1.6 3,724,129 - BT2956 0.47 +2.3 3,724,140 + BT2956 0.49 -1.6 3,724,140 + BT2956 0.49 -0.1 3,724,140 + BT2956 0.49 -0.1 3,724,141 - BT2956 0.49 -0.1 3,724,145 + BT2956 0.49 +0.7 3,724,146 - BT2956 0.49 -1.0 3,724,169 + BT2956 0.52 -0.1 3,724,218 - BT2956 0.57 -2.0 3,724,243 + BT2956 0.60 -1.6 3,724,247 + BT2956 0.61 -2.5 3,724,269 + BT2956 0.63 +0.7 3,724,270 - BT2956 0.63 -1.1 3,724,270 - BT2956 0.63 -1.6 3,724,270 - BT2956 0.63 -0.1 3,724,406 - BT2956 0.78 +0.6 3,724,406 - BT2956 0.78 -0.1 3,724,414 - BT2956 0.79 -0.2 3,724,420 - BT2956 0.80 +2.7 3,724,421 + BT2956 0.80 +0.5 3,724,421 + BT2956 0.80 -0.1 3,724,538 + -3.2 3,724,589 + -3.3 3,724,604 + -3.0 3,724,640 + +0.2 3,724,771 + -0.3 3,724,821 + -2.3 3,724,870 + -1.6 3,724,870 + -1.1 3,724,872 + -0.6 3,724,872 + -1.1 3,724,873 - -0.5 3,724,914 + BT2957 0.14 +1.3 3,724,993 - BT2957 0.23 -2.1 3,725,002 - BT2957 0.24 -1.1 3,725,002 - BT2957 0.24 -0.6 3,725,002 - BT2957 0.24 -2.4 3,725,020 + BT2957 0.26 -2.4 3,725,103 - BT2957 0.36 +0.9 3,725,116 + BT2957 0.38 -2.6 3,725,116 + BT2957 0.38 -2.3 3,725,140 + BT2957 0.41 -1.6 3,725,174 - BT2957 0.45 -0.1 3,725,233 - BT2957 0.52 +0.6 3,725,272 + BT2957 0.57 -1.5 3,725,272 + BT2957 0.57 -1.9 3,725,273 - BT2957 0.57 -1.1 3,725,319 + BT2957 0.62 -1.0 3,725,329 + BT2957 0.63 -2.6 3,725,389 - BT2957 0.70 -2.5 3,725,398 + BT2957 0.72 +0.1 3,725,399 - BT2957 0.72 +0.5 3,725,402 - BT2957 0.72 +1.9 3,725,416 + BT2957 0.74 -1.6 3,725,417 - BT2957 0.74 -0.6 3,725,461 - BT2957 0.79 -0.6 3,725,481 - BT2957 0.81 +0.8 3,725,489 - BT2957 0.82 -0.1 3,725,500 + BT2957 0.84 -0.1 3,725,510 - BT2957 0.85 -0.1 3,725,526 + BT2957 0.87 -2.9 3,725,527 - BT2957 0.87 -0.1 3,725,531 - BT2957 0.87 -2.4 3,725,567 - -0.3 3,725,571 + -2.1 3,725,585 + -0.6 3,725,587 + -2.2 3,725,588 - -1.1
Or see this region's nucleotide sequence