Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2740 and tRNA-Thr are separated by 50 nucleotides tRNA-Thr and tRNA-Gly are separated by 12 nucleotides tRNA-Gly and tRNA-Tyr are separated by 25 nucleotides tRNA-Tyr and tRNA-Thr are separated by 155 nucleotides tRNA-Thr and BT2741 are separated by 97 nucleotides BT2741 and BT2742 are separated by 16 nucleotides
BT2740: BT2740 - elongation factor Tu (NCBI ptt file), at 3,393,922 to 3,395,106
BT2740
BT_t45: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,157 to 3,395,228
tRNA-Thr
BT_t46: tRNA-Gly - tRNA-Gly (RefSeq), at 3,395,241 to 3,395,313
tRNA-Gly
BT_t47: tRNA-Tyr - tRNA-Tyr (RefSeq), at 3,395,339 to 3,395,424
tRNA-Tyr
BT_t48: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,580 to 3,395,656
tRNA-Thr
BT2741: BT2741 - sigma-54 modulation protein (NCBI ptt file), at 3,395,754 to 3,396,053
BT2741
BT2742: BT2742 - integrase, site-specific recombinase (NCBI ptt file), at 3,396,070 to 3,396,951
BT2742
Position (kb)
3395
3396
3397 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3395.763 kb on - strand at 3395.763 kb on - strand at 3395.838 kb on - strand, within BT2741 at 3395.849 kb on + strand, within BT2741 at 3395.850 kb on - strand, within BT2741 at 3395.921 kb on + strand, within BT2741 at 3395.921 kb on + strand, within BT2741 at 3395.944 kb on - strand, within BT2741 at 3395.944 kb on - strand at 3396.045 kb on - strand at 3396.054 kb on - strand at 3396.054 kb on - strand at 3396.058 kb on - strand at 3396.058 kb on - strand at 3396.223 kb on - strand, within BT2742
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 3,395,763 - -0.5 3,395,763 - +1.8 3,395,838 - BT2741 0.28 +0.6 3,395,849 + BT2741 0.32 -2.0 3,395,850 - BT2741 0.32 +2.4 3,395,921 + BT2741 0.56 +2.6 3,395,921 + BT2741 0.56 -0.6 3,395,944 - BT2741 0.63 +4.4 3,395,944 - +1.0 3,396,045 - +0.7 3,396,054 - +2.4 3,396,054 - +2.8 3,396,058 - +2.9 3,396,058 - -2.0 3,396,223 - BT2742 0.17 +1.2
Or see this region's nucleotide sequence