Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2741

Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2740 and tRNA-Thr are separated by 50 nucleotidestRNA-Thr and tRNA-Gly are separated by 12 nucleotidestRNA-Gly and tRNA-Tyr are separated by 25 nucleotidestRNA-Tyr and tRNA-Thr are separated by 155 nucleotidestRNA-Thr and BT2741 are separated by 97 nucleotidesBT2741 and BT2742 are separated by 16 nucleotides BT2740: BT2740 - elongation factor Tu (NCBI ptt file), at 3,393,922 to 3,395,106 BT2740 BT_t45: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,157 to 3,395,228 tRNA-Thr BT_t46: tRNA-Gly - tRNA-Gly (RefSeq), at 3,395,241 to 3,395,313 tRNA-Gly BT_t47: tRNA-Tyr - tRNA-Tyr (RefSeq), at 3,395,339 to 3,395,424 tRNA-Tyr BT_t48: tRNA-Thr - tRNA-Thr (RefSeq), at 3,395,580 to 3,395,656 tRNA-Thr BT2741: BT2741 - sigma-54 modulation protein (NCBI ptt file), at 3,395,754 to 3,396,053 BT2741 BT2742: BT2742 - integrase, site-specific recombinase (NCBI ptt file), at 3,396,070 to 3,396,951 BT2742 Position (kb) 3395 3396 3397Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3395.763 kb on - strandat 3395.763 kb on - strandat 3395.838 kb on - strand, within BT2741at 3395.849 kb on + strand, within BT2741at 3395.850 kb on - strand, within BT2741at 3395.921 kb on + strand, within BT2741at 3395.921 kb on + strand, within BT2741at 3395.944 kb on - strand, within BT2741at 3395.944 kb on - strandat 3396.045 kb on - strandat 3396.054 kb on - strandat 3396.054 kb on - strandat 3396.058 kb on - strandat 3396.058 kb on - strandat 3396.223 kb on - strand, within BT2742

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
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3,395,763 - -0.5
3,395,763 - +1.8
3,395,838 - BT2741 0.28 +0.6
3,395,849 + BT2741 0.32 -2.0
3,395,850 - BT2741 0.32 +2.4
3,395,921 + BT2741 0.56 +2.6
3,395,921 + BT2741 0.56 -0.6
3,395,944 - BT2741 0.63 +4.4
3,395,944 - +1.0
3,396,045 - +0.7
3,396,054 - +2.4
3,396,054 - +2.8
3,396,058 - +2.9
3,396,058 - -2.0
3,396,223 - BT2742 0.17 +1.2

Or see this region's nucleotide sequence