Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2573 and BT2574 are separated by 66 nucleotides BT2574 and tRNA-Phe are separated by 205 nucleotides tRNA-Phe and BT2575 are separated by 234 nucleotides BT2575 and BT2576 overlap by 4 nucleotides
BT2573: BT2573 - glutamate/gamma-aminobutyrate anti-porter (NCBI ptt file), at 3,211,163 to 3,212,875
BT2573
BT2574: BT2574 - two-component system sensor histidine kinase (NCBI ptt file), at 3,212,942 to 3,214,201
BT2574
BT_t40: tRNA-Phe - tRNA-Phe (RefSeq), at 3,214,407 to 3,214,482
tRNA-Phe
BT2575: BT2575 - hypothetical protein (NCBI ptt file), at 3,214,717 to 3,214,938
BT2575
BT2576: BT2576 - transposase (NCBI ptt file), at 3,214,935 to 3,216,170
BT2576
Position (kb)
3212
3213
3214
3215 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3211.949 kb on - strand, within BT2573 at 3211.955 kb on - strand, within BT2573 at 3211.955 kb on - strand, within BT2573 at 3211.958 kb on - strand, within BT2573 at 3211.958 kb on - strand, within BT2573 at 3211.963 kb on - strand, within BT2573 at 3211.991 kb on - strand, within BT2573 at 3211.995 kb on - strand, within BT2573 at 3211.995 kb on - strand, within BT2573 at 3212.116 kb on + strand, within BT2573 at 3212.117 kb on - strand, within BT2573 at 3212.117 kb on - strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.126 kb on + strand, within BT2573 at 3212.128 kb on + strand, within BT2573 at 3212.128 kb on + strand, within BT2573 at 3212.129 kb on - strand, within BT2573 at 3212.129 kb on - strand, within BT2573 at 3212.285 kb on + strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.294 kb on - strand, within BT2573 at 3212.333 kb on + strand, within BT2573 at 3212.333 kb on + strand, within BT2573 at 3212.335 kb on + strand, within BT2573 at 3212.335 kb on + strand, within BT2573 at 3212.524 kb on - strand, within BT2573 at 3212.546 kb on + strand, within BT2573 at 3212.547 kb on - strand, within BT2573 at 3212.547 kb on - strand, within BT2573 at 3212.571 kb on + strand, within BT2573 at 3212.626 kb on + strand, within BT2573 at 3212.638 kb on + strand, within BT2573 at 3212.729 kb on - strand at 3212.762 kb on - strand at 3212.762 kb on - strand at 3212.849 kb on - strand at 3212.886 kb on + strand at 3212.887 kb on - strand at 3212.893 kb on + strand at 3212.893 kb on + strand at 3212.894 kb on - strand at 3212.894 kb on - strand at 3212.897 kb on + strand at 3212.939 kb on + strand at 3212.940 kb on - strand at 3212.945 kb on + strand at 3212.950 kb on + strand at 3212.950 kb on + strand at 3212.961 kb on - strand at 3213.001 kb on + strand at 3213.026 kb on + strand at 3213.050 kb on - strand at 3213.177 kb on + strand, within BT2574 at 3213.177 kb on + strand, within BT2574 at 3213.183 kb on + strand, within BT2574 at 3213.253 kb on + strand, within BT2574 at 3213.272 kb on + strand, within BT2574 at 3213.283 kb on + strand, within BT2574 at 3213.497 kb on - strand, within BT2574 at 3213.508 kb on + strand, within BT2574 at 3213.519 kb on + strand, within BT2574 at 3213.520 kb on - strand, within BT2574 at 3213.520 kb on - strand, within BT2574 at 3213.610 kb on + strand, within BT2574 at 3213.685 kb on + strand, within BT2574 at 3213.709 kb on - strand, within BT2574 at 3213.709 kb on - strand, within BT2574 at 3213.724 kb on + strand, within BT2574 at 3213.750 kb on + strand, within BT2574 at 3213.751 kb on - strand, within BT2574 at 3213.814 kb on + strand, within BT2574 at 3213.863 kb on + strand, within BT2574 at 3213.865 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.871 kb on + strand, within BT2574 at 3213.872 kb on - strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.875 kb on + strand, within BT2574 at 3213.876 kb on - strand, within BT2574 at 3213.878 kb on - strand, within BT2574 at 3213.878 kb on - strand, within BT2574 at 3213.890 kb on - strand, within BT2574 at 3213.997 kb on + strand, within BT2574 at 3213.999 kb on + strand, within BT2574 at 3213.999 kb on + strand, within BT2574 at 3214.003 kb on - strand, within BT2574 at 3214.045 kb on - strand, within BT2574 at 3214.067 kb on + strand, within BT2574 at 3214.068 kb on - strand, within BT2574 at 3214.100 kb on + strand at 3214.101 kb on - strand at 3214.144 kb on + strand at 3214.145 kb on - strand at 3214.145 kb on - strand at 3214.172 kb on + strand at 3214.204 kb on + strand at 3214.204 kb on + strand at 3214.204 kb on + strand at 3214.209 kb on + strand at 3214.210 kb on - strand at 3214.214 kb on + strand at 3214.215 kb on - strand at 3214.279 kb on + strand at 3214.279 kb on + strand at 3214.280 kb on - strand at 3214.354 kb on - strand at 3214.383 kb on - strand at 3214.563 kb on - strand at 3214.652 kb on + strand at 3214.715 kb on + strand at 3214.716 kb on - strand at 3214.769 kb on - strand, within BT2575 at 3214.797 kb on + strand, within BT2575 at 3214.908 kb on + strand, within BT2575 at 3215.006 kb on + strand at 3215.007 kb on - strand at 3215.038 kb on - strand at 3215.100 kb on - strand, within BT2576 at 3215.102 kb on + strand, within BT2576 at 3215.103 kb on - strand, within BT2576 at 3215.139 kb on + strand, within BT2576 at 3215.143 kb on + strand, within BT2576 at 3215.186 kb on + strand, within BT2576 at 3215.186 kb on + strand, within BT2576 at 3215.189 kb on - strand, within BT2576
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 3,211,949 - BT2573 0.46 -0.8 3,211,955 - BT2573 0.46 +0.6 3,211,955 - BT2573 0.46 -0.9 3,211,958 - BT2573 0.46 -2.0 3,211,958 - BT2573 0.46 -3.1 3,211,963 - BT2573 0.47 -1.5 3,211,991 - BT2573 0.48 -2.0 3,211,995 - BT2573 0.49 -2.6 3,211,995 - BT2573 0.49 -1.0 3,212,116 + BT2573 0.56 -3.1 3,212,117 - BT2573 0.56 -2.6 3,212,117 - BT2573 0.56 -1.5 3,212,126 + BT2573 0.56 -3.5 3,212,126 + BT2573 0.56 -2.0 3,212,126 + BT2573 0.56 -2.8 3,212,128 + BT2573 0.56 -1.0 3,212,128 + BT2573 0.56 -2.6 3,212,129 - BT2573 0.56 +0.6 3,212,129 - BT2573 0.56 -1.6 3,212,285 + BT2573 0.65 -2.6 3,212,294 - BT2573 0.66 -3.2 3,212,294 - BT2573 0.66 +1.0 3,212,294 - BT2573 0.66 -1.7 3,212,333 + BT2573 0.68 -3.0 3,212,333 + BT2573 0.68 -1.1 3,212,335 + BT2573 0.68 +0.8 3,212,335 + BT2573 0.68 +1.7 3,212,524 - BT2573 0.79 -2.6 3,212,546 + BT2573 0.81 +1.0 3,212,547 - BT2573 0.81 -1.4 3,212,547 - BT2573 0.81 -1.4 3,212,571 + BT2573 0.82 -0.3 3,212,626 + BT2573 0.85 -2.0 3,212,638 + BT2573 0.86 -1.0 3,212,729 - -0.3 3,212,762 - -3.7 3,212,762 - -0.0 3,212,849 - -0.0 3,212,886 + -3.0 3,212,887 - -2.0 3,212,893 + -1.6 3,212,893 + -0.0 3,212,894 - -3.5 3,212,894 - -1.6 3,212,897 + -3.1 3,212,939 + -1.5 3,212,940 - -1.4 3,212,945 + -0.0 3,212,950 + -0.8 3,212,950 + -0.4 3,212,961 - -2.0 3,213,001 + -1.6 3,213,026 + -2.0 3,213,050 - -1.0 3,213,177 + BT2574 0.19 -2.8 3,213,177 + BT2574 0.19 -2.3 3,213,183 + BT2574 0.19 -2.4 3,213,253 + BT2574 0.25 -2.0 3,213,272 + BT2574 0.26 +2.0 3,213,283 + BT2574 0.27 -1.0 3,213,497 - BT2574 0.44 -0.0 3,213,508 + BT2574 0.45 -0.0 3,213,519 + BT2574 0.46 -3.5 3,213,520 - BT2574 0.46 -0.0 3,213,520 - BT2574 0.46 -2.6 3,213,610 + BT2574 0.53 -0.0 3,213,685 + BT2574 0.59 -0.0 3,213,709 - BT2574 0.61 -3.7 3,213,709 - BT2574 0.61 -2.9 3,213,724 + BT2574 0.62 -2.0 3,213,750 + BT2574 0.64 -1.0 3,213,751 - BT2574 0.64 -1.4 3,213,814 + BT2574 0.69 -1.4 3,213,863 + BT2574 0.73 -3.8 3,213,865 + BT2574 0.73 -3.6 3,213,871 + BT2574 0.74 -1.6 3,213,871 + BT2574 0.74 -2.8 3,213,871 + BT2574 0.74 -1.0 3,213,872 - BT2574 0.74 -0.0 3,213,875 + BT2574 0.74 -2.2 3,213,875 + BT2574 0.74 -0.0 3,213,875 + BT2574 0.74 -1.6 3,213,876 - BT2574 0.74 -0.3 3,213,878 - BT2574 0.74 -2.0 3,213,878 - BT2574 0.74 -2.9 3,213,890 - BT2574 0.75 -1.0 3,213,997 + BT2574 0.84 -0.0 3,213,999 + BT2574 0.84 -0.4 3,213,999 + BT2574 0.84 -4.0 3,214,003 - BT2574 0.84 -1.3 3,214,045 - BT2574 0.88 -0.6 3,214,067 + BT2574 0.89 -1.0 3,214,068 - BT2574 0.89 -2.4 3,214,100 + -0.0 3,214,101 - -1.0 3,214,144 + +0.2 3,214,145 - -4.7 3,214,145 - -2.4 3,214,172 + -0.0 3,214,204 + -1.8 3,214,204 + +0.6 3,214,204 + -1.7 3,214,209 + +1.6 3,214,210 - -1.6 3,214,214 + -2.8 3,214,215 - -0.4 3,214,279 + -0.0 3,214,279 + -0.0 3,214,280 - -1.6 3,214,354 - -1.0 3,214,383 - -0.4 3,214,563 - -1.8 3,214,652 + -3.4 3,214,715 + -3.8 3,214,716 - -2.4 3,214,769 - BT2575 0.23 +2.0 3,214,797 + BT2575 0.36 -1.3 3,214,908 + BT2575 0.86 -1.0 3,215,006 + -2.3 3,215,007 - -2.6 3,215,038 - -1.0 3,215,100 - BT2576 0.13 -0.0 3,215,102 + BT2576 0.14 -0.0 3,215,103 - BT2576 0.14 +0.6 3,215,139 + BT2576 0.17 +0.8 3,215,143 + BT2576 0.17 -1.8 3,215,186 + BT2576 0.20 -2.1 3,215,186 + BT2576 0.20 -0.0 3,215,189 - BT2576 0.21 -0.4
Or see this region's nucleotide sequence