Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2324 and BT2325 are separated by 38 nucleotides BT2325 and BT2326 overlap by 11 nucleotides
BT2324: BT2324 - DNA topoisomerase III (NCBI ptt file), at 2,900,707 to 2,902,806
BT2324
BT2325: BT2325 - hypothetical protein (NCBI ptt file), at 2,902,845 to 2,903,312
BT2325
BT2326: BT2326 - putative DNA methylase (NCBI ptt file), at 2,903,302 to 2,909,028
BT2326
Position (kb)
2902
2903
2904 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2901.867 kb on + strand, within BT2324 at 2902.132 kb on - strand, within BT2324 at 2902.274 kb on - strand, within BT2324 at 2902.275 kb on + strand, within BT2324 at 2902.278 kb on - strand, within BT2324 at 2902.332 kb on + strand, within BT2324 at 2902.400 kb on + strand, within BT2324 at 2902.442 kb on + strand, within BT2324 at 2902.450 kb on + strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.451 kb on - strand, within BT2324 at 2902.732 kb on - strand at 2902.743 kb on + strand at 2902.743 kb on + strand at 2902.744 kb on - strand at 2902.749 kb on - strand at 2902.749 kb on - strand at 2902.805 kb on - strand at 2902.828 kb on - strand at 2902.843 kb on - strand at 2902.856 kb on + strand at 2902.900 kb on + strand, within BT2325 at 2902.905 kb on + strand, within BT2325 at 2902.905 kb on + strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2902.906 kb on - strand, within BT2325 at 2903.284 kb on + strand at 2903.310 kb on + strand at 2903.313 kb on + strand at 2903.338 kb on - strand at 2903.338 kb on - strand at 2903.339 kb on + strand at 2903.339 kb on + strand at 2903.409 kb on - strand at 2903.477 kb on + strand at 2903.570 kb on - strand at 2903.921 kb on + strand, within BT2326 at 2903.922 kb on - strand, within BT2326 at 2903.922 kb on - strand, within BT2326 at 2903.990 kb on - strand, within BT2326 at 2904.225 kb on + strand, within BT2326 at 2904.226 kb on - strand, within BT2326 at 2904.301 kb on - strand, within BT2326
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,901,867 + BT2324 0.55 -1.7 2,902,132 - BT2324 0.68 -2.3 2,902,274 - BT2324 0.75 -0.9 2,902,275 + BT2324 0.75 +0.1 2,902,278 - BT2324 0.75 +2.0 2,902,332 + BT2324 0.77 -1.9 2,902,400 + BT2324 0.81 +1.6 2,902,442 + BT2324 0.83 -2.1 2,902,450 + BT2324 0.83 -1.0 2,902,451 - BT2324 0.83 +1.3 2,902,451 - BT2324 0.83 -0.0 2,902,451 - BT2324 0.83 -2.0 2,902,732 - +0.6 2,902,743 + -1.6 2,902,743 + -0.9 2,902,744 - -1.3 2,902,749 - -0.5 2,902,749 - -0.9 2,902,805 - -2.0 2,902,828 - +1.0 2,902,843 - -2.2 2,902,856 + -0.2 2,902,900 + BT2325 0.12 -0.8 2,902,905 + BT2325 0.13 +0.2 2,902,905 + BT2325 0.13 -3.2 2,902,906 - BT2325 0.13 -0.3 2,902,906 - BT2325 0.13 -0.1 2,902,906 - BT2325 0.13 +0.3 2,903,284 + +0.0 2,903,310 + -1.1 2,903,313 + -3.0 2,903,338 - -1.3 2,903,338 - -2.6 2,903,339 + -3.0 2,903,339 + -0.0 2,903,409 - -1.4 2,903,477 + -1.6 2,903,570 - -0.4 2,903,921 + BT2326 0.11 +2.8 2,903,922 - BT2326 0.11 -3.5 2,903,922 - BT2326 0.11 -2.3 2,903,990 - BT2326 0.12 -2.0 2,904,225 + BT2326 0.16 -1.6 2,904,226 - BT2326 0.16 -1.2 2,904,301 - BT2326 0.17 -1.0
Or see this region's nucleotide sequence