Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1979 and BT1980 are separated by 146 nucleotides BT1980 and BT1981 are separated by 111 nucleotides BT1981 and BT1982 overlap by 4 nucleotides BT1982 and BT1983 are separated by 2 nucleotides
BT1979: BT1979 - Meso-diaminopimelate D-dehydrogenase (NCBI ptt file), at 2,489,425 to 2,490,324
BT1979
BT1980: BT1980 - transposase (NCBI ptt file), at 2,490,471 to 2,492,132
BT1980
BT1981: BT1981 - hypothetical protein (NCBI ptt file), at 2,492,244 to 2,492,549
BT1981
BT1982: BT1982 - hypothetical protein (NCBI ptt file), at 2,492,546 to 2,492,875
BT1982
BT1983: BT1983 - hemolysin III (NCBI ptt file), at 2,492,878 to 2,493,567
BT1983
Position (kb)
2490
2491
2492
2493 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2489.473 kb on + strand at 2489.943 kb on + strand, within BT1979 at 2489.973 kb on - strand, within BT1979 at 2489.973 kb on - strand, within BT1979 at 2489.974 kb on + strand, within BT1979 at 2489.974 kb on + strand at 2489.974 kb on + strand, within BT1979 at 2489.975 kb on - strand, within BT1979 at 2489.976 kb on + strand, within BT1979 at 2490.037 kb on + strand, within BT1979 at 2490.038 kb on - strand, within BT1979 at 2490.038 kb on - strand, within BT1979 at 2490.204 kb on - strand, within BT1979 at 2490.250 kb on + strand at 2490.260 kb on + strand at 2490.260 kb on + strand at 2490.321 kb on - strand at 2490.323 kb on - strand at 2490.427 kb on + strand at 2490.431 kb on - strand at 2490.481 kb on + strand at 2490.568 kb on + strand at 2490.569 kb on - strand at 2490.741 kb on + strand, within BT1980 at 2490.743 kb on + strand, within BT1980 at 2490.818 kb on - strand, within BT1980 at 2490.818 kb on - strand, within BT1980 at 2490.826 kb on + strand, within BT1980 at 2490.827 kb on - strand, within BT1980 at 2490.881 kb on - strand, within BT1980 at 2490.888 kb on - strand, within BT1980 at 2491.186 kb on - strand, within BT1980 at 2491.325 kb on + strand, within BT1980 at 2491.337 kb on - strand, within BT1980 at 2491.434 kb on + strand, within BT1980 at 2491.533 kb on + strand, within BT1980 at 2491.534 kb on - strand, within BT1980 at 2491.539 kb on + strand, within BT1980 at 2491.603 kb on - strand, within BT1980 at 2491.634 kb on + strand, within BT1980 at 2491.655 kb on + strand, within BT1980 at 2491.656 kb on - strand, within BT1980 at 2491.712 kb on + strand, within BT1980 at 2491.712 kb on + strand, within BT1980 at 2491.775 kb on + strand, within BT1980 at 2491.775 kb on + strand, within BT1980 at 2491.776 kb on - strand, within BT1980 at 2491.781 kb on - strand, within BT1980 at 2491.985 kb on + strand at 2492.014 kb on + strand at 2492.045 kb on + strand at 2492.051 kb on + strand at 2492.057 kb on + strand at 2492.107 kb on + strand at 2492.108 kb on + strand at 2492.153 kb on - strand at 2492.159 kb on + strand at 2492.427 kb on + strand, within BT1981 at 2492.427 kb on + strand, within BT1981 at 2492.428 kb on - strand, within BT1981 at 2492.431 kb on - strand, within BT1981 at 2492.431 kb on - strand, within BT1981 at 2492.482 kb on + strand, within BT1981 at 2492.537 kb on - strand at 2492.553 kb on + strand at 2492.604 kb on + strand, within BT1982 at 2492.607 kb on + strand, within BT1982 at 2492.724 kb on + strand, within BT1982 at 2492.726 kb on + strand, within BT1982 at 2492.727 kb on - strand, within BT1982 at 2492.937 kb on + strand at 2492.937 kb on + strand at 2492.938 kb on - strand at 2492.979 kb on + strand, within BT1983 at 2492.982 kb on - strand, within BT1983 at 2493.062 kb on + strand, within BT1983 at 2493.094 kb on + strand, within BT1983 at 2493.113 kb on - strand, within BT1983
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,489,473 + +0.3 2,489,943 + BT1979 0.58 -2.0 2,489,973 - BT1979 0.61 +2.8 2,489,973 - BT1979 0.61 -1.0 2,489,974 + BT1979 0.61 +2.4 2,489,974 + -2.0 2,489,974 + BT1979 0.61 -1.0 2,489,975 - BT1979 0.61 +0.3 2,489,976 + BT1979 0.61 +1.6 2,490,037 + BT1979 0.68 +1.3 2,490,038 - BT1979 0.68 -0.0 2,490,038 - BT1979 0.68 -0.6 2,490,204 - BT1979 0.87 -1.6 2,490,250 + -0.6 2,490,260 + -1.6 2,490,260 + -0.1 2,490,321 - +0.6 2,490,323 - -0.0 2,490,427 + -0.0 2,490,431 - -1.2 2,490,481 + -2.6 2,490,568 + -3.2 2,490,569 - -0.8 2,490,741 + BT1980 0.16 +3.0 2,490,743 + BT1980 0.16 -0.0 2,490,818 - BT1980 0.21 +1.0 2,490,818 - BT1980 0.21 -0.0 2,490,826 + BT1980 0.21 -0.0 2,490,827 - BT1980 0.21 -1.0 2,490,881 - BT1980 0.25 -0.3 2,490,888 - BT1980 0.25 -1.8 2,491,186 - BT1980 0.43 -2.3 2,491,325 + BT1980 0.51 -1.9 2,491,337 - BT1980 0.52 +1.0 2,491,434 + BT1980 0.58 -2.2 2,491,533 + BT1980 0.64 -0.0 2,491,534 - BT1980 0.64 -1.0 2,491,539 + BT1980 0.64 -1.6 2,491,603 - BT1980 0.68 -1.0 2,491,634 + BT1980 0.70 -0.0 2,491,655 + BT1980 0.71 -1.6 2,491,656 - BT1980 0.71 -1.6 2,491,712 + BT1980 0.75 +0.4 2,491,712 + BT1980 0.75 -1.0 2,491,775 + BT1980 0.78 -1.0 2,491,775 + BT1980 0.78 -0.0 2,491,776 - BT1980 0.79 +2.7 2,491,781 - BT1980 0.79 -4.1 2,491,985 + -0.0 2,492,014 + -0.0 2,492,045 + -3.0 2,492,051 + -3.2 2,492,057 + -0.0 2,492,107 + -2.4 2,492,108 + -1.0 2,492,153 - -2.2 2,492,159 + -0.6 2,492,427 + BT1981 0.60 -0.7 2,492,427 + BT1981 0.60 +1.0 2,492,428 - BT1981 0.60 -0.0 2,492,431 - BT1981 0.61 +0.1 2,492,431 - BT1981 0.61 -2.0 2,492,482 + BT1981 0.78 +0.9 2,492,537 - -1.0 2,492,553 + -1.0 2,492,604 + BT1982 0.18 -1.3 2,492,607 + BT1982 0.18 -2.7 2,492,724 + BT1982 0.54 -3.3 2,492,726 + BT1982 0.55 -0.4 2,492,727 - BT1982 0.55 -2.0 2,492,937 + -3.2 2,492,937 + -2.3 2,492,938 - -2.3 2,492,979 + BT1983 0.15 -3.9 2,492,982 - BT1983 0.15 -1.8 2,493,062 + BT1983 0.27 -1.4 2,493,094 + BT1983 0.31 -2.3 2,493,113 - BT1983 0.34 -1.6
Or see this region's nucleotide sequence