Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1815 and BT1816 are separated by 52 nucleotides BT1816 and BT1817 are separated by 10 nucleotides BT1817 and BT1818 are separated by 155 nucleotides
BT1815: BT1815 - low-specificity L-threonine aldolase (NCBI ptt file), at 2,277,969 to 2,278,982
BT1815
BT1816: BT1816 - hypothetical protein (NCBI ptt file), at 2,279,035 to 2,279,409
BT1816
BT1817: BT1817 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,279,420 to 2,279,968
BT1817
BT1818: BT1818 - hypothetical protein (NCBI ptt file), at 2,280,124 to 2,280,540
BT1818
Position (kb)
2279
2280 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2278.097 kb on + strand, within BT1815 at 2278.247 kb on - strand, within BT1815 at 2278.250 kb on + strand, within BT1815 at 2278.254 kb on - strand, within BT1815 at 2278.395 kb on - strand, within BT1815 at 2278.395 kb on - strand, within BT1815 at 2278.416 kb on + strand, within BT1815 at 2278.489 kb on + strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.490 kb on - strand, within BT1815 at 2278.723 kb on + strand, within BT1815 at 2278.723 kb on + strand, within BT1815 at 2278.838 kb on + strand, within BT1815 at 2278.852 kb on - strand, within BT1815 at 2279.012 kb on - strand at 2279.037 kb on - strand at 2279.184 kb on - strand, within BT1816 at 2279.422 kb on - strand at 2279.665 kb on + strand, within BT1817 at 2279.682 kb on - strand, within BT1817 at 2279.733 kb on + strand, within BT1817 at 2279.861 kb on + strand, within BT1817 at 2279.870 kb on - strand, within BT1817 at 2279.870 kb on - strand, within BT1817 at 2279.911 kb on - strand, within BT1817 at 2279.942 kb on - strand at 2279.944 kb on - strand at 2279.944 kb on - strand at 2280.034 kb on + strand at 2280.055 kb on + strand at 2280.123 kb on + strand at 2280.292 kb on + strand, within BT1818 at 2280.356 kb on + strand, within BT1818
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,278,097 + BT1815 0.13 -1.6 2,278,247 - BT1815 0.27 -1.7 2,278,250 + BT1815 0.28 -3.7 2,278,254 - BT1815 0.28 +0.0 2,278,395 - BT1815 0.42 +0.0 2,278,395 - BT1815 0.42 -4.0 2,278,416 + BT1815 0.44 -2.6 2,278,489 + BT1815 0.51 -1.0 2,278,490 - BT1815 0.51 -2.3 2,278,490 - BT1815 0.51 -2.0 2,278,490 - BT1815 0.51 -2.5 2,278,490 - BT1815 0.51 +0.0 2,278,723 + BT1815 0.74 -1.6 2,278,723 + BT1815 0.74 -4.3 2,278,838 + BT1815 0.86 -1.0 2,278,852 - BT1815 0.87 +0.0 2,279,012 - -0.7 2,279,037 - -0.7 2,279,184 - BT1816 0.40 -2.6 2,279,422 - -1.0 2,279,665 + BT1817 0.45 +0.9 2,279,682 - BT1817 0.48 -1.6 2,279,733 + BT1817 0.57 +0.9 2,279,861 + BT1817 0.80 -2.6 2,279,870 - BT1817 0.82 +0.0 2,279,870 - BT1817 0.82 -1.0 2,279,911 - BT1817 0.89 -1.0 2,279,942 - -2.8 2,279,944 - -4.4 2,279,944 - -1.0 2,280,034 + +2.0 2,280,055 + -2.0 2,280,123 + +0.0 2,280,292 + BT1818 0.40 +0.0 2,280,356 + BT1818 0.56 +0.0
Or see this region's nucleotide sequence