Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0541 and BT0542 are separated by 172 nucleotides BT0542 and BT0543 are separated by 121 nucleotides
BT0541: BT0541 - Polyphosphate kinase (NCBI ptt file), at 664,997 to 667,114
BT0541
BT0542: BT0542 - conserved hypothetical protein (NCBI ptt file), at 667,287 to 667,865
BT0542
BT0543: BT0543 - glutamine synthetase (NCBI ptt file), at 667,987 to 670,176
BT0543
Position (kb) 667
668 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 666.298 kb on - strand, within BT0541 at 666.359 kb on + strand, within BT0541 at 666.421 kb on - strand, within BT0541 at 666.502 kb on - strand, within BT0541 at 666.533 kb on - strand, within BT0541 at 666.543 kb on + strand, within BT0541 at 666.544 kb on - strand, within BT0541 at 666.635 kb on - strand, within BT0541 at 666.855 kb on - strand, within BT0541 at 666.897 kb on - strand, within BT0541 at 666.935 kb on - strand at 666.976 kb on - strand at 666.976 kb on - strand at 667.011 kb on - strand at 667.220 kb on + strand at 667.228 kb on + strand at 667.239 kb on - strand at 667.284 kb on + strand at 667.302 kb on + strand at 667.377 kb on + strand, within BT0542 at 667.575 kb on - strand, within BT0542 at 667.718 kb on + strand, within BT0542 at 667.718 kb on + strand, within BT0542 at 667.719 kb on - strand, within BT0542 at 667.719 kb on - strand, within BT0542 at 667.839 kb on - strand at 667.858 kb on + strand at 667.947 kb on + strand at 667.947 kb on + strand at 667.964 kb on - strand at 667.994 kb on - strand at 668.065 kb on - strand at 668.065 kb on - strand at 668.101 kb on - strand at 668.166 kb on - strand at 668.176 kb on + strand at 668.292 kb on + strand, within BT0543 at 668.293 kb on - strand, within BT0543 at 668.293 kb on - strand, within BT0543 at 668.293 kb on - strand, within BT0543 at 668.332 kb on - strand, within BT0543 at 668.461 kb on - strand, within BT0543 at 668.483 kb on + strand, within BT0543 at 668.543 kb on - strand, within BT0543 at 668.614 kb on + strand, within BT0543 at 668.811 kb on + strand, within BT0543
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 666,298 - BT0541 0.61 +1.3 666,359 + BT0541 0.64 -1.0 666,421 - BT0541 0.67 -0.0 666,502 - BT0541 0.71 +1.6 666,533 - BT0541 0.73 -1.3 666,543 + BT0541 0.73 -0.0 666,544 - BT0541 0.73 -2.6 666,635 - BT0541 0.77 -1.0 666,855 - BT0541 0.88 -3.0 666,897 - BT0541 0.90 -2.0 666,935 - -1.5 666,976 - +0.0 666,976 - +0.0 667,011 - -1.0 667,220 + -2.3 667,228 + -1.0 667,239 - +0.0 667,284 + -2.8 667,302 + -1.8 667,377 + BT0542 0.16 +2.2 667,575 - BT0542 0.50 -2.3 667,718 + BT0542 0.74 -1.6 667,718 + BT0542 0.74 +0.0 667,719 - BT0542 0.75 +3.6 667,719 - BT0542 0.75 +1.3 667,839 - -2.6 667,858 + -1.0 667,947 + +0.0 667,947 + +1.6 667,964 - -1.0 667,994 - -3.5 668,065 - -4.8 668,065 - -2.0 668,101 - -3.2 668,166 - -3.5 668,176 + -0.6 668,292 + BT0543 0.14 +0.0 668,293 - BT0543 0.14 -1.3 668,293 - BT0543 0.14 -0.6 668,293 - BT0543 0.14 -2.3 668,332 - BT0543 0.16 +0.0 668,461 - BT0543 0.22 -1.0 668,483 + BT0543 0.23 +2.9 668,543 - BT0543 0.25 -1.6 668,614 + BT0543 0.29 -1.6 668,811 + BT0543 0.38 -2.0
Or see this region's nucleotide sequence