Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0046

Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0045 and BT0046 are separated by 23 nucleotidesBT0046 and BT0047 are separated by 14 nucleotidesBT0047 and BT0048 are separated by 95 nucleotidesBT0048 and BT0049 are separated by 8 nucleotides BT0045: BT0045 - putative polysaccharide biosynthesis protein (NCBI ptt file), at 41,534 to 42,700 BT0045 BT0046: BT0046 - putative glycosyltransferase (NCBI ptt file), at 42,724 to 43,656 BT0046 BT0047: BT0047 - putative glycosyltransferase (NCBI ptt file), at 43,671 to 44,066 BT0047 BT0048: BT0048 - hypothetical protein (NCBI ptt file), at 44,162 to 44,638 BT0048 BT0049: BT0049 - putative glycosyltransferase (NCBI ptt file), at 44,647 to 45,582 BT0049 Position (kb) 42 43 44Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 41.738 kb on + strand, within BT0045at 41.785 kb on + strand, within BT0045at 41.836 kb on + strand, within BT0045at 41.852 kb on + strand, within BT0045at 41.870 kb on - strand, within BT0045at 41.872 kb on - strand, within BT0045at 41.879 kb on - strand, within BT0045at 41.879 kb on - strand, within BT0045at 41.921 kb on - strand, within BT0045at 41.936 kb on - strand, within BT0045at 41.943 kb on + strand, within BT0045at 41.944 kb on - strand, within BT0045at 41.944 kb on - strand, within BT0045at 41.979 kb on - strand, within BT0045at 41.984 kb on + strand, within BT0045at 41.984 kb on + strand, within BT0045at 41.984 kb on + strand, within BT0045at 41.985 kb on - strand, within BT0045at 41.985 kb on - strand, within BT0045at 41.985 kb on - strand, within BT0045at 41.999 kb on - strand, within BT0045at 42.048 kb on - strand, within BT0045at 42.058 kb on + strand, within BT0045at 42.074 kb on + strand, within BT0045at 42.172 kb on - strand, within BT0045at 42.178 kb on - strand, within BT0045at 42.197 kb on - strand, within BT0045at 42.289 kb on - strand, within BT0045at 42.345 kb on - strand, within BT0045at 42.365 kb on + strand, within BT0045at 42.365 kb on + strand, within BT0045at 42.366 kb on - strand, within BT0045at 42.367 kb on + strand, within BT0045at 42.367 kb on + strand, within BT0045at 42.368 kb on - strand, within BT0045at 42.368 kb on - strand, within BT0045at 42.370 kb on + strand, within BT0045at 42.400 kb on + strand, within BT0045at 42.503 kb on - strand, within BT0045at 42.506 kb on + strand, within BT0045at 42.572 kb on - strand, within BT0045at 42.589 kb on + strandat 42.599 kb on - strandat 42.625 kb on - strandat 42.659 kb on + strandat 42.682 kb on - strandat 42.757 kb on - strandat 42.759 kb on + strandat 42.769 kb on + strandat 42.770 kb on - strandat 42.846 kb on + strand, within BT0046at 42.878 kb on - strand, within BT0046at 42.906 kb on - strand, within BT0046at 42.907 kb on + strand, within BT0046at 42.908 kb on - strand, within BT0046at 43.161 kb on + strand, within BT0046at 43.197 kb on - strand, within BT0046at 43.197 kb on - strand, within BT0046at 43.278 kb on - strand, within BT0046at 43.325 kb on + strand, within BT0046at 43.325 kb on + strand, within BT0046at 43.326 kb on - strand, within BT0046at 43.328 kb on - strand, within BT0046at 43.341 kb on - strand, within BT0046at 43.605 kb on - strandat 43.681 kb on + strandat 43.701 kb on + strandat 43.755 kb on - strand, within BT0047at 43.774 kb on + strand, within BT0047at 43.843 kb on - strand, within BT0047at 43.858 kb on - strand, within BT0047at 43.899 kb on + strand, within BT0047at 43.899 kb on + strand, within BT0047at 43.899 kb on + strand, within BT0047at 43.957 kb on - strand, within BT0047at 44.167 kb on + strandat 44.180 kb on - strandat 44.266 kb on - strand, within BT0048at 44.315 kb on - strand, within BT0048at 44.353 kb on - strand, within BT0048at 44.376 kb on - strand, within BT0048at 44.376 kb on - strand, within BT0048at 44.409 kb on + strand, within BT0048at 44.468 kb on + strand, within BT0048at 44.510 kb on - strand, within BT0048at 44.600 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
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41,738 + BT0045 0.17 -1.9
41,785 + BT0045 0.22 -0.0
41,836 + BT0045 0.26 -2.9
41,852 + BT0045 0.27 +2.0
41,870 - BT0045 0.29 -2.6
41,872 - BT0045 0.29 -1.0
41,879 - BT0045 0.30 -1.6
41,879 - BT0045 0.30 -2.0
41,921 - BT0045 0.33 +0.3
41,936 - BT0045 0.34 -2.3
41,943 + BT0045 0.35 -1.0
41,944 - BT0045 0.35 -1.0
41,944 - BT0045 0.35 -1.6
41,979 - BT0045 0.38 +1.0
41,984 + BT0045 0.39 -0.0
41,984 + BT0045 0.39 -1.0
41,984 + BT0045 0.39 -0.8
41,985 - BT0045 0.39 -1.6
41,985 - BT0045 0.39 -1.6
41,985 - BT0045 0.39 -1.8
41,999 - BT0045 0.40 +1.6
42,048 - BT0045 0.44 -2.0
42,058 + BT0045 0.45 -0.0
42,074 + BT0045 0.46 -1.0
42,172 - BT0045 0.55 -1.8
42,178 - BT0045 0.55 +0.6
42,197 - BT0045 0.57 -3.0
42,289 - BT0045 0.65 -1.3
42,345 - BT0045 0.69 -1.6
42,365 + BT0045 0.71 -2.6
42,365 + BT0045 0.71 -1.0
42,366 - BT0045 0.71 -0.0
42,367 + BT0045 0.71 -2.3
42,367 + BT0045 0.71 -1.0
42,368 - BT0045 0.71 -0.2
42,368 - BT0045 0.71 +2.3
42,370 + BT0045 0.72 -1.4
42,400 + BT0045 0.74 -2.0
42,503 - BT0045 0.83 -1.0
42,506 + BT0045 0.83 -2.0
42,572 - BT0045 0.89 -1.0
42,589 + -3.6
42,599 - -3.9
42,625 - -0.0
42,659 + -2.8
42,682 - -2.3
42,757 - +0.2
42,759 + -2.3
42,769 + -1.0
42,770 - +0.5
42,846 + BT0046 0.13 -2.2
42,878 - BT0046 0.17 -1.6
42,906 - BT0046 0.20 -3.7
42,907 + BT0046 0.20 -2.3
42,908 - BT0046 0.20 -0.0
43,161 + BT0046 0.47 -2.0
43,197 - BT0046 0.51 -1.0
43,197 - BT0046 0.51 -2.0
43,278 - BT0046 0.59 -1.6
43,325 + BT0046 0.64 -4.1
43,325 + BT0046 0.64 -1.0
43,326 - BT0046 0.65 -3.0
43,328 - BT0046 0.65 -2.8
43,341 - BT0046 0.66 -0.8
43,605 - +0.6
43,681 + -2.3
43,701 + +1.0
43,755 - BT0047 0.21 +2.1
43,774 + BT0047 0.26 -3.8
43,843 - BT0047 0.43 -2.9
43,858 - BT0047 0.47 -1.6
43,899 + BT0047 0.58 -1.6
43,899 + BT0047 0.58 -0.0
43,899 + BT0047 0.58 -4.0
43,957 - BT0047 0.72 -1.8
44,167 + -2.6
44,180 - -3.0
44,266 - BT0048 0.22 -2.6
44,315 - BT0048 0.32 -1.0
44,353 - BT0048 0.40 -0.4
44,376 - BT0048 0.45 +3.2
44,376 - BT0048 0.45 -2.6
44,409 + BT0048 0.52 -3.6
44,468 + BT0048 0.64 -1.0
44,510 - BT0048 0.73 -0.0
44,600 - -1.6

Or see this region's nucleotide sequence