Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2066 and BT2067 overlap by 4 nucleotides BT2067 and BT2068 are separated by 38 nucleotides
BT2066: BT2066 - putative oxidoreductase (NCBI ptt file), at 2,587,481 to 2,588,332
BT2066
BT2067: BT2067 - putative NADH:flavin oxidoreductase (NCBI ptt file), at 2,588,329 to 2,589,552
BT2067
BT2068: BT2068 - 3-oxo-5-alpha-steroid 4-dehydrogenase (NCBI ptt file), at 2,589,591 to 2,590,352
BT2068
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2587.346 kb on + strand at 2587.432 kb on - strand at 2587.432 kb on - strand at 2587.462 kb on - strand at 2587.717 kb on + strand, within BT2066 at 2587.805 kb on - strand, within BT2066 at 2587.911 kb on + strand, within BT2066 at 2587.912 kb on - strand, within BT2066 at 2587.912 kb on - strand at 2587.912 kb on - strand, within BT2066 at 2587.931 kb on + strand, within BT2066 at 2587.996 kb on - strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.239 kb on + strand, within BT2066 at 2588.240 kb on - strand, within BT2066 at 2588.242 kb on + strand, within BT2066 at 2588.417 kb on + strand at 2588.417 kb on + strand at 2588.435 kb on + strand at 2588.455 kb on - strand, within BT2067 at 2588.552 kb on - strand, within BT2067 at 2588.649 kb on - strand, within BT2067 at 2588.706 kb on - strand, within BT2067 at 2588.772 kb on - strand, within BT2067 at 2589.020 kb on + strand, within BT2067 at 2589.021 kb on - strand, within BT2067 at 2589.048 kb on - strand, within BT2067 at 2589.064 kb on + strand, within BT2067 at 2589.064 kb on + strand, within BT2067 at 2589.192 kb on + strand, within BT2067 at 2589.237 kb on + strand, within BT2067 at 2589.238 kb on - strand, within BT2067 at 2589.238 kb on - strand, within BT2067 at 2589.380 kb on + strand, within BT2067 at 2589.385 kb on + strand, within BT2067 at 2589.385 kb on + strand, within BT2067 at 2589.385 kb on + strand, within BT2067 at 2589.553 kb on - strand at 2589.553 kb on - strand at 2589.555 kb on + strand at 2589.555 kb on + strand at 2589.556 kb on - strand at 2589.565 kb on + strand at 2589.582 kb on + strand at 2589.595 kb on + strand at 2589.711 kb on + strand, within BT2068 at 2589.711 kb on + strand, within BT2068 at 2589.776 kb on - strand, within BT2068 at 2589.799 kb on + strand, within BT2068 at 2589.800 kb on - strand, within BT2068 at 2589.800 kb on - strand, within BT2068 at 2589.800 kb on - strand, within BT2068 at 2589.820 kb on + strand, within BT2068 at 2589.860 kb on + strand, within BT2068 at 2589.918 kb on - strand, within BT2068 at 2589.918 kb on - strand, within BT2068 at 2589.950 kb on - strand, within BT2068 at 2589.970 kb on + strand, within BT2068 at 2589.976 kb on - strand, within BT2068 at 2589.994 kb on + strand, within BT2068 at 2589.995 kb on - strand, within BT2068 at 2589.995 kb on - strand, within BT2068 at 2590.005 kb on + strand, within BT2068 at 2590.186 kb on - strand, within BT2068 at 2590.186 kb on - strand, within BT2068 at 2590.345 kb on - strand at 2590.347 kb on - strand at 2590.347 kb on - strand at 2590.413 kb on - strand at 2590.466 kb on - strand at 2590.473 kb on - strand at 2590.506 kb on + strand at 2590.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,587,346 + -0.9 2,587,432 - -2.5 2,587,432 - +0.1 2,587,462 - +1.2 2,587,717 + BT2066 0.28 -1.8 2,587,805 - BT2066 0.38 -0.9 2,587,911 + BT2066 0.50 -0.9 2,587,912 - BT2066 0.51 -2.2 2,587,912 - +0.1 2,587,912 - BT2066 0.51 -1.9 2,587,931 + BT2066 0.53 -0.9 2,587,996 - BT2066 0.60 -1.9 2,588,239 + BT2066 0.89 -0.9 2,588,239 + BT2066 0.89 +0.1 2,588,239 + BT2066 0.89 -2.3 2,588,240 - BT2066 0.89 +1.1 2,588,242 + BT2066 0.89 +0.1 2,588,417 + -0.7 2,588,417 + -1.9 2,588,435 + -0.9 2,588,455 - BT2067 0.10 +2.9 2,588,552 - BT2067 0.18 +0.1 2,588,649 - BT2067 0.26 -0.2 2,588,706 - BT2067 0.31 +0.3 2,588,772 - BT2067 0.36 -1.7 2,589,020 + BT2067 0.56 -0.3 2,589,021 - BT2067 0.57 -1.9 2,589,048 - BT2067 0.59 +0.1 2,589,064 + BT2067 0.60 +0.1 2,589,064 + BT2067 0.60 +0.1 2,589,192 + BT2067 0.71 -0.9 2,589,237 + BT2067 0.74 -4.2 2,589,238 - BT2067 0.74 -1.9 2,589,238 - BT2067 0.74 +0.1 2,589,380 + BT2067 0.86 +0.1 2,589,385 + BT2067 0.86 -0.5 2,589,385 + BT2067 0.86 -1.1 2,589,385 + BT2067 0.86 +2.6 2,589,553 - +0.1 2,589,553 - -0.8 2,589,555 + +0.1 2,589,555 + -1.9 2,589,556 - -2.6 2,589,565 + -3.5 2,589,582 + +0.1 2,589,595 + -2.2 2,589,711 + BT2068 0.16 +0.0 2,589,711 + BT2068 0.16 -0.4 2,589,776 - BT2068 0.24 -1.5 2,589,799 + BT2068 0.27 -3.3 2,589,800 - BT2068 0.27 -3.4 2,589,800 - BT2068 0.27 +1.1 2,589,800 - BT2068 0.27 -1.9 2,589,820 + BT2068 0.30 +0.5 2,589,860 + BT2068 0.35 -2.1 2,589,918 - BT2068 0.43 -3.1 2,589,918 - BT2068 0.43 -0.6 2,589,950 - BT2068 0.47 -1.5 2,589,970 + BT2068 0.50 -2.5 2,589,976 - BT2068 0.51 -3.6 2,589,994 + BT2068 0.53 -1.7 2,589,995 - BT2068 0.53 -0.3 2,589,995 - BT2068 0.53 +0.4 2,590,005 + BT2068 0.54 -2.9 2,590,186 - BT2068 0.78 -3.6 2,590,186 - BT2068 0.78 -1.3 2,590,345 - +2.5 2,590,347 - -2.7 2,590,347 - -1.9 2,590,413 - -1.3 2,590,466 - -1.9 2,590,473 - -0.9 2,590,506 + -0.9 2,590,538 + +1.1
Or see this region's nucleotide sequence