Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0142 and BT0143 are separated by 9 nucleotides BT0143 and BT0144 are separated by 204 nucleotides BT0144 and BT0145 are separated by 160 nucleotides
BT0142: BT0142 - conserved hypothetical protein (NCBI ptt file), at 144,992 to 146,377
BT0142
BT0143: BT0143 - putative transmembrane protein (NCBI ptt file), at 146,387 to 147,763
BT0143
BT0144: BT0144 - putative beta-xylosidase (NCBI ptt file), at 147,968 to 148,198
BT0144
BT0145: BT0145 - putative beta-xylosidase (NCBI ptt file), at 148,359 to 149,618
BT0145
Position (kb) 146
147
148 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 145.460 kb on - strand, within BT0142 at 145.596 kb on + strand, within BT0142 at 145.657 kb on + strand, within BT0142 at 145.666 kb on - strand, within BT0142 at 145.666 kb on - strand, within BT0142 at 145.672 kb on + strand, within BT0142 at 145.725 kb on + strand, within BT0142 at 145.891 kb on - strand, within BT0142 at 145.991 kb on - strand, within BT0142 at 145.991 kb on - strand, within BT0142 at 146.018 kb on - strand, within BT0142 at 146.042 kb on - strand, within BT0142 at 146.065 kb on + strand, within BT0142 at 146.066 kb on - strand, within BT0142 at 146.066 kb on - strand, within BT0142 at 146.068 kb on - strand, within BT0142 at 146.110 kb on + strand, within BT0142 at 146.194 kb on - strand, within BT0142 at 146.196 kb on - strand, within BT0142 at 146.198 kb on + strand, within BT0142 at 146.213 kb on - strand, within BT0142 at 146.220 kb on - strand, within BT0142 at 146.303 kb on - strand at 146.303 kb on - strand at 146.307 kb on + strand at 146.307 kb on + strand at 146.307 kb on + strand at 146.309 kb on + strand at 146.310 kb on - strand at 146.310 kb on - strand at 146.428 kb on - strand at 146.479 kb on + strand at 146.512 kb on + strand at 146.512 kb on + strand at 146.513 kb on - strand at 146.661 kb on - strand, within BT0143 at 146.761 kb on + strand, within BT0143 at 146.765 kb on + strand, within BT0143 at 146.897 kb on + strand, within BT0143 at 146.897 kb on + strand, within BT0143 at 146.898 kb on - strand, within BT0143 at 146.938 kb on + strand, within BT0143 at 146.985 kb on - strand, within BT0143 at 146.998 kb on + strand, within BT0143 at 147.095 kb on - strand, within BT0143 at 147.166 kb on + strand, within BT0143 at 147.218 kb on + strand, within BT0143 at 147.235 kb on + strand, within BT0143 at 147.373 kb on + strand, within BT0143 at 147.373 kb on + strand, within BT0143 at 147.431 kb on + strand, within BT0143 at 147.431 kb on + strand, within BT0143 at 147.435 kb on + strand, within BT0143 at 147.527 kb on - strand, within BT0143 at 147.564 kb on + strand, within BT0143 at 147.564 kb on + strand, within BT0143 at 147.569 kb on - strand, within BT0143 at 147.730 kb on - strand at 147.730 kb on - strand at 147.749 kb on + strand at 147.801 kb on - strand at 147.801 kb on - strand at 147.805 kb on - strand at 147.824 kb on + strand at 147.826 kb on + strand at 147.826 kb on + strand at 147.827 kb on - strand at 147.827 kb on - strand at 147.846 kb on - strand at 147.937 kb on + strand at 147.965 kb on - strand at 147.968 kb on - strand at 148.069 kb on + strand, within BT0144 at 148.074 kb on - strand, within BT0144 at 148.117 kb on - strand, within BT0144 at 148.225 kb on - strand at 148.286 kb on - strand at 148.356 kb on + strand at 148.357 kb on - strand at 148.475 kb on - strand at 148.483 kb on - strand at 148.601 kb on + strand, within BT0145 at 148.638 kb on + strand, within BT0145
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 145,460 - BT0142 0.34 -1.0 145,596 + BT0142 0.44 -2.8 145,657 + BT0142 0.48 -1.5 145,666 - BT0142 0.49 +1.6 145,666 - BT0142 0.49 -1.0 145,672 + BT0142 0.49 -1.5 145,725 + BT0142 0.53 -2.3 145,891 - BT0142 0.65 -1.0 145,991 - BT0142 0.72 +2.0 145,991 - BT0142 0.72 -1.1 146,018 - BT0142 0.74 +0.0 146,042 - BT0142 0.76 +0.0 146,065 + BT0142 0.77 -1.5 146,066 - BT0142 0.77 +0.3 146,066 - BT0142 0.77 -2.3 146,068 - BT0142 0.78 -1.4 146,110 + BT0142 0.81 +1.0 146,194 - BT0142 0.87 -1.5 146,196 - BT0142 0.87 +0.0 146,198 + BT0142 0.87 -0.5 146,213 - BT0142 0.88 -0.5 146,220 - BT0142 0.89 +0.0 146,303 - -2.3 146,303 - -2.4 146,307 + +0.0 146,307 + -2.0 146,307 + -3.3 146,309 + +0.0 146,310 - +0.0 146,310 - -1.5 146,428 - +0.9 146,479 + -0.3 146,512 + -2.5 146,512 + +0.0 146,513 - +3.5 146,661 - BT0143 0.20 +3.6 146,761 + BT0143 0.27 +0.0 146,765 + BT0143 0.27 -1.5 146,897 + BT0143 0.37 +2.4 146,897 + BT0143 0.37 -1.2 146,898 - BT0143 0.37 -2.0 146,938 + BT0143 0.40 +0.0 146,985 - BT0143 0.43 +0.0 146,998 + BT0143 0.44 +0.0 147,095 - BT0143 0.51 -1.0 147,166 + BT0143 0.57 -1.0 147,218 + BT0143 0.60 +0.6 147,235 + BT0143 0.62 -1.0 147,373 + BT0143 0.72 -1.2 147,373 + BT0143 0.72 -3.9 147,431 + BT0143 0.76 -2.5 147,431 + BT0143 0.76 +0.6 147,435 + BT0143 0.76 -1.0 147,527 - BT0143 0.83 -2.0 147,564 + BT0143 0.85 -2.0 147,564 + BT0143 0.85 +0.5 147,569 - BT0143 0.86 -1.8 147,730 - -2.0 147,730 - +0.5 147,749 + -2.1 147,801 - -1.6 147,801 - -1.5 147,805 - +0.0 147,824 + -0.8 147,826 + -1.5 147,826 + +0.0 147,827 - -1.2 147,827 - -1.7 147,846 - -2.3 147,937 + -1.5 147,965 - +0.0 147,968 - -1.5 148,069 + BT0144 0.44 -2.0 148,074 - BT0144 0.46 +0.4 148,117 - BT0144 0.65 -3.4 148,225 - +0.0 148,286 - +0.5 148,356 + -1.5 148,357 - -1.3 148,475 - -0.1 148,483 - +0.6 148,601 + BT0145 0.19 -1.0 148,638 + BT0145 0.22 -0.5
Or see this region's nucleotide sequence