Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0051 and BT0052 are separated by 170 nucleotides BT0052 and BT0053 overlap by 1 nucleotides
BT0051: BT0051 - putative glycosyltransferase (NCBI ptt file), at 46,565 to 47,545
BT0051
BT0052: BT0052 - hypothetical protein (NCBI ptt file), at 47,716 to 48,834
BT0052
BT0053: BT0053 - glycosyltransferase (NCBI ptt file), at 48,834 to 49,958
BT0053
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 46.755 kb on + strand, within BT0051 at 46.826 kb on + strand, within BT0051 at 46.916 kb on - strand, within BT0051 at 47.011 kb on - strand, within BT0051 at 47.042 kb on + strand, within BT0051 at 47.043 kb on - strand, within BT0051 at 47.064 kb on - strand, within BT0051 at 47.068 kb on - strand, within BT0051 at 47.103 kb on + strand, within BT0051 at 47.122 kb on - strand, within BT0051 at 47.416 kb on + strand, within BT0051 at 47.512 kb on - strand at 47.559 kb on + strand at 47.565 kb on - strand at 47.565 kb on - strand at 47.565 kb on - strand at 47.592 kb on + strand at 47.631 kb on + strand at 47.635 kb on - strand at 47.642 kb on - strand at 47.642 kb on - strand at 47.794 kb on - strand at 47.800 kb on + strand at 47.800 kb on + strand at 47.809 kb on + strand at 47.809 kb on + strand at 47.810 kb on - strand at 47.810 kb on - strand at 47.816 kb on + strand at 47.817 kb on - strand at 47.817 kb on - strand at 47.862 kb on + strand, within BT0052 at 47.863 kb on - strand, within BT0052 at 47.909 kb on - strand, within BT0052 at 47.909 kb on - strand, within BT0052 at 47.992 kb on + strand, within BT0052 at 48.037 kb on + strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.038 kb on - strand, within BT0052 at 48.099 kb on - strand, within BT0052 at 48.152 kb on + strand, within BT0052 at 48.177 kb on + strand, within BT0052 at 48.186 kb on + strand, within BT0052 at 48.189 kb on + strand, within BT0052 at 48.190 kb on - strand, within BT0052 at 48.222 kb on - strand, within BT0052 at 48.242 kb on + strand, within BT0052 at 48.242 kb on + strand, within BT0052 at 48.243 kb on - strand, within BT0052 at 48.324 kb on + strand, within BT0052 at 48.325 kb on - strand, within BT0052 at 48.366 kb on + strand, within BT0052 at 48.414 kb on + strand, within BT0052 at 48.476 kb on + strand, within BT0052 at 48.477 kb on - strand, within BT0052 at 48.502 kb on + strand, within BT0052 at 48.559 kb on + strand, within BT0052 at 48.611 kb on + strand, within BT0052 at 48.685 kb on - strand, within BT0052 at 48.692 kb on + strand, within BT0052 at 48.693 kb on - strand, within BT0052 at 48.723 kb on - strand at 48.760 kb on + strand at 48.845 kb on - strand at 48.845 kb on - strand at 48.847 kb on - strand at 48.847 kb on - strand at 48.890 kb on - strand at 48.927 kb on + strand at 48.934 kb on + strand at 48.934 kb on + strand at 49.021 kb on - strand, within BT0053 at 49.053 kb on - strand, within BT0053 at 49.088 kb on + strand, within BT0053 at 49.109 kb on - strand, within BT0053 at 49.134 kb on - strand, within BT0053 at 49.134 kb on - strand, within BT0053 at 49.348 kb on - strand, within BT0053 at 49.533 kb on - strand, within BT0053 at 49.609 kb on + strand, within BT0053 at 49.692 kb on - strand, within BT0053 at 49.728 kb on - strand, within BT0053
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 46,755 + BT0051 0.19 -2.3 46,826 + BT0051 0.27 -2.0 46,916 - BT0051 0.36 +0.6 47,011 - BT0051 0.45 -1.5 47,042 + BT0051 0.49 +0.0 47,043 - BT0051 0.49 +1.6 47,064 - BT0051 0.51 -2.5 47,068 - BT0051 0.51 -0.8 47,103 + BT0051 0.55 +0.0 47,122 - BT0051 0.57 -1.0 47,416 + BT0051 0.87 +0.0 47,512 - -1.5 47,559 + -0.4 47,565 - -1.0 47,565 - -2.3 47,565 - -1.0 47,592 + -3.4 47,631 + -1.5 47,635 - -1.8 47,642 - -1.0 47,642 - +0.0 47,794 - -2.8 47,800 + -1.0 47,800 + -1.5 47,809 + -3.7 47,809 + -2.0 47,810 - +0.0 47,810 - -1.5 47,816 + -1.0 47,817 - +0.0 47,817 - -1.5 47,862 + BT0052 0.13 -4.7 47,863 - BT0052 0.13 +0.0 47,909 - BT0052 0.17 -2.0 47,909 - BT0052 0.17 -1.1 47,992 + BT0052 0.25 -0.2 48,037 + BT0052 0.29 -1.5 48,038 - BT0052 0.29 +2.4 48,038 - BT0052 0.29 -3.1 48,038 - BT0052 0.29 -2.3 48,099 - BT0052 0.34 -2.0 48,152 + BT0052 0.39 -3.1 48,177 + BT0052 0.41 +0.6 48,186 + BT0052 0.42 -3.5 48,189 + BT0052 0.42 -2.8 48,190 - BT0052 0.42 -3.4 48,222 - BT0052 0.45 +0.0 48,242 + BT0052 0.47 -3.1 48,242 + BT0052 0.47 +0.0 48,243 - BT0052 0.47 -2.0 48,324 + BT0052 0.54 -1.5 48,325 - BT0052 0.54 -1.1 48,366 + BT0052 0.58 -1.5 48,414 + BT0052 0.62 -1.5 48,476 + BT0052 0.68 +0.0 48,477 - BT0052 0.68 +1.4 48,502 + BT0052 0.70 -1.0 48,559 + BT0052 0.75 -2.0 48,611 + BT0052 0.80 -2.0 48,685 - BT0052 0.87 -1.0 48,692 + BT0052 0.87 -1.5 48,693 - BT0052 0.87 -1.5 48,723 - -2.8 48,760 + -1.5 48,845 - -1.4 48,845 - -3.9 48,847 - -1.0 48,847 - -2.3 48,890 - -2.0 48,927 + -2.0 48,934 + -0.3 48,934 + +0.0 49,021 - BT0053 0.17 -1.0 49,053 - BT0053 0.19 +0.4 49,088 + BT0053 0.23 -2.8 49,109 - BT0053 0.24 -1.4 49,134 - BT0053 0.27 -2.1 49,134 - BT0053 0.27 -1.5 49,348 - BT0053 0.46 +0.8 49,533 - BT0053 0.62 -1.7 49,609 + BT0053 0.69 +0.0 49,692 - BT0053 0.76 -1.2 49,728 - BT0053 0.79 +0.0
Or see this region's nucleotide sequence