Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4214 and BT4215 are separated by 152 nucleotides BT4215 and BT4216 are separated by 38 nucleotides BT4216 and BT4217 are separated by 2 nucleotides
BT4214: BT4214 - conserved hypothetical protein (NCBI ptt file), at 5,551,068 to 5,551,940
BT4214
BT4215: BT4215 - shikimate dehydrogenase (EC 1.1.1.25) <small>(from data)</small>, at 5,552,093 to 5,552,839
BT4215
BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615
BT4216
BT4217: BT4217 - phosphoribosylamidoimidazole-succinocarboxamide synthase (NCBI ptt file), at 5,553,618 to 5,554,562
BT4217
Position (kb) 5552
5553 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5551.111 kb on + strand at 5551.112 kb on - strand at 5551.121 kb on + strand at 5551.225 kb on + strand, within BT4214 at 5551.225 kb on + strand, within BT4214 at 5551.225 kb on + strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.230 kb on - strand, within BT4214 at 5551.230 kb on - strand, within BT4214 at 5551.340 kb on + strand, within BT4214 at 5551.345 kb on + strand, within BT4214 at 5551.407 kb on + strand, within BT4214 at 5551.448 kb on - strand, within BT4214 at 5551.503 kb on - strand, within BT4214 at 5551.503 kb on - strand, within BT4214 at 5551.509 kb on + strand, within BT4214 at 5551.523 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.692 kb on - strand, within BT4214 at 5551.697 kb on + strand, within BT4214 at 5551.712 kb on - strand, within BT4214 at 5551.773 kb on + strand, within BT4214 at 5551.780 kb on - strand, within BT4214 at 5551.780 kb on - strand, within BT4214 at 5551.796 kb on + strand, within BT4214 at 5551.797 kb on - strand, within BT4214 at 5551.861 kb on + strand at 5551.875 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.905 kb on + strand at 5551.975 kb on - strand at 5551.988 kb on + strand at 5551.989 kb on - strand at 5551.991 kb on + strand at 5551.991 kb on + strand at 5551.992 kb on - strand at 5551.992 kb on - strand at 5552.021 kb on + strand at 5552.021 kb on + strand at 5552.022 kb on - strand at 5552.036 kb on - strand at 5552.057 kb on + strand at 5552.089 kb on + strand at 5552.090 kb on - strand at 5552.863 kb on - strand at 5552.863 kb on - strand at 5553.595 kb on - strand at 5553.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 5,551,111 + +0.0 5,551,112 - -2.6 5,551,121 + +0.0 5,551,225 + BT4214 0.18 -0.6 5,551,225 + BT4214 0.18 -2.3 5,551,225 + BT4214 0.18 -0.5 5,551,226 - BT4214 0.18 +0.0 5,551,226 - BT4214 0.18 -1.0 5,551,226 - BT4214 0.18 -2.0 5,551,230 - BT4214 0.19 -1.6 5,551,230 - BT4214 0.19 -2.5 5,551,340 + BT4214 0.31 -0.5 5,551,345 + BT4214 0.32 -1.0 5,551,407 + BT4214 0.39 -1.0 5,551,448 - BT4214 0.44 +0.0 5,551,503 - BT4214 0.50 +0.0 5,551,503 - BT4214 0.50 -1.6 5,551,509 + BT4214 0.51 -0.5 5,551,523 + BT4214 0.52 -2.1 5,551,649 + BT4214 0.67 -2.2 5,551,649 + BT4214 0.67 +0.7 5,551,649 + BT4214 0.67 +0.0 5,551,692 - BT4214 0.71 +0.0 5,551,697 + BT4214 0.72 -1.5 5,551,712 - BT4214 0.74 +0.0 5,551,773 + BT4214 0.81 -1.0 5,551,780 - BT4214 0.82 -2.7 5,551,780 - BT4214 0.82 +2.6 5,551,796 + BT4214 0.83 -1.6 5,551,797 - BT4214 0.84 -2.0 5,551,861 + +1.1 5,551,875 - +0.0 5,551,877 - -1.0 5,551,877 - -0.5 5,551,877 - -1.1 5,551,877 - -1.2 5,551,877 - +2.0 5,551,905 + +0.0 5,551,975 - -2.0 5,551,988 + +0.0 5,551,989 - -2.3 5,551,991 + -2.3 5,551,991 + -2.3 5,551,992 - -2.8 5,551,992 - +0.0 5,552,021 + +0.0 5,552,021 + -1.3 5,552,022 - -2.3 5,552,036 - -1.0 5,552,057 + -1.6 5,552,089 + -0.9 5,552,090 - -3.2 5,552,863 - +1.0 5,552,863 - +0.6 5,553,595 - -1.0 5,553,620 - +1.7
Or see this region's nucleotide sequence