Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3035

Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3033 and BT3034 overlap by 4 nucleotidesBT3034 and BT3035 are separated by 105 nucleotidesBT3035 and BT3036 are separated by 80 nucleotides BT3033: BT3033 - DNA topoisomerase IV subunit B (NCBI ptt file), at 3,840,014 to 3,841,891 BT3033 BT3034: BT3034 - phosphopantetheine adenylyltransferase (NCBI ptt file), at 3,841,888 to 3,842,343 BT3034 BT3035: BT3035 - carboxy-terminal processing protease precursor (NCBI ptt file), at 3,842,449 to 3,844,038 BT3035 BT3036: BT3036 - conserved hypothetical protein (NCBI ptt file), at 3,844,119 to 3,846,701 BT3036 Position (kb) 3842 3843 3844 3845Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3842.376 kb on - strandat 3842.376 kb on - strandat 3842.402 kb on - strandat 3842.472 kb on - strandat 3842.474 kb on - strandat 3842.594 kb on + strandat 3842.874 kb on + strand, within BT3035at 3842.875 kb on - strand, within BT3035at 3842.973 kb on + strand, within BT3035at 3842.973 kb on + strand, within BT3035at 3842.978 kb on + strand, within BT3035at 3842.978 kb on + strand, within BT3035at 3842.978 kb on + strand, within BT3035at 3842.979 kb on - strand, within BT3035at 3843.004 kb on + strand, within BT3035at 3843.148 kb on + strand, within BT3035at 3843.302 kb on - strand, within BT3035at 3843.357 kb on + strand, within BT3035at 3843.365 kb on + strand, within BT3035at 3843.451 kb on + strand, within BT3035at 3843.452 kb on - strand, within BT3035at 3843.455 kb on - strand, within BT3035at 3843.506 kb on - strand, within BT3035at 3843.510 kb on + strand, within BT3035at 3843.521 kb on + strand, within BT3035at 3843.522 kb on - strand, within BT3035at 3843.522 kb on - strand, within BT3035at 3843.660 kb on + strand, within BT3035at 3843.670 kb on + strand, within BT3035at 3843.762 kb on + strand, within BT3035at 3843.766 kb on + strand, within BT3035at 3843.766 kb on + strand, within BT3035at 3843.967 kb on - strandat 3843.967 kb on - strandat 3844.089 kb on + strandat 3844.090 kb on - strandat 3844.090 kb on - strandat 3844.093 kb on - strandat 3844.099 kb on - strandat 3844.118 kb on + strandat 3844.118 kb on + strandat 3844.124 kb on - strandat 3844.124 kb on - strandat 3844.425 kb on + strand, within BT3036at 3844.480 kb on + strand, within BT3036at 3844.481 kb on - strand, within BT3036at 3844.482 kb on + strand, within BT3036at 3844.629 kb on + strand, within BT3036at 3844.630 kb on - strand, within BT3036at 3844.633 kb on + strand, within BT3036at 3844.633 kb on + strand, within BT3036at 3844.634 kb on - strand, within BT3036at 3844.714 kb on + strand, within BT3036at 3844.714 kb on + strand, within BT3036at 3844.715 kb on - strand, within BT3036at 3844.742 kb on - strand, within BT3036at 3844.775 kb on - strand, within BT3036at 3844.892 kb on - strand, within BT3036at 3844.952 kb on - strand, within BT3036at 3844.952 kb on - strand, within BT3036

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
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3,842,376 - -3.1
3,842,376 - -1.1
3,842,402 - -0.5
3,842,472 - -3.6
3,842,474 - -4.5
3,842,594 + +1.9
3,842,874 + BT3035 0.27 -3.6
3,842,875 - BT3035 0.27 -3.1
3,842,973 + BT3035 0.33 -1.6
3,842,973 + BT3035 0.33 -0.1
3,842,978 + BT3035 0.33 -2.9
3,842,978 + BT3035 0.33 -2.4
3,842,978 + BT3035 0.33 -1.6
3,842,979 - BT3035 0.33 -4.0
3,843,004 + BT3035 0.35 -2.4
3,843,148 + BT3035 0.44 -2.1
3,843,302 - BT3035 0.54 -1.1
3,843,357 + BT3035 0.57 -3.1
3,843,365 + BT3035 0.58 -2.6
3,843,451 + BT3035 0.63 -0.1
3,843,452 - BT3035 0.63 -2.9
3,843,455 - BT3035 0.63 -2.1
3,843,506 - BT3035 0.66 -0.1
3,843,510 + BT3035 0.67 -5.1
3,843,521 + BT3035 0.67 -1.1
3,843,522 - BT3035 0.67 -5.1
3,843,522 - BT3035 0.67 -2.8
3,843,660 + BT3035 0.76 -2.1
3,843,670 + BT3035 0.77 -2.9
3,843,762 + BT3035 0.83 -3.7
3,843,766 + BT3035 0.83 -0.1
3,843,766 + BT3035 0.83 -0.1
3,843,967 - -1.1
3,843,967 - -0.1
3,844,089 + -1.2
3,844,090 - -0.8
3,844,090 - -1.1
3,844,093 - -0.1
3,844,099 - -1.1
3,844,118 + -3.1
3,844,118 + -3.3
3,844,124 - -1.6
3,844,124 - -1.1
3,844,425 + BT3036 0.12 -2.4
3,844,480 + BT3036 0.14 -2.8
3,844,481 - BT3036 0.14 -1.8
3,844,482 + BT3036 0.14 -1.1
3,844,629 + BT3036 0.20 -1.1
3,844,630 - BT3036 0.20 -0.1
3,844,633 + BT3036 0.20 -3.0
3,844,633 + BT3036 0.20 -0.1
3,844,634 - BT3036 0.20 -0.1
3,844,714 + BT3036 0.23 -0.1
3,844,714 + BT3036 0.23 -2.0
3,844,715 - BT3036 0.23 -0.1
3,844,742 - BT3036 0.24 +0.3
3,844,775 - BT3036 0.25 -1.8
3,844,892 - BT3036 0.30 -0.1
3,844,952 - BT3036 0.32 -2.1
3,844,952 - BT3036 0.32 +0.9

Or see this region's nucleotide sequence