Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2752 and BT2753 are separated by 201 nucleotides BT2753 and BT2754 are separated by 58 nucleotides
BT2752: BT2752 - primosomal protein N' (replication factor Y) (NCBI ptt file), at 3,410,820 to 3,413,276
BT2752
BT2753: BT2753 - hypothetical protein (NCBI ptt file), at 3,413,478 to 3,414,068
BT2753
BT2754: BT2754 - conserved hypothetical protein (NCBI ptt file), at 3,414,127 to 3,415,107
BT2754
Position (kb)
3413
3414
3415 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3413.345 kb on + strand at 3413.372 kb on + strand at 3413.372 kb on + strand at 3413.468 kb on - strand at 3413.489 kb on - strand at 3413.570 kb on + strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.577 kb on - strand, within BT2753 at 3413.583 kb on + strand, within BT2753 at 3413.584 kb on - strand, within BT2753 at 3413.667 kb on + strand, within BT2753 at 3413.668 kb on - strand, within BT2753 at 3413.675 kb on + strand, within BT2753 at 3413.675 kb on + strand, within BT2753 at 3413.678 kb on + strand, within BT2753 at 3413.719 kb on + strand, within BT2753 at 3413.720 kb on - strand, within BT2753 at 3413.720 kb on - strand at 3413.753 kb on - strand, within BT2753 at 3413.879 kb on + strand, within BT2753 at 3413.963 kb on + strand, within BT2753 at 3413.973 kb on + strand, within BT2753 at 3413.973 kb on + strand, within BT2753 at 3413.985 kb on + strand, within BT2753 at 3414.029 kb on + strand at 3414.029 kb on + strand at 3414.029 kb on + strand at 3414.033 kb on + strand at 3414.034 kb on - strand at 3414.034 kb on - strand at 3414.034 kb on - strand at 3414.068 kb on + strand at 3414.074 kb on + strand at 3414.083 kb on - strand at 3414.101 kb on - strand at 3414.104 kb on + strand at 3414.105 kb on - strand at 3414.108 kb on + strand at 3414.128 kb on + strand at 3414.177 kb on + strand at 3414.177 kb on + strand at 3414.177 kb on + strand at 3414.210 kb on + strand at 3414.217 kb on - strand at 3414.304 kb on + strand, within BT2754 at 3414.316 kb on - strand, within BT2754 at 3414.330 kb on - strand, within BT2754 at 3414.442 kb on + strand, within BT2754 at 3414.579 kb on + strand, within BT2754 at 3414.647 kb on + strand, within BT2754 at 3414.647 kb on + strand, within BT2754 at 3414.650 kb on + strand, within BT2754 at 3414.650 kb on + strand, within BT2754 at 3414.726 kb on + strand, within BT2754 at 3414.727 kb on - strand, within BT2754 at 3414.828 kb on - strand, within BT2754 at 3414.864 kb on + strand, within BT2754 at 3414.865 kb on - strand, within BT2754 at 3414.892 kb on - strand, within BT2754 at 3414.977 kb on + strand, within BT2754 at 3415.009 kb on - strand, within BT2754 at 3415.033 kb on - strand at 3415.034 kb on + strand at 3415.035 kb on - strand at 3415.044 kb on + strand at 3415.044 kb on + strand at 3415.044 kb on + strand at 3415.049 kb on + strand at 3415.049 kb on + strand at 3415.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 3,413,345 + -2.0 3,413,372 + -1.9 3,413,372 + -2.5 3,413,468 - -0.0 3,413,489 - -2.1 3,413,570 + BT2753 0.16 +0.2 3,413,577 - BT2753 0.17 +0.4 3,413,577 - BT2753 0.17 -2.0 3,413,577 - BT2753 0.17 +1.2 3,413,577 - BT2753 0.17 -2.0 3,413,583 + BT2753 0.18 -1.3 3,413,584 - BT2753 0.18 -2.6 3,413,667 + BT2753 0.32 -0.7 3,413,668 - BT2753 0.32 -0.0 3,413,675 + BT2753 0.33 -0.0 3,413,675 + BT2753 0.33 +1.6 3,413,678 + BT2753 0.34 -1.3 3,413,719 + BT2753 0.41 -0.0 3,413,720 - BT2753 0.41 -1.6 3,413,720 - +0.8 3,413,753 - BT2753 0.47 -0.2 3,413,879 + BT2753 0.68 -0.1 3,413,963 + BT2753 0.82 -0.8 3,413,973 + BT2753 0.84 -2.8 3,413,973 + BT2753 0.84 -2.6 3,413,985 + BT2753 0.86 -1.3 3,414,029 + +3.9 3,414,029 + -1.3 3,414,029 + -2.3 3,414,033 + +2.9 3,414,034 - -2.0 3,414,034 - -0.0 3,414,034 - -2.3 3,414,068 + +2.0 3,414,074 + -0.0 3,414,083 - -1.0 3,414,101 - -1.3 3,414,104 + +2.0 3,414,105 - -0.0 3,414,108 + -1.2 3,414,128 + +1.2 3,414,177 + -0.3 3,414,177 + -2.2 3,414,177 + -0.8 3,414,210 + +1.1 3,414,217 - -1.1 3,414,304 + BT2754 0.18 -2.2 3,414,316 - BT2754 0.19 -3.3 3,414,330 - BT2754 0.21 -1.8 3,414,442 + BT2754 0.32 +1.1 3,414,579 + BT2754 0.46 -1.9 3,414,647 + BT2754 0.53 -3.2 3,414,647 + BT2754 0.53 -2.0 3,414,650 + BT2754 0.53 -1.6 3,414,650 + BT2754 0.53 -2.3 3,414,726 + BT2754 0.61 -2.0 3,414,727 - BT2754 0.61 -1.6 3,414,828 - BT2754 0.71 -0.3 3,414,864 + BT2754 0.75 -1.0 3,414,865 - BT2754 0.75 -3.0 3,414,892 - BT2754 0.78 -1.0 3,414,977 + BT2754 0.87 -0.0 3,415,009 - BT2754 0.90 -1.3 3,415,033 - -2.4 3,415,034 + -0.0 3,415,035 - -1.6 3,415,044 + -2.2 3,415,044 + -0.0 3,415,044 + -0.0 3,415,049 + -1.0 3,415,049 + -3.3 3,415,050 - -0.0
Or see this region's nucleotide sequence