Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1672 and BT1673 are separated by 205 nucleotides BT1673 and BT1674 are separated by 11 nucleotides
BT1672: BT1672 - phosphoglycerate kinase (NCBI ptt file), at 2,064,359 to 2,065,618
BT1672
BT1673: BT1673 - thiamine biosynthesis protein, putative (NCBI ptt file), at 2,065,824 to 2,066,678
BT1673
BT1674: BT1674 - hypothetical protein (NCBI ptt file), at 2,066,690 to 2,067,733
BT1674
Position (kb)
2065
2066
2067 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2065.625 kb on + strand at 2065.645 kb on + strand at 2065.679 kb on + strand at 2065.679 kb on + strand at 2065.683 kb on + strand at 2065.683 kb on + strand at 2065.683 kb on + strand at 2065.685 kb on + strand at 2065.686 kb on - strand at 2065.702 kb on - strand at 2065.726 kb on + strand at 2065.727 kb on - strand at 2065.752 kb on + strand at 2065.753 kb on - strand at 2065.788 kb on - strand at 2065.789 kb on + strand at 2065.805 kb on - strand at 2065.862 kb on + strand at 2065.862 kb on + strand at 2065.863 kb on - strand at 2065.999 kb on + strand, within BT1673 at 2066.244 kb on + strand, within BT1673 at 2066.245 kb on - strand, within BT1673 at 2066.436 kb on + strand, within BT1673 at 2066.436 kb on + strand, within BT1673 at 2066.474 kb on - strand, within BT1673 at 2066.474 kb on - strand, within BT1673 at 2066.575 kb on + strand, within BT1673 at 2066.613 kb on + strand at 2066.613 kb on + strand at 2066.640 kb on - strand at 2066.640 kb on - strand at 2066.640 kb on - strand at 2066.666 kb on + strand at 2066.669 kb on + strand at 2066.749 kb on + strand at 2066.787 kb on + strand at 2066.788 kb on - strand at 2066.842 kb on + strand, within BT1674 at 2066.842 kb on + strand, within BT1674 at 2066.946 kb on - strand, within BT1674 at 2066.966 kb on - strand, within BT1674 at 2066.984 kb on - strand, within BT1674 at 2067.020 kb on - strand, within BT1674 at 2067.044 kb on - strand, within BT1674 at 2067.093 kb on - strand, within BT1674 at 2067.172 kb on + strand, within BT1674 at 2067.173 kb on - strand, within BT1674 at 2067.355 kb on - strand, within BT1674 at 2067.431 kb on - strand, within BT1674 at 2067.433 kb on + strand, within BT1674 at 2067.620 kb on + strand, within BT1674
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 2,065,625 + -0.3 2,065,645 + -2.8 2,065,679 + -4.5 2,065,679 + -0.6 2,065,683 + -0.6 2,065,683 + -1.4 2,065,683 + -3.6 2,065,685 + -1.8 2,065,686 - +1.0 2,065,702 - +1.0 2,065,726 + -0.0 2,065,727 - +0.2 2,065,752 + -0.0 2,065,753 - -2.3 2,065,788 - -0.0 2,065,789 + -2.0 2,065,805 - -2.6 2,065,862 + -0.5 2,065,862 + -1.0 2,065,863 - -3.5 2,065,999 + BT1673 0.20 -1.0 2,066,244 + BT1673 0.49 -1.0 2,066,245 - BT1673 0.49 +2.2 2,066,436 + BT1673 0.72 -0.0 2,066,436 + BT1673 0.72 -0.4 2,066,474 - BT1673 0.76 +0.4 2,066,474 - BT1673 0.76 -0.0 2,066,575 + BT1673 0.88 -1.2 2,066,613 + +0.6 2,066,613 + -2.0 2,066,640 - -1.5 2,066,640 - -3.3 2,066,640 - -1.0 2,066,666 + +1.3 2,066,669 + +0.6 2,066,749 + -1.7 2,066,787 + -2.8 2,066,788 - -1.6 2,066,842 + BT1674 0.15 -3.3 2,066,842 + BT1674 0.15 -4.2 2,066,946 - BT1674 0.25 +0.4 2,066,966 - BT1674 0.26 +0.3 2,066,984 - BT1674 0.28 +0.0 2,067,020 - BT1674 0.32 -0.9 2,067,044 - BT1674 0.34 +0.4 2,067,093 - BT1674 0.39 -1.0 2,067,172 + BT1674 0.46 +0.0 2,067,173 - BT1674 0.46 +1.0 2,067,355 - BT1674 0.64 -2.0 2,067,431 - BT1674 0.71 -1.2 2,067,433 + BT1674 0.71 -1.6 2,067,620 + BT1674 0.89 +0.0
Or see this region's nucleotide sequence