Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0463 and BT0464 are separated by 30 nucleotides BT0464 and BT0465 are separated by 3 nucleotides BT0465 and BT0466 are separated by 2 nucleotides
BT0463: BT0463 - glucose-1-phosphate thymidylyltransferase (NCBI ptt file), at 573,896 to 574,783
BT0463
BT0464: BT0464 - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI ptt file), at 574,814 to 575,383
BT0464
BT0465: BT0465 - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 575,387 to 576,241
BT0465
BT0466: BT0466 - dTDP-glucose 4,6-dehydratase (NCBI ptt file), at 576,244 to 577,356
BT0466
Position (kb) 574
575
576 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 573.880 kb on + strand at 573.977 kb on - strand at 573.999 kb on - strand, within BT0463 at 574.031 kb on - strand, within BT0463 at 574.062 kb on - strand, within BT0463 at 574.288 kb on + strand, within BT0463 at 574.352 kb on - strand, within BT0463 at 574.430 kb on + strand, within BT0463 at 574.435 kb on + strand, within BT0463 at 574.462 kb on + strand, within BT0463 at 574.549 kb on - strand, within BT0463 at 574.549 kb on - strand, within BT0463 at 574.664 kb on - strand, within BT0463 at 574.691 kb on - strand, within BT0463 at 574.720 kb on + strand at 574.746 kb on - strand at 574.791 kb on - strand at 574.823 kb on - strand at 574.840 kb on - strand at 574.948 kb on - strand, within BT0464 at 574.964 kb on - strand, within BT0464 at 574.964 kb on - strand, within BT0464 at 574.992 kb on + strand, within BT0464 at 574.992 kb on + strand, within BT0464 at 575.041 kb on + strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.107 kb on - strand, within BT0464 at 575.128 kb on + strand, within BT0464 at 575.376 kb on + strand at 575.410 kb on + strand at 575.461 kb on + strand at 575.470 kb on - strand at 575.527 kb on - strand, within BT0465 at 575.547 kb on + strand, within BT0465 at 575.582 kb on - strand, within BT0465 at 575.624 kb on - strand, within BT0465 at 575.680 kb on + strand, within BT0465 at 575.710 kb on + strand, within BT0465 at 575.716 kb on + strand, within BT0465 at 575.744 kb on - strand, within BT0465 at 575.840 kb on + strand, within BT0465 at 575.894 kb on - strand, within BT0465 at 575.936 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.005 kb on - strand, within BT0465 at 576.008 kb on + strand, within BT0465 at 576.148 kb on - strand, within BT0465 at 576.155 kb on - strand, within BT0465 at 576.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 573,880 + -3.0 573,977 - -1.0 573,999 - BT0463 0.12 -0.0 574,031 - BT0463 0.15 -0.0 574,062 - BT0463 0.19 -2.0 574,288 + BT0463 0.44 -1.0 574,352 - BT0463 0.51 -2.0 574,430 + BT0463 0.60 +1.8 574,435 + BT0463 0.61 -1.9 574,462 + BT0463 0.64 -1.6 574,549 - BT0463 0.74 -1.0 574,549 - BT0463 0.74 +1.0 574,664 - BT0463 0.86 -0.0 574,691 - BT0463 0.90 -1.6 574,720 + -1.1 574,746 - -1.0 574,791 - -1.8 574,823 - -2.0 574,840 - -2.8 574,948 - BT0464 0.24 +0.6 574,964 - BT0464 0.26 -1.0 574,964 - BT0464 0.26 -2.8 574,992 + BT0464 0.31 -0.0 574,992 + BT0464 0.31 +1.3 575,041 + BT0464 0.40 -2.0 575,107 - BT0464 0.51 -1.6 575,107 - BT0464 0.51 -1.6 575,107 - BT0464 0.51 -0.0 575,128 + BT0464 0.55 -1.0 575,376 + -0.0 575,410 + -1.0 575,461 + -2.8 575,470 - +3.2 575,527 - BT0465 0.16 -2.3 575,547 + BT0465 0.19 -0.0 575,582 - BT0465 0.23 -0.6 575,624 - BT0465 0.28 -1.0 575,680 + BT0465 0.34 -3.2 575,710 + BT0465 0.38 -0.0 575,716 + BT0465 0.38 -0.0 575,744 - BT0465 0.42 -1.0 575,840 + BT0465 0.53 -1.6 575,894 - BT0465 0.59 -0.1 575,936 - BT0465 0.64 -1.3 576,005 - BT0465 0.72 -0.0 576,005 - BT0465 0.72 -2.3 576,005 - BT0465 0.72 -1.0 576,008 + BT0465 0.73 -0.0 576,148 - BT0465 0.89 -2.0 576,155 - BT0465 0.90 -0.6 576,340 - -0.4
Or see this region's nucleotide sequence