Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0114 and BT0115 are separated by 12 nucleotides BT0115 and BT0116 are separated by 4 nucleotides
BT0114: BT0114 - putative symporter, arsenic resistance membrane protein (NCBI ptt file), at 105,527 to 106,573
BT0114
BT0115: BT0115 - arsenate reductase (NCBI ptt file), at 106,586 to 107,014
BT0115
BT0116: BT0116 - arsenical pump-driving ATPase (NCBI ptt file), at 107,019 to 108,731
BT0116
Position (kb) 106
107
108 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 105.815 kb on - strand, within BT0114 at 105.818 kb on + strand, within BT0114 at 105.935 kb on + strand, within BT0114 at 105.935 kb on + strand, within BT0114 at 105.968 kb on + strand, within BT0114 at 105.971 kb on + strand, within BT0114 at 106.017 kb on - strand, within BT0114 at 106.018 kb on + strand, within BT0114 at 106.094 kb on + strand, within BT0114 at 106.177 kb on + strand, within BT0114 at 106.216 kb on - strand, within BT0114 at 106.268 kb on + strand, within BT0114 at 106.269 kb on - strand, within BT0114 at 106.269 kb on - strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.272 kb on + strand, within BT0114 at 106.273 kb on - strand, within BT0114 at 106.395 kb on + strand, within BT0114 at 106.427 kb on + strand, within BT0114 at 106.427 kb on + strand, within BT0114 at 106.482 kb on + strand at 106.483 kb on - strand at 106.483 kb on - strand at 106.511 kb on - strand at 106.529 kb on - strand at 106.537 kb on - strand at 106.640 kb on + strand, within BT0115 at 106.640 kb on + strand, within BT0115 at 106.747 kb on + strand, within BT0115 at 106.794 kb on + strand, within BT0115 at 106.815 kb on + strand, within BT0115 at 106.877 kb on + strand, within BT0115 at 106.987 kb on + strand at 107.020 kb on + strand at 107.020 kb on + strand at 107.154 kb on + strand at 107.277 kb on + strand, within BT0116 at 107.332 kb on - strand, within BT0116 at 107.494 kb on - strand, within BT0116 at 107.498 kb on - strand, within BT0116 at 107.582 kb on + strand, within BT0116 at 107.788 kb on - strand, within BT0116 at 107.832 kb on - strand, within BT0116 at 107.847 kb on + strand, within BT0116 at 107.852 kb on + strand, within BT0116 at 107.870 kb on + strand, within BT0116 at 107.904 kb on - strand, within BT0116 at 107.906 kb on - strand, within BT0116 at 107.937 kb on - strand, within BT0116 at 107.966 kb on - strand, within BT0116
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 105,815 - BT0114 0.28 -1.0 105,818 + BT0114 0.28 -1.2 105,935 + BT0114 0.39 -1.2 105,935 + BT0114 0.39 +1.4 105,968 + BT0114 0.42 -1.0 105,971 + BT0114 0.42 -2.0 106,017 - BT0114 0.47 -0.5 106,018 + BT0114 0.47 -0.9 106,094 + BT0114 0.54 -1.8 106,177 + BT0114 0.62 -1.0 106,216 - BT0114 0.66 -1.0 106,268 + BT0114 0.71 -1.0 106,269 - BT0114 0.71 -2.6 106,269 - BT0114 0.71 -3.3 106,272 + BT0114 0.71 +0.0 106,272 + BT0114 0.71 -2.0 106,272 + BT0114 0.71 +0.0 106,273 - BT0114 0.71 -2.0 106,395 + BT0114 0.83 -1.6 106,427 + BT0114 0.86 +0.0 106,427 + BT0114 0.86 -2.3 106,482 + +0.0 106,483 - -0.9 106,483 - -1.0 106,511 - -0.7 106,529 - -2.0 106,537 - -0.7 106,640 + BT0115 0.13 -2.8 106,640 + BT0115 0.13 -2.0 106,747 + BT0115 0.38 -3.3 106,794 + BT0115 0.48 -2.3 106,815 + BT0115 0.53 -3.8 106,877 + BT0115 0.68 -1.0 106,987 + -1.0 107,020 + -0.9 107,020 + -2.0 107,154 + -0.5 107,277 + BT0116 0.15 -2.0 107,332 - BT0116 0.18 +0.7 107,494 - BT0116 0.28 -2.8 107,498 - BT0116 0.28 -1.0 107,582 + BT0116 0.33 -1.6 107,788 - BT0116 0.45 -1.2 107,832 - BT0116 0.47 -2.0 107,847 + BT0116 0.48 +0.6 107,852 + BT0116 0.49 -1.5 107,870 + BT0116 0.50 -0.4 107,904 - BT0116 0.52 +0.0 107,906 - BT0116 0.52 -2.3 107,937 - BT0116 0.54 -1.9 107,966 - BT0116 0.55 +2.8
Or see this region's nucleotide sequence