Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0626 and BT0627 are separated by 35 nucleotides BT0627 and BT0628 are separated by 7 nucleotides BT0628 and BT0629 are separated by 161 nucleotides
BT0626: BT0626 - phenylalanyl-tRNA synthetase beta chain (NCBI ptt file), at 771,763 to 774,225
BT0626
BT0627: BT0627 - conserved hypothetical protein, with a conserved domain of unknown function (NCBI ptt file), at 774,261 to 775,004
BT0627
BT0628: BT0628 - conserved hypothetical protein (NCBI ptt file), at 775,012 to 775,257
BT0628
BT0629: BT0629 - Mn2+ and Fe2+ transport protein (NCBI ptt file), at 775,419 to 776,675
BT0629
Position (kb) 774
775
776 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 774.223 kb on + strand at 775.018 kb on + strand at 775.027 kb on - strand at 775.110 kb on + strand, within BT0628 at 775.110 kb on + strand, within BT0628 at 775.281 kb on + strand at 775.320 kb on + strand at 775.385 kb on - strand at 775.386 kb on + strand at 775.387 kb on - strand at 775.387 kb on - strand at 775.387 kb on - strand at 775.391 kb on - strand at 775.391 kb on - strand at 775.393 kb on - strand at 775.490 kb on + strand at 775.495 kb on - strand at 775.637 kb on + strand, within BT0629 at 775.695 kb on + strand, within BT0629 at 775.814 kb on + strand, within BT0629 at 775.817 kb on + strand, within BT0629 at 775.931 kb on + strand, within BT0629 at 775.958 kb on - strand, within BT0629
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen remove 774,223 + -1.2 775,018 + -0.7 775,027 - -1.0 775,110 + BT0628 0.40 -0.0 775,110 + BT0628 0.40 -1.7 775,281 + -1.5 775,320 + -1.6 775,385 - -0.0 775,386 + -1.9 775,387 - +1.0 775,387 - -0.4 775,387 - -2.6 775,391 - -0.4 775,391 - -1.6 775,393 - -0.0 775,490 + -0.0 775,495 - -3.3 775,637 + BT0629 0.17 -0.0 775,695 + BT0629 0.22 -0.0 775,814 + BT0629 0.31 -0.6 775,817 + BT0629 0.32 -2.0 775,931 + BT0629 0.41 -2.0 775,958 - BT0629 0.43 -2.3
Or see this region's nucleotide sequence