Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3509 and BT3510 are separated by 202 nucleotides BT3510 and BT3511 are separated by 44 nucleotides BT3511 and BT3512 are separated by 97 nucleotides
BT3509: BT3509 - conserved hypothetical protein (NCBI ptt file), at 4,529,146 to 4,529,739
BT3509
BT3510: BT3510 - hypothetical protein (NCBI ptt file), at 4,529,942 to 4,530,271
BT3510
BT3511: BT3511 - hypothetical protein (NCBI ptt file), at 4,530,316 to 4,530,819
BT3511
BT3512: BT3512 - glutaminase A (NCBI ptt file), at 4,530,917 to 4,533,430
BT3512
Position (kb)
4529
4530
4531 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4528.942 kb on - strand at 4528.944 kb on - strand at 4528.947 kb on + strand at 4528.947 kb on + strand at 4529.104 kb on - strand at 4529.104 kb on - strand at 4529.135 kb on + strand at 4529.135 kb on + strand at 4529.171 kb on + strand at 4529.195 kb on + strand at 4529.339 kb on - strand, within BT3509 at 4529.361 kb on - strand, within BT3509 at 4529.435 kb on - strand, within BT3509 at 4529.437 kb on + strand, within BT3509 at 4529.437 kb on + strand, within BT3509 at 4529.490 kb on + strand, within BT3509 at 4529.501 kb on - strand, within BT3509 at 4529.574 kb on - strand, within BT3509 at 4529.658 kb on - strand, within BT3509 at 4529.681 kb on + strand at 4529.711 kb on + strand at 4529.712 kb on - strand at 4529.726 kb on + strand at 4529.727 kb on - strand at 4529.774 kb on + strand at 4529.940 kb on - strand at 4529.941 kb on + strand at 4529.965 kb on - strand at 4530.003 kb on - strand, within BT3510 at 4530.236 kb on - strand, within BT3510 at 4530.287 kb on - strand at 4530.289 kb on + strand at 4530.316 kb on - strand at 4530.332 kb on + strand at 4530.342 kb on - strand at 4530.342 kb on - strand at 4530.402 kb on + strand, within BT3511 at 4530.402 kb on + strand, within BT3511 at 4530.451 kb on - strand, within BT3511 at 4530.451 kb on - strand, within BT3511 at 4530.491 kb on - strand, within BT3511 at 4530.499 kb on + strand, within BT3511 at 4530.500 kb on - strand, within BT3511 at 4530.647 kb on + strand, within BT3511 at 4530.694 kb on - strand, within BT3511 at 4530.708 kb on + strand, within BT3511 at 4530.713 kb on - strand, within BT3511 at 4530.714 kb on + strand, within BT3511 at 4530.787 kb on + strand at 4530.788 kb on - strand at 4530.802 kb on - strand at 4530.802 kb on - strand at 4530.804 kb on - strand at 4530.830 kb on - strand at 4530.830 kb on - strand at 4530.862 kb on + strand at 4530.888 kb on + strand at 4530.888 kb on + strand at 4530.891 kb on - strand at 4530.910 kb on - strand at 4530.957 kb on + strand at 4531.030 kb on + strand at 4531.064 kb on - strand at 4531.065 kb on + strand at 4531.093 kb on + strand at 4531.094 kb on - strand at 4531.094 kb on - strand at 4531.094 kb on - strand at 4531.111 kb on + strand at 4531.112 kb on - strand at 4531.112 kb on - strand at 4531.112 kb on - strand at 4531.112 kb on - strand at 4531.124 kb on + strand at 4531.146 kb on + strand at 4531.176 kb on + strand, within BT3512 at 4531.198 kb on - strand, within BT3512 at 4531.221 kb on - strand, within BT3512 at 4531.221 kb on - strand, within BT3512
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen remove 4,528,942 - -1.6 4,528,944 - -1.0 4,528,947 + -3.1 4,528,947 + -2.0 4,529,104 - -0.7 4,529,104 - -1.2 4,529,135 + -2.0 4,529,135 + -1.7 4,529,171 + -2.8 4,529,195 + -1.6 4,529,339 - BT3509 0.32 -0.3 4,529,361 - BT3509 0.36 -1.4 4,529,435 - BT3509 0.49 -2.0 4,529,437 + BT3509 0.49 -0.4 4,529,437 + BT3509 0.49 -1.8 4,529,490 + BT3509 0.58 -1.6 4,529,501 - BT3509 0.60 -1.7 4,529,574 - BT3509 0.72 -2.1 4,529,658 - BT3509 0.86 +0.0 4,529,681 + -2.6 4,529,711 + -3.0 4,529,712 - +0.0 4,529,726 + -1.0 4,529,727 - +0.0 4,529,774 + -2.3 4,529,940 - -2.8 4,529,941 + +0.7 4,529,965 - -3.0 4,530,003 - BT3510 0.18 -2.6 4,530,236 - BT3510 0.89 -2.5 4,530,287 - -1.4 4,530,289 + -2.0 4,530,316 - -1.0 4,530,332 + +2.0 4,530,342 - +1.0 4,530,342 - +0.0 4,530,402 + BT3511 0.17 -1.2 4,530,402 + BT3511 0.17 -1.8 4,530,451 - BT3511 0.27 -0.3 4,530,451 - BT3511 0.27 -4.0 4,530,491 - BT3511 0.35 -1.0 4,530,499 + BT3511 0.36 -2.0 4,530,500 - BT3511 0.37 -2.0 4,530,647 + BT3511 0.66 -1.1 4,530,694 - BT3511 0.75 +0.0 4,530,708 + BT3511 0.78 -2.3 4,530,713 - BT3511 0.79 -1.8 4,530,714 + BT3511 0.79 -0.6 4,530,787 + +0.0 4,530,788 - -1.0 4,530,802 - -1.2 4,530,802 - +0.0 4,530,804 - +0.0 4,530,830 - -1.0 4,530,830 - +0.0 4,530,862 + -1.6 4,530,888 + -3.0 4,530,888 + -1.3 4,530,891 - -0.6 4,530,910 - +0.0 4,530,957 + -1.8 4,531,030 + -2.8 4,531,064 - -1.8 4,531,065 + +0.3 4,531,093 + -1.0 4,531,094 - -1.0 4,531,094 - -1.0 4,531,094 - -2.3 4,531,111 + -1.0 4,531,112 - -1.6 4,531,112 - -2.0 4,531,112 - -0.9 4,531,112 - -2.1 4,531,124 + -1.0 4,531,146 + -1.0 4,531,176 + BT3512 0.10 -2.3 4,531,198 - BT3512 0.11 +1.0 4,531,221 - BT3512 0.12 -0.3 4,531,221 - BT3512 0.12 -2.0
Or see this region's nucleotide sequence