Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1194 and BT1195 overlap by 23 nucleotides BT1195 and BT1196 are separated by 84 nucleotides BT1196 and BT1197 are separated by 209 nucleotides BT1197 and BT1198 are separated by 242 nucleotides
BT1194: BT1194 - outer membrane protein, OmpA family (NCBI ptt file), at 1,490,462 to 1,491,262
BT1194
BT1195: BT1195 - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI ptt file), at 1,491,240 to 1,491,986
BT1195
BT1196: BT1196 - pyruvate carboxylase subunit B) (NCBI ptt file), at 1,492,071 to 1,493,846
BT1196
BT1197: BT1197 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 1,494,056 to 1,494,559
BT1197
BT1198: BT1198 - LemA protein (NCBI ptt file), at 1,494,802 to 1,495,362
BT1198
Position (kb)
1492
1493
1494 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1491.209 kb on + strand at 1491.210 kb on - strand at 1491.215 kb on + strand at 1491.215 kb on + strand at 1491.216 kb on - strand at 1491.352 kb on - strand, within BT1195 at 1491.352 kb on - strand, within BT1195 at 1491.352 kb on - strand, within BT1195 at 1491.383 kb on - strand, within BT1195 at 1491.404 kb on + strand, within BT1195 at 1491.654 kb on + strand, within BT1195 at 1491.676 kb on + strand, within BT1195 at 1491.896 kb on - strand, within BT1195 at 1492.000 kb on + strand at 1492.060 kb on + strand at 1492.073 kb on - strand at 1492.073 kb on - strand at 1492.100 kb on - strand at 1492.185 kb on - strand at 1492.189 kb on + strand at 1492.189 kb on + strand at 1492.190 kb on - strand at 1492.193 kb on + strand at 1492.206 kb on - strand at 1492.238 kb on - strand at 1492.241 kb on + strand at 1492.241 kb on + strand at 1492.442 kb on + strand, within BT1196 at 1492.443 kb on - strand, within BT1196 at 1492.676 kb on + strand, within BT1196 at 1492.919 kb on + strand, within BT1196 at 1492.932 kb on - strand, within BT1196 at 1492.970 kb on + strand, within BT1196 at 1492.982 kb on + strand, within BT1196 at 1493.061 kb on - strand, within BT1196 at 1493.097 kb on - strand, within BT1196 at 1493.163 kb on - strand, within BT1196 at 1493.163 kb on - strand, within BT1196 at 1493.195 kb on + strand, within BT1196 at 1493.372 kb on + strand, within BT1196 at 1493.374 kb on + strand, within BT1196 at 1493.377 kb on + strand, within BT1196 at 1493.408 kb on + strand, within BT1196 at 1493.408 kb on + strand, within BT1196 at 1493.412 kb on + strand, within BT1196 at 1493.499 kb on - strand, within BT1196 at 1493.534 kb on + strand, within BT1196 at 1493.536 kb on + strand, within BT1196 at 1493.603 kb on + strand, within BT1196 at 1493.679 kb on + strand at 1493.680 kb on - strand at 1493.716 kb on - strand at 1493.808 kb on - strand at 1493.873 kb on - strand at 1493.921 kb on - strand at 1493.938 kb on + strand at 1493.950 kb on + strand at 1493.956 kb on + strand at 1493.970 kb on + strand at 1494.197 kb on - strand, within BT1197 at 1494.298 kb on - strand, within BT1197 at 1494.344 kb on - strand, within BT1197 at 1494.382 kb on - strand, within BT1197 at 1494.496 kb on - strand, within BT1197 at 1494.814 kb on - strand at 1494.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen remove 1,491,209 + -2.0 1,491,210 - -1.3 1,491,215 + -1.6 1,491,215 + -0.0 1,491,216 - -1.6 1,491,352 - BT1195 0.15 -1.2 1,491,352 - BT1195 0.15 -1.6 1,491,352 - BT1195 0.15 -0.6 1,491,383 - BT1195 0.19 -2.3 1,491,404 + BT1195 0.22 -0.0 1,491,654 + BT1195 0.55 -0.0 1,491,676 + BT1195 0.58 -0.0 1,491,896 - BT1195 0.88 -3.0 1,492,000 + -2.0 1,492,060 + -2.0 1,492,073 - +0.4 1,492,073 - -2.3 1,492,100 - -2.0 1,492,185 - -1.1 1,492,189 + -0.0 1,492,189 + -2.6 1,492,190 - -2.6 1,492,193 + -1.6 1,492,206 - +1.0 1,492,238 - -1.6 1,492,241 + -2.6 1,492,241 + -2.6 1,492,442 + BT1196 0.21 -2.0 1,492,443 - BT1196 0.21 -1.3 1,492,676 + BT1196 0.34 -0.0 1,492,919 + BT1196 0.48 -2.0 1,492,932 - BT1196 0.48 -1.0 1,492,970 + BT1196 0.51 -2.6 1,492,982 + BT1196 0.51 -2.3 1,493,061 - BT1196 0.56 -1.3 1,493,097 - BT1196 0.58 -3.2 1,493,163 - BT1196 0.61 +0.6 1,493,163 - BT1196 0.61 -0.0 1,493,195 + BT1196 0.63 -2.3 1,493,372 + BT1196 0.73 -0.0 1,493,374 + BT1196 0.73 +1.0 1,493,377 + BT1196 0.74 -2.6 1,493,408 + BT1196 0.75 -1.0 1,493,408 + BT1196 0.75 -0.0 1,493,412 + BT1196 0.76 -3.9 1,493,499 - BT1196 0.80 -1.6 1,493,534 + BT1196 0.82 -1.3 1,493,536 + BT1196 0.82 -1.6 1,493,603 + BT1196 0.86 -0.0 1,493,679 + -2.6 1,493,680 - -1.6 1,493,716 - +1.6 1,493,808 - -2.6 1,493,873 - -1.0 1,493,921 - -2.8 1,493,938 + -1.6 1,493,950 + -3.2 1,493,956 + -2.6 1,493,970 + +2.3 1,494,197 - BT1197 0.28 +2.0 1,494,298 - BT1197 0.48 +0.4 1,494,344 - BT1197 0.57 +2.6 1,494,382 - BT1197 0.65 +1.0 1,494,496 - BT1197 0.87 +1.0 1,494,814 - -0.0 1,494,828 - -0.0
Or see this region's nucleotide sequence