Experiment: L-Serine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1980 and BT1981 are separated by 111 nucleotides BT1981 and BT1982 overlap by 4 nucleotides BT1982 and BT1983 are separated by 2 nucleotides BT1983 and BT1984 are separated by 33 nucleotides BT1984 and BT1985 overlap by 11 nucleotides
BT1980: BT1980 - transposase (NCBI ptt file), at 2,490,471 to 2,492,132
BT1980
BT1981: BT1981 - hypothetical protein (NCBI ptt file), at 2,492,244 to 2,492,549
BT1981
BT1982: BT1982 - hypothetical protein (NCBI ptt file), at 2,492,546 to 2,492,875
BT1982
BT1983: BT1983 - hemolysin III (NCBI ptt file), at 2,492,878 to 2,493,567
BT1983
BT1984: BT1984 - transcriptional regulator (NCBI ptt file), at 2,493,601 to 2,493,822
BT1984
BT1985: BT1985 - hypothetical protein (NCBI ptt file), at 2,493,812 to 2,494,270
BT1985
Position (kb)
2492
2493 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2491.603 kb on - strand, within BT1980 at 2491.634 kb on + strand, within BT1980 at 2491.655 kb on + strand, within BT1980 at 2491.656 kb on - strand, within BT1980 at 2491.712 kb on + strand, within BT1980 at 2491.712 kb on + strand, within BT1980 at 2491.775 kb on + strand, within BT1980 at 2491.775 kb on + strand, within BT1980 at 2491.776 kb on - strand, within BT1980 at 2491.781 kb on - strand, within BT1980 at 2491.985 kb on + strand at 2492.014 kb on + strand at 2492.045 kb on + strand at 2492.051 kb on + strand at 2492.057 kb on + strand at 2492.107 kb on + strand at 2492.108 kb on + strand at 2492.153 kb on - strand at 2492.159 kb on + strand at 2492.427 kb on + strand, within BT1981 at 2492.427 kb on + strand, within BT1981 at 2492.428 kb on - strand, within BT1981 at 2492.431 kb on - strand, within BT1981 at 2492.431 kb on - strand, within BT1981 at 2492.482 kb on + strand, within BT1981 at 2492.537 kb on - strand at 2492.553 kb on + strand at 2492.604 kb on + strand, within BT1982 at 2492.607 kb on + strand, within BT1982 at 2492.724 kb on + strand, within BT1982 at 2492.726 kb on + strand, within BT1982 at 2492.727 kb on - strand, within BT1982 at 2492.937 kb on + strand at 2492.937 kb on + strand at 2492.938 kb on - strand at 2492.979 kb on + strand, within BT1983 at 2492.982 kb on - strand, within BT1983 at 2493.062 kb on + strand, within BT1983 at 2493.094 kb on + strand, within BT1983 at 2493.113 kb on - strand, within BT1983 at 2493.171 kb on + strand, within BT1983 at 2493.176 kb on - strand, within BT1983 at 2493.176 kb on - strand, within BT1983 at 2493.196 kb on + strand, within BT1983 at 2493.340 kb on + strand, within BT1983 at 2493.343 kb on - strand, within BT1983 at 2493.344 kb on + strand, within BT1983 at 2493.348 kb on + strand, within BT1983 at 2493.349 kb on - strand, within BT1983 at 2493.349 kb on - strand, within BT1983 at 2493.451 kb on + strand, within BT1983 at 2493.454 kb on - strand, within BT1983 at 2493.473 kb on - strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.490 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.492 kb on + strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand, within BT1983 at 2493.493 kb on - strand at 2493.534 kb on - strand at 2493.541 kb on - strand at 2493.560 kb on + strand at 2493.560 kb on + strand at 2493.657 kb on - strand, within BT1984 at 2493.665 kb on - strand, within BT1984 at 2493.684 kb on + strand, within BT1984 at 2493.694 kb on - strand, within BT1984 at 2493.794 kb on - strand, within BT1984 at 2493.826 kb on + strand at 2493.827 kb on - strand at 2493.827 kb on - strand at 2493.837 kb on + strand at 2493.855 kb on - strand at 2493.875 kb on + strand, within BT1985
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); Varel_Bryant_medium_Glucose_DTT_noNitrogen remove 2,491,603 - BT1980 0.68 +1.3 2,491,634 + BT1980 0.70 -2.3 2,491,655 + BT1980 0.71 -0.6 2,491,656 - BT1980 0.71 -1.6 2,491,712 + BT1980 0.75 -0.6 2,491,712 + BT1980 0.75 -1.0 2,491,775 + BT1980 0.78 -1.0 2,491,775 + BT1980 0.78 -0.0 2,491,776 - BT1980 0.79 +0.5 2,491,781 - BT1980 0.79 -2.1 2,491,985 + -0.0 2,492,014 + +2.6 2,492,045 + -4.0 2,492,051 + -3.2 2,492,057 + -0.4 2,492,107 + -0.9 2,492,108 + -0.0 2,492,153 - -1.6 2,492,159 + -1.2 2,492,427 + BT1981 0.60 +0.3 2,492,427 + BT1981 0.60 +1.6 2,492,428 - BT1981 0.60 -0.0 2,492,431 - BT1981 0.61 -3.3 2,492,431 - BT1981 0.61 -2.0 2,492,482 + BT1981 0.78 +1.4 2,492,537 - +0.6 2,492,553 + -1.0 2,492,604 + BT1982 0.18 -2.3 2,492,607 + BT1982 0.18 -2.1 2,492,724 + BT1982 0.54 -1.0 2,492,726 + BT1982 0.55 -0.4 2,492,727 - BT1982 0.55 -1.0 2,492,937 + -1.2 2,492,937 + -0.3 2,492,938 - -2.3 2,492,979 + BT1983 0.15 -3.5 2,492,982 - BT1983 0.15 -3.3 2,493,062 + BT1983 0.27 -3.0 2,493,094 + BT1983 0.31 -2.3 2,493,113 - BT1983 0.34 -2.6 2,493,171 + BT1983 0.42 -2.0 2,493,176 - BT1983 0.43 -2.8 2,493,176 - BT1983 0.43 -2.4 2,493,196 + BT1983 0.46 -0.0 2,493,340 + BT1983 0.67 -2.2 2,493,343 - BT1983 0.67 +0.6 2,493,344 + BT1983 0.68 -1.0 2,493,348 + BT1983 0.68 -0.0 2,493,349 - BT1983 0.68 -0.0 2,493,349 - BT1983 0.68 -1.8 2,493,451 + BT1983 0.83 -0.8 2,493,454 - BT1983 0.83 +1.0 2,493,473 - BT1983 0.86 -2.3 2,493,490 + BT1983 0.89 +0.6 2,493,490 + BT1983 0.89 -3.2 2,493,492 + BT1983 0.89 -1.3 2,493,492 + BT1983 0.89 -1.0 2,493,492 + BT1983 0.89 -2.3 2,493,493 - BT1983 0.89 -0.6 2,493,493 - BT1983 0.89 -3.0 2,493,493 - BT1983 0.89 -3.5 2,493,493 - BT1983 0.89 -0.0 2,493,493 - -2.0 2,493,534 - -2.5 2,493,541 - -0.0 2,493,560 + -0.0 2,493,560 + -2.0 2,493,657 - BT1984 0.25 -0.0 2,493,665 - BT1984 0.29 -1.0 2,493,684 + BT1984 0.37 +0.6 2,493,694 - BT1984 0.42 -0.0 2,493,794 - BT1984 0.87 -1.7 2,493,826 + -1.4 2,493,827 - -1.2 2,493,827 - -0.0 2,493,837 + -0.0 2,493,855 - -0.0 2,493,875 + BT1985 0.14 +0.5
Or see this region's nucleotide sequence