Experiment: Rhamnogalacturonan - from potato; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3411 and BT3412 are separated by 465 nucleotides BT3412 and BT3413 are separated by 172 nucleotides
BT3411: BT3411 - pyrophosphate-energized vacuolar membrane proton pump (NCBI ptt file), at 4,393,181 to 4,395,385
BT3411
BT3412: BT3412 - ribonuclease HII (NCBI ptt file), at 4,395,851 to 4,396,453
BT3412
BT3413: BT3413 - hypothetical protein (NCBI ptt file), at 4,396,626 to 4,398,083
BT3413
Position (kb)
4395
4396
4397 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4394.931 kb on + strand, within BT3411 at 4394.997 kb on - strand, within BT3411 at 4395.065 kb on - strand, within BT3411 at 4395.112 kb on - strand, within BT3411 at 4395.157 kb on + strand, within BT3411 at 4395.203 kb on + strand at 4395.207 kb on + strand at 4395.247 kb on + strand at 4395.258 kb on + strand at 4395.262 kb on - strand at 4395.292 kb on + strand at 4395.293 kb on - strand at 4395.293 kb on - strand at 4395.328 kb on + strand at 4395.328 kb on + strand at 4395.329 kb on - strand at 4395.329 kb on - strand at 4395.339 kb on + strand at 4395.340 kb on - strand at 4395.361 kb on + strand at 4395.361 kb on + strand at 4395.403 kb on + strand at 4395.456 kb on + strand at 4395.457 kb on - strand at 4395.457 kb on - strand at 4395.457 kb on - strand at 4395.457 kb on - strand at 4395.538 kb on - strand at 4395.566 kb on + strand at 4395.573 kb on + strand at 4395.594 kb on - strand at 4395.595 kb on + strand at 4395.598 kb on + strand at 4395.599 kb on - strand at 4395.600 kb on + strand at 4395.600 kb on + strand at 4395.635 kb on - strand at 4395.635 kb on - strand at 4395.661 kb on - strand at 4395.733 kb on + strand at 4395.734 kb on - strand at 4395.736 kb on - strand at 4395.846 kb on + strand at 4395.850 kb on + strand at 4395.850 kb on + strand at 4395.850 kb on + strand at 4395.851 kb on - strand at 4395.851 kb on - strand at 4395.851 kb on - strand at 4395.851 kb on - strand at 4395.859 kb on - strand at 4395.863 kb on + strand at 4395.868 kb on - strand at 4396.189 kb on + strand, within BT3412 at 4396.210 kb on + strand, within BT3412 at 4396.213 kb on + strand, within BT3412 at 4396.213 kb on + strand, within BT3412 at 4396.261 kb on + strand, within BT3412 at 4396.261 kb on + strand, within BT3412 at 4396.262 kb on - strand, within BT3412 at 4396.278 kb on - strand, within BT3412 at 4396.278 kb on - strand, within BT3412 at 4396.292 kb on - strand, within BT3412 at 4396.334 kb on - strand, within BT3412 at 4396.454 kb on + strand at 4396.582 kb on + strand at 4396.583 kb on - strand at 4396.583 kb on - strand at 4396.584 kb on + strand at 4396.645 kb on - strand at 4396.672 kb on + strand at 4396.733 kb on + strand at 4396.742 kb on + strand at 4396.743 kb on - strand at 4396.755 kb on - strand at 4396.755 kb on - strand at 4396.800 kb on - strand, within BT3413 at 4396.859 kb on + strand, within BT3413 at 4396.860 kb on - strand, within BT3413 at 4396.862 kb on + strand, within BT3413 at 4396.864 kb on + strand, within BT3413 at 4396.865 kb on - strand, within BT3413 at 4396.865 kb on - strand, within BT3413 at 4396.868 kb on + strand, within BT3413 at 4396.868 kb on + strand, within BT3413 at 4396.869 kb on - strand, within BT3413 at 4396.895 kb on + strand, within BT3413 at 4396.895 kb on + strand, within BT3413 at 4396.895 kb on + strand, within BT3413 at 4396.898 kb on + strand, within BT3413 at 4396.962 kb on - strand, within BT3413 at 4397.011 kb on + strand, within BT3413 at 4397.029 kb on + strand, within BT3413 at 4397.030 kb on - strand, within BT3413 at 4397.041 kb on + strand, within BT3413 at 4397.041 kb on + strand, within BT3413 at 4397.045 kb on + strand, within BT3413 at 4397.046 kb on - strand, within BT3413 at 4397.046 kb on - strand, within BT3413 at 4397.137 kb on + strand, within BT3413 at 4397.145 kb on + strand, within BT3413 at 4397.145 kb on + strand, within BT3413 at 4397.197 kb on + strand at 4397.218 kb on + strand, within BT3413 at 4397.232 kb on - strand, within BT3413 at 4397.248 kb on + strand, within BT3413 at 4397.263 kb on + strand, within BT3413 at 4397.263 kb on + strand, within BT3413 at 4397.264 kb on - strand, within BT3413 at 4397.264 kb on - strand, within BT3413 at 4397.264 kb on - strand, within BT3413 at 4397.322 kb on + strand, within BT3413 at 4397.330 kb on + strand, within BT3413 at 4397.391 kb on - strand, within BT3413 at 4397.418 kb on + strand, within BT3413 at 4397.419 kb on - strand, within BT3413 at 4397.438 kb on + strand, within BT3413
Per-strain Table
Position Strand Gene LocusTag Fraction Rhamnogalacturonan - from potato; autoclaved 2.5 mg/ml (C) remove 4,394,931 + BT3411 0.79 -1.1 4,394,997 - BT3411 0.82 +0.9 4,395,065 - BT3411 0.85 -2.1 4,395,112 - BT3411 0.88 -1.9 4,395,157 + BT3411 0.90 -0.1 4,395,203 + +1.5 4,395,207 + +0.3 4,395,247 + -2.7 4,395,258 + -1.1 4,395,262 - -2.3 4,395,292 + -0.1 4,395,293 - +0.7 4,395,293 - -1.4 4,395,328 + -0.1 4,395,328 + -1.7 4,395,329 - -1.3 4,395,329 - -3.4 4,395,339 + -1.8 4,395,340 - -1.3 4,395,361 + -1.7 4,395,361 + -1.7 4,395,403 + -0.9 4,395,456 + -2.4 4,395,457 - -0.1 4,395,457 - -1.3 4,395,457 - +0.5 4,395,457 - -1.5 4,395,538 - -2.4 4,395,566 + -0.6 4,395,573 + -1.1 4,395,594 - -0.1 4,395,595 + -1.1 4,395,598 + -1.5 4,395,599 - +0.1 4,395,600 + -0.9 4,395,600 + -0.1 4,395,635 - -1.7 4,395,635 - -0.4 4,395,661 - -1.7 4,395,733 + -0.2 4,395,734 - -1.5 4,395,736 - -1.7 4,395,846 + -1.7 4,395,850 + +0.2 4,395,850 + -0.1 4,395,850 + -0.8 4,395,851 - -1.1 4,395,851 - +0.3 4,395,851 - -1.3 4,395,851 - -1.8 4,395,859 - +0.9 4,395,863 + -2.7 4,395,868 - -0.5 4,396,189 + BT3412 0.56 -1.1 4,396,210 + BT3412 0.60 +0.6 4,396,213 + BT3412 0.60 -0.9 4,396,213 + BT3412 0.60 -0.2 4,396,261 + BT3412 0.68 -0.1 4,396,261 + BT3412 0.68 -0.1 4,396,262 - BT3412 0.68 -0.1 4,396,278 - BT3412 0.71 -0.7 4,396,278 - BT3412 0.71 +0.7 4,396,292 - BT3412 0.73 +0.9 4,396,334 - BT3412 0.80 -1.1 4,396,454 + +0.9 4,396,582 + +0.9 4,396,583 - -0.7 4,396,583 - -0.8 4,396,584 + -1.9 4,396,645 - -0.6 4,396,672 + -0.5 4,396,733 + -0.4 4,396,742 + -0.1 4,396,743 - -0.9 4,396,755 - -1.5 4,396,755 - +0.5 4,396,800 - BT3413 0.12 -1.5 4,396,859 + BT3413 0.16 -1.1 4,396,860 - BT3413 0.16 -0.4 4,396,862 + BT3413 0.16 -0.6 4,396,864 + BT3413 0.16 -0.1 4,396,865 - BT3413 0.16 -0.5 4,396,865 - BT3413 0.16 +1.5 4,396,868 + BT3413 0.17 -1.7 4,396,868 + BT3413 0.17 -1.4 4,396,869 - BT3413 0.17 +0.4 4,396,895 + BT3413 0.18 -1.6 4,396,895 + BT3413 0.18 -0.2 4,396,895 + BT3413 0.18 -1.2 4,396,898 + BT3413 0.19 +0.0 4,396,962 - BT3413 0.23 -1.1 4,397,011 + BT3413 0.26 -1.1 4,397,029 + BT3413 0.28 -2.1 4,397,030 - BT3413 0.28 +0.5 4,397,041 + BT3413 0.28 -0.9 4,397,041 + BT3413 0.28 +0.5 4,397,045 + BT3413 0.29 -0.1 4,397,046 - BT3413 0.29 -0.4 4,397,046 - BT3413 0.29 -0.1 4,397,137 + BT3413 0.35 -1.7 4,397,145 + BT3413 0.36 -1.6 4,397,145 + BT3413 0.36 -0.1 4,397,197 + -0.7 4,397,218 + BT3413 0.41 -0.5 4,397,232 - BT3413 0.42 -1.7 4,397,248 + BT3413 0.43 -0.1 4,397,263 + BT3413 0.44 -0.9 4,397,263 + BT3413 0.44 -0.1 4,397,264 - BT3413 0.44 -2.1 4,397,264 - BT3413 0.44 -2.7 4,397,264 - BT3413 0.44 +0.1 4,397,322 + BT3413 0.48 -1.0 4,397,330 + BT3413 0.48 -0.7 4,397,391 - BT3413 0.52 +1.4 4,397,418 + BT3413 0.54 -1.4 4,397,419 - BT3413 0.54 -0.6 4,397,438 + BT3413 0.56 -0.3
Or see this region's nucleotide sequence