Experiment: Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1308 and BT1309 are separated by 81 nucleotides BT1309 and BT1310 are separated by 76 nucleotides BT1310 and BT1311 are separated by 133 nucleotides
BT1308: BT1308 - clostripain-related protein (NCBI ptt file), at 1,632,401 to 1,633,600
BT1308
BT1309: BT1309 - hypothetical protein (NCBI ptt file), at 1,633,682 to 1,634,083
BT1309
BT1310: BT1310 - conserved hypothetical protein (NCBI ptt file), at 1,634,160 to 1,634,816
BT1310
BT1311: BT1311 - RNA polymerase sigma factor rpoD (Sigma-A) (NCBI ptt file), at 1,634,950 to 1,635,810
BT1311
Position (kb)
1633
1634
1635 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1632.727 kb on + strand, within BT1308 at 1632.782 kb on + strand, within BT1308 at 1632.922 kb on + strand, within BT1308 at 1633.049 kb on - strand, within BT1308 at 1633.094 kb on + strand, within BT1308 at 1633.094 kb on + strand, within BT1308 at 1633.221 kb on + strand, within BT1308 at 1633.423 kb on + strand, within BT1308 at 1633.424 kb on - strand, within BT1308 at 1633.488 kb on + strand at 1633.502 kb on - strand at 1633.517 kb on - strand at 1633.523 kb on - strand at 1633.586 kb on - strand at 1633.600 kb on + strand at 1633.600 kb on + strand at 1633.601 kb on - strand at 1633.661 kb on - strand at 1633.661 kb on - strand at 1633.712 kb on - strand at 1633.760 kb on - strand, within BT1309 at 1633.812 kb on + strand, within BT1309 at 1633.820 kb on - strand, within BT1309 at 1633.826 kb on - strand, within BT1309 at 1633.826 kb on - strand, within BT1309 at 1633.826 kb on - strand, within BT1309 at 1633.828 kb on + strand, within BT1309 at 1633.857 kb on + strand, within BT1309 at 1633.879 kb on - strand, within BT1309 at 1633.967 kb on - strand, within BT1309 at 1634.076 kb on + strand at 1634.135 kb on + strand at 1634.162 kb on - strand at 1634.178 kb on + strand at 1634.275 kb on + strand, within BT1310 at 1634.276 kb on - strand, within BT1310 at 1634.287 kb on - strand, within BT1310 at 1634.287 kb on - strand, within BT1310 at 1634.311 kb on + strand, within BT1310 at 1634.311 kb on + strand, within BT1310 at 1634.328 kb on - strand, within BT1310 at 1634.328 kb on - strand, within BT1310 at 1634.330 kb on - strand, within BT1310 at 1634.330 kb on - strand, within BT1310 at 1634.349 kb on - strand, within BT1310 at 1634.402 kb on + strand, within BT1310 at 1634.405 kb on - strand, within BT1310 at 1634.424 kb on - strand, within BT1310 at 1634.425 kb on + strand, within BT1310 at 1634.447 kb on - strand, within BT1310 at 1634.462 kb on + strand, within BT1310 at 1634.463 kb on - strand, within BT1310 at 1634.466 kb on - strand, within BT1310 at 1634.467 kb on + strand, within BT1310 at 1634.612 kb on + strand, within BT1310 at 1634.636 kb on - strand, within BT1310 at 1634.636 kb on - strand, within BT1310 at 1634.826 kb on + strand at 1634.830 kb on - strand at 1634.836 kb on + strand at 1634.864 kb on - strand at 1634.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C) remove 1,632,727 + BT1308 0.27 -0.0 1,632,782 + BT1308 0.32 -0.0 1,632,922 + BT1308 0.43 -0.0 1,633,049 - BT1308 0.54 -2.6 1,633,094 + BT1308 0.58 -1.0 1,633,094 + BT1308 0.58 -0.0 1,633,221 + BT1308 0.68 -2.3 1,633,423 + BT1308 0.85 -0.6 1,633,424 - BT1308 0.85 -2.0 1,633,488 + -1.3 1,633,502 - -2.6 1,633,517 - -1.6 1,633,523 - -0.7 1,633,586 - -2.0 1,633,600 + -1.6 1,633,600 + +1.6 1,633,601 - -1.6 1,633,661 - -0.8 1,633,661 - -0.0 1,633,712 - -2.6 1,633,760 - BT1309 0.19 -2.8 1,633,812 + BT1309 0.32 -1.0 1,633,820 - BT1309 0.34 +1.0 1,633,826 - BT1309 0.36 -2.3 1,633,826 - BT1309 0.36 -1.6 1,633,826 - BT1309 0.36 -2.0 1,633,828 + BT1309 0.36 -3.0 1,633,857 + BT1309 0.44 -0.8 1,633,879 - BT1309 0.49 -1.6 1,633,967 - BT1309 0.71 -1.0 1,634,076 + -1.8 1,634,135 + -1.0 1,634,162 - -0.0 1,634,178 + -0.3 1,634,275 + BT1310 0.18 -0.6 1,634,276 - BT1310 0.18 -1.6 1,634,287 - BT1310 0.19 -0.4 1,634,287 - BT1310 0.19 -1.3 1,634,311 + BT1310 0.23 -0.1 1,634,311 + BT1310 0.23 -0.0 1,634,328 - BT1310 0.26 -0.5 1,634,328 - BT1310 0.26 -0.0 1,634,330 - BT1310 0.26 -2.3 1,634,330 - BT1310 0.26 -1.4 1,634,349 - BT1310 0.29 -0.4 1,634,402 + BT1310 0.37 -2.0 1,634,405 - BT1310 0.37 -1.0 1,634,424 - BT1310 0.40 -1.3 1,634,425 + BT1310 0.40 -1.0 1,634,447 - BT1310 0.44 -3.6 1,634,462 + BT1310 0.46 -2.0 1,634,463 - BT1310 0.46 -0.6 1,634,466 - BT1310 0.47 -1.6 1,634,467 + BT1310 0.47 -0.0 1,634,612 + BT1310 0.69 -1.8 1,634,636 - BT1310 0.72 -2.6 1,634,636 - BT1310 0.72 -2.3 1,634,826 + -1.0 1,634,830 - -1.0 1,634,836 + -1.0 1,634,864 - -0.3 1,634,904 - -2.0
Or see this region's nucleotide sequence