Experiment: Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4251 and BT4252 are separated by 39 nucleotides BT4252 and tRNA-Gly are separated by 61 nucleotides tRNA-Gly and tRNA-Tyr are separated by 5 nucleotides tRNA-Tyr and ribH are separated by 50 nucleotides ribH and BT4254 are separated by 167 nucleotides
BT4251: BT4251 - hypothetical protein (NCBI ptt file), at 5,601,336 to 5,603,183
BT4251
BT4252: BT4252 - putative oxidoreductase (NCBI ptt file), at 5,603,223 to 5,604,482
BT4252
BT_t66: tRNA-Gly - tRNA-Gly (RefSeq), at 5,604,544 to 5,604,616
tRNA-Gly
BT_t67: tRNA-Tyr - tRNA-Tyr (RefSeq), at 5,604,622 to 5,604,707
tRNA-Tyr
BT_4253: ribH - riboflavin synthase subunit beta (RefSeq), at 5,604,758 to 5,605,252
ribH
BT4254: BT4254 - conserved hypothetical protein (NCBI ptt file), at 5,605,420 to 5,606,103
BT4254
Position (kb)
5603
5604
5605 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5602.302 kb on - strand, within BT4251 at 5602.310 kb on + strand, within BT4251 at 5602.314 kb on + strand, within BT4251 at 5602.314 kb on + strand, within BT4251 at 5602.315 kb on - strand, within BT4251 at 5602.315 kb on - strand, within BT4251 at 5602.332 kb on - strand, within BT4251 at 5602.341 kb on - strand, within BT4251 at 5602.342 kb on + strand, within BT4251 at 5602.343 kb on - strand, within BT4251 at 5602.343 kb on - strand, within BT4251 at 5602.383 kb on + strand, within BT4251 at 5602.401 kb on - strand, within BT4251 at 5602.407 kb on - strand, within BT4251 at 5602.453 kb on - strand, within BT4251 at 5602.487 kb on + strand, within BT4251 at 5602.577 kb on + strand, within BT4251 at 5602.680 kb on - strand, within BT4251 at 5602.681 kb on + strand, within BT4251 at 5602.681 kb on + strand, within BT4251 at 5602.789 kb on - strand, within BT4251 at 5602.793 kb on + strand, within BT4251 at 5602.841 kb on + strand, within BT4251 at 5602.844 kb on + strand, within BT4251 at 5602.924 kb on + strand, within BT4251 at 5602.925 kb on - strand, within BT4251 at 5602.925 kb on - strand, within BT4251 at 5603.136 kb on + strand at 5603.137 kb on - strand at 5603.149 kb on - strand at 5603.170 kb on - strand at 5603.199 kb on + strand at 5603.200 kb on + strand at 5603.201 kb on - strand at 5603.213 kb on + strand at 5603.280 kb on - strand at 5603.318 kb on + strand at 5603.400 kb on + strand, within BT4252 at 5603.404 kb on + strand, within BT4252 at 5603.405 kb on - strand, within BT4252 at 5603.471 kb on + strand, within BT4252 at 5603.481 kb on + strand, within BT4252 at 5603.481 kb on + strand, within BT4252 at 5603.485 kb on - strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.576 kb on + strand, within BT4252 at 5603.624 kb on + strand, within BT4252 at 5603.690 kb on + strand, within BT4252 at 5603.690 kb on + strand, within BT4252 at 5603.691 kb on - strand, within BT4252 at 5603.783 kb on - strand, within BT4252 at 5603.861 kb on + strand, within BT4252 at 5603.862 kb on - strand, within BT4252 at 5603.926 kb on + strand, within BT4252 at 5603.926 kb on + strand, within BT4252 at 5603.949 kb on - strand, within BT4252 at 5603.950 kb on + strand, within BT4252 at 5603.950 kb on + strand, within BT4252 at 5603.970 kb on + strand, within BT4252 at 5604.009 kb on - strand, within BT4252 at 5604.029 kb on - strand, within BT4252 at 5604.050 kb on + strand, within BT4252 at 5604.050 kb on + strand, within BT4252 at 5604.129 kb on - strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.157 kb on + strand, within BT4252 at 5604.157 kb on + strand, within BT4252 at 5604.192 kb on - strand, within BT4252 at 5604.256 kb on + strand, within BT4252 at 5604.257 kb on - strand, within BT4252 at 5604.257 kb on - strand, within BT4252 at 5604.384 kb on + strand at 5604.385 kb on - strand at 5604.385 kb on - strand at 5604.406 kb on - strand at 5604.453 kb on + strand at 5604.496 kb on + strand at 5604.496 kb on + strand at 5604.496 kb on + strand at 5605.342 kb on - strand at 5605.342 kb on - strand at 5605.358 kb on - strand at 5605.400 kb on - strand at 5605.405 kb on - strand at 5605.407 kb on - strand at 5605.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C) remove 5,602,302 - BT4251 0.52 +2.6 5,602,310 + BT4251 0.53 -0.6 5,602,314 + BT4251 0.53 -0.0 5,602,314 + BT4251 0.53 -1.8 5,602,315 - BT4251 0.53 -0.3 5,602,315 - BT4251 0.53 -3.0 5,602,332 - BT4251 0.54 +1.0 5,602,341 - BT4251 0.54 +0.7 5,602,342 + BT4251 0.54 -1.3 5,602,343 - BT4251 0.54 -0.0 5,602,343 - BT4251 0.54 -1.0 5,602,383 + BT4251 0.57 -1.6 5,602,401 - BT4251 0.58 -0.0 5,602,407 - BT4251 0.58 -2.0 5,602,453 - BT4251 0.60 -1.6 5,602,487 + BT4251 0.62 -3.2 5,602,577 + BT4251 0.67 -0.0 5,602,680 - BT4251 0.73 +2.3 5,602,681 + BT4251 0.73 -2.3 5,602,681 + BT4251 0.73 -2.0 5,602,789 - BT4251 0.79 -0.0 5,602,793 + BT4251 0.79 -1.0 5,602,841 + BT4251 0.81 -1.6 5,602,844 + BT4251 0.82 +1.0 5,602,924 + BT4251 0.86 -1.6 5,602,925 - BT4251 0.86 +1.0 5,602,925 - BT4251 0.86 -1.8 5,603,136 + -0.0 5,603,137 - -1.0 5,603,149 - -1.0 5,603,170 - +1.0 5,603,199 + -2.3 5,603,200 + -2.1 5,603,201 - -1.8 5,603,213 + -1.0 5,603,280 - +0.2 5,603,318 + -2.4 5,603,400 + BT4252 0.14 +0.6 5,603,404 + BT4252 0.14 -4.5 5,603,405 - BT4252 0.14 -1.4 5,603,471 + BT4252 0.20 -0.2 5,603,481 + BT4252 0.20 -4.9 5,603,481 + BT4252 0.20 +0.7 5,603,485 - BT4252 0.21 -1.0 5,603,516 + BT4252 0.23 -2.3 5,603,516 + BT4252 0.23 -0.0 5,603,516 + BT4252 0.23 -1.0 5,603,576 + BT4252 0.28 -1.0 5,603,624 + BT4252 0.32 -0.4 5,603,690 + BT4252 0.37 -0.6 5,603,690 + BT4252 0.37 -0.0 5,603,691 - BT4252 0.37 -1.6 5,603,783 - BT4252 0.44 -1.6 5,603,861 + BT4252 0.51 -1.0 5,603,862 - BT4252 0.51 -0.7 5,603,926 + BT4252 0.56 -1.3 5,603,926 + BT4252 0.56 -1.0 5,603,949 - BT4252 0.58 -1.2 5,603,950 + BT4252 0.58 -2.3 5,603,950 + BT4252 0.58 -2.3 5,603,970 + BT4252 0.59 +0.6 5,604,009 - BT4252 0.62 -1.6 5,604,029 - BT4252 0.64 -2.0 5,604,050 + BT4252 0.66 -2.8 5,604,050 + BT4252 0.66 -3.1 5,604,129 - BT4252 0.72 -1.3 5,604,132 + BT4252 0.72 -2.3 5,604,132 + BT4252 0.72 -0.4 5,604,132 + BT4252 0.72 -2.0 5,604,133 - BT4252 0.72 -1.0 5,604,133 - BT4252 0.72 +1.3 5,604,133 - BT4252 0.72 -2.8 5,604,157 + BT4252 0.74 -2.3 5,604,157 + BT4252 0.74 -1.0 5,604,192 - BT4252 0.77 +0.6 5,604,256 + BT4252 0.82 -1.6 5,604,257 - BT4252 0.82 -1.4 5,604,257 - BT4252 0.82 -1.6 5,604,384 + -1.6 5,604,385 - -0.0 5,604,385 - -1.6 5,604,406 - -0.4 5,604,453 + -3.2 5,604,496 + -0.0 5,604,496 + -1.0 5,604,496 + -1.0 5,605,342 - -3.6 5,605,342 - +0.3 5,605,358 - -1.6 5,605,400 - -0.0 5,605,405 - -0.3 5,605,407 - -0.4 5,605,422 - -0.4
Or see this region's nucleotide sequence