Experiment: Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2536 and BT2537 are separated by 105 nucleotides BT2537 and BT2538 are separated by 339 nucleotides BT2538 and BT2539 are separated by 103 nucleotides BT2539 and BT2540 are separated by 221 nucleotides
BT2536: BT2536 - hypothetical protein (NCBI ptt file), at 3,170,262 to 3,170,750
BT2536
BT2537: BT2537 - hypothetical protein (NCBI ptt file), at 3,170,856 to 3,171,239
BT2537
BT2538: BT2538 - hypothetical protein (NCBI ptt file), at 3,171,579 to 3,172,061
BT2538
BT2539: BT2539 - rubredoxin (NCBI ptt file), at 3,172,165 to 3,172,329
BT2539
BT2540: BT2540 - two-component system sensor histidine kinase (NCBI ptt file), at 3,172,551 to 3,173,726
BT2540
Position (kb)
3171
3172
3173 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3170.605 kb on + strand, within BT2536 at 3170.606 kb on - strand, within BT2536 at 3170.624 kb on - strand, within BT2536 at 3170.664 kb on + strand, within BT2536 at 3170.694 kb on + strand, within BT2536 at 3170.706 kb on - strand at 3170.709 kb on + strand at 3170.710 kb on - strand at 3170.710 kb on - strand at 3170.710 kb on - strand at 3170.720 kb on + strand at 3170.721 kb on - strand at 3170.782 kb on + strand at 3170.782 kb on + strand at 3170.783 kb on - strand at 3170.843 kb on + strand at 3170.844 kb on - strand at 3170.844 kb on - strand at 3170.846 kb on - strand at 3170.847 kb on + strand at 3170.847 kb on + strand at 3170.848 kb on - strand at 3170.853 kb on + strand at 3170.858 kb on - strand at 3170.858 kb on - strand at 3170.945 kb on - strand, within BT2537 at 3170.981 kb on + strand, within BT2537 at 3170.981 kb on + strand, within BT2537 at 3171.044 kb on + strand, within BT2537 at 3171.044 kb on + strand, within BT2537 at 3171.045 kb on - strand, within BT2537 at 3171.047 kb on + strand, within BT2537 at 3171.057 kb on + strand, within BT2537 at 3171.058 kb on - strand, within BT2537 at 3171.217 kb on - strand at 3171.225 kb on + strand at 3171.244 kb on + strand at 3171.244 kb on + strand at 3171.244 kb on + strand at 3171.245 kb on - strand at 3171.245 kb on - strand at 3171.273 kb on - strand at 3171.361 kb on + strand at 3171.369 kb on + strand at 3171.369 kb on + strand at 3171.370 kb on - strand at 3171.375 kb on + strand at 3171.375 kb on + strand at 3171.380 kb on + strand at 3171.508 kb on + strand at 3171.512 kb on + strand at 3171.513 kb on - strand at 3171.587 kb on + strand at 3171.588 kb on - strand at 3171.591 kb on + strand at 3171.646 kb on + strand, within BT2538 at 3171.669 kb on - strand, within BT2538 at 3171.770 kb on + strand, within BT2538 at 3171.771 kb on - strand, within BT2538 at 3171.771 kb on - strand, within BT2538 at 3171.913 kb on - strand, within BT2538 at 3171.995 kb on + strand, within BT2538 at 3171.996 kb on - strand, within BT2538 at 3171.996 kb on - strand, within BT2538 at 3172.057 kb on + strand at 3172.137 kb on + strand at 3172.175 kb on + strand at 3172.292 kb on + strand, within BT2539 at 3172.292 kb on + strand, within BT2539 at 3172.293 kb on - strand, within BT2539 at 3172.360 kb on - strand at 3172.363 kb on - strand at 3172.433 kb on + strand at 3172.434 kb on - strand at 3172.468 kb on + strand at 3172.635 kb on + strand at 3172.638 kb on - strand at 3172.638 kb on - strand at 3172.638 kb on - strand at 3172.643 kb on - strand at 3172.648 kb on + strand at 3172.649 kb on - strand at 3172.649 kb on - strand at 3172.649 kb on - strand at 3172.660 kb on + strand at 3172.680 kb on + strand, within BT2540 at 3172.684 kb on - strand, within BT2540 at 3172.736 kb on - strand, within BT2540 at 3172.736 kb on - strand, within BT2540 at 3172.778 kb on + strand, within BT2540 at 3172.808 kb on - strand, within BT2540 at 3172.881 kb on - strand, within BT2540 at 3172.915 kb on + strand, within BT2540 at 3172.919 kb on - strand, within BT2540 at 3172.929 kb on + strand, within BT2540 at 3172.955 kb on + strand, within BT2540 at 3172.955 kb on + strand, within BT2540 at 3172.955 kb on + strand, within BT2540 at 3172.956 kb on - strand, within BT2540 at 3172.977 kb on - strand, within BT2540
Per-strain Table
Position Strand Gene LocusTag Fraction Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C) remove 3,170,605 + BT2536 0.70 -2.5 3,170,606 - BT2536 0.70 -2.6 3,170,624 - BT2536 0.74 -0.6 3,170,664 + BT2536 0.82 -0.3 3,170,694 + BT2536 0.88 +0.5 3,170,706 - -1.2 3,170,709 + -1.9 3,170,710 - +0.5 3,170,710 - -1.9 3,170,710 - -0.1 3,170,720 + -1.6 3,170,721 - +1.5 3,170,782 + +0.3 3,170,782 + -2.4 3,170,783 - -1.9 3,170,843 + -0.1 3,170,844 - -1.6 3,170,844 - -0.7 3,170,846 - -3.3 3,170,847 + +0.8 3,170,847 + -2.5 3,170,848 - -2.7 3,170,853 + -3.0 3,170,858 - -2.7 3,170,858 - +0.4 3,170,945 - BT2537 0.23 +0.1 3,170,981 + BT2537 0.33 -0.5 3,170,981 + BT2537 0.33 -1.6 3,171,044 + BT2537 0.49 -2.1 3,171,044 + BT2537 0.49 -0.6 3,171,045 - BT2537 0.49 +0.9 3,171,047 + BT2537 0.50 -0.1 3,171,057 + BT2537 0.52 +0.2 3,171,058 - BT2537 0.53 -2.1 3,171,217 - -1.1 3,171,225 + -2.9 3,171,244 + -2.4 3,171,244 + -3.2 3,171,244 + -1.1 3,171,245 - -1.1 3,171,245 - -2.1 3,171,273 - +1.9 3,171,361 + +0.9 3,171,369 + -1.4 3,171,369 + -1.1 3,171,370 - -1.5 3,171,375 + -1.1 3,171,375 + +0.9 3,171,380 + -1.9 3,171,508 + -2.6 3,171,512 + -0.8 3,171,513 - -2.2 3,171,587 + -2.1 3,171,588 - -2.4 3,171,591 + -2.6 3,171,646 + BT2538 0.14 -3.4 3,171,669 - BT2538 0.19 -1.5 3,171,770 + BT2538 0.40 -1.3 3,171,771 - BT2538 0.40 -1.3 3,171,771 - BT2538 0.40 -2.1 3,171,913 - BT2538 0.69 -1.5 3,171,995 + BT2538 0.86 -2.5 3,171,996 - BT2538 0.86 +0.4 3,171,996 - BT2538 0.86 -2.8 3,172,057 + -1.7 3,172,137 + -1.1 3,172,175 + -2.4 3,172,292 + BT2539 0.77 -3.1 3,172,292 + BT2539 0.77 -1.6 3,172,293 - BT2539 0.78 -0.3 3,172,360 - -1.1 3,172,363 - -2.4 3,172,433 + -0.6 3,172,434 - -0.1 3,172,468 + -0.1 3,172,635 + -0.1 3,172,638 - -1.1 3,172,638 - -0.1 3,172,638 - -3.2 3,172,643 - +0.9 3,172,648 + +0.5 3,172,649 - -2.4 3,172,649 - -2.6 3,172,649 - +1.5 3,172,660 + -1.9 3,172,680 + BT2540 0.11 +0.9 3,172,684 - BT2540 0.11 -3.7 3,172,736 - BT2540 0.16 -1.1 3,172,736 - BT2540 0.16 -2.4 3,172,778 + BT2540 0.19 -2.4 3,172,808 - BT2540 0.22 -2.8 3,172,881 - BT2540 0.28 +0.9 3,172,915 + BT2540 0.31 -0.1 3,172,919 - BT2540 0.31 +0.9 3,172,929 + BT2540 0.32 -1.3 3,172,955 + BT2540 0.34 -3.3 3,172,955 + BT2540 0.34 -0.2 3,172,955 + BT2540 0.34 +0.5 3,172,956 - BT2540 0.34 -2.5 3,172,977 - BT2540 0.36 +0.9
Or see this region's nucleotide sequence