Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotides BWI76_RS10435 and BWI76_RS10440 are separated by 7 nucleotides BWI76_RS10440 and BWI76_RS10445 are separated by 46 nucleotides BWI76_RS10445 and BWI76_RS10450 are separated by 239 nucleotides
BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635
_RS10430
BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402
_RS10435
BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434
_RS10440
BWI76_RS10445: BWI76_RS10445 - hypothetical protein, at 2,208,481 to 2,208,861
_RS10445
BWI76_RS10450: BWI76_RS10450 - tRNA-Tyr, at 2,209,101 to 2,209,175
_RS10450
Position (kb)
2206
2207
2208
2209 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2205.445 kb on + strand, within BWI76_RS10430 at 2205.491 kb on - strand, within BWI76_RS10430 at 2205.589 kb on - strand at 2205.739 kb on - strand at 2205.802 kb on + strand, within BWI76_RS10435 at 2206.100 kb on + strand, within BWI76_RS10435 at 2206.108 kb on - strand, within BWI76_RS10435 at 2206.505 kb on + strand at 2206.705 kb on + strand, within BWI76_RS10440 at 2206.789 kb on + strand, within BWI76_RS10440 at 2207.397 kb on - strand, within BWI76_RS10440 at 2207.489 kb on + strand, within BWI76_RS10440 at 2207.570 kb on + strand, within BWI76_RS10440 at 2207.661 kb on - strand, within BWI76_RS10440 at 2207.890 kb on + strand, within BWI76_RS10440 at 2207.993 kb on + strand, within BWI76_RS10440 at 2208.134 kb on - strand, within BWI76_RS10440 at 2208.164 kb on + strand, within BWI76_RS10440 at 2208.269 kb on + strand at 2208.282 kb on - strand at 2208.357 kb on - strand at 2208.532 kb on + strand, within BWI76_RS10445 at 2208.701 kb on - strand, within BWI76_RS10445 at 2208.741 kb on - strand, within BWI76_RS10445 at 2208.846 kb on + strand at 2208.854 kb on - strand at 2208.953 kb on + strand at 2208.953 kb on + strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.963 kb on + strand at 2209.081 kb on - strand at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.145 kb on - strand at 2209.145 kb on - strand, within BWI76_RS10450 at 2209.152 kb on + strand, within BWI76_RS10450 at 2209.190 kb on + strand at 2209.237 kb on + strand at 2209.245 kb on - strand at 2209.326 kb on + strand at 2209.334 kb on - strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.347 kb on + strand at 2209.355 kb on - strand at 2209.395 kb on + strand at 2209.402 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 2,205,445 + BWI76_RS10430 0.75 +0.3 2,205,491 - BWI76_RS10430 0.81 -0.5 2,205,589 - -0.3 2,205,739 - -0.7 2,205,802 + BWI76_RS10435 0.17 -0.2 2,206,100 + BWI76_RS10435 0.58 +0.1 2,206,108 - BWI76_RS10435 0.59 +0.5 2,206,505 + -0.2 2,206,705 + BWI76_RS10440 0.15 -1.5 2,206,789 + BWI76_RS10440 0.19 -0.6 2,207,397 - BWI76_RS10440 0.49 -1.2 2,207,489 + BWI76_RS10440 0.53 -0.2 2,207,570 + BWI76_RS10440 0.57 -0.6 2,207,661 - BWI76_RS10440 0.62 -0.1 2,207,890 + BWI76_RS10440 0.73 +0.4 2,207,993 + BWI76_RS10440 0.78 -1.2 2,208,134 - BWI76_RS10440 0.85 -0.9 2,208,164 + BWI76_RS10440 0.87 -0.1 2,208,269 + +0.4 2,208,282 - +0.1 2,208,357 - +0.7 2,208,532 + BWI76_RS10445 0.13 -0.7 2,208,701 - BWI76_RS10445 0.58 +0.4 2,208,741 - BWI76_RS10445 0.68 -0.0 2,208,846 + -0.1 2,208,854 - +0.0 2,208,953 + -0.6 2,208,953 + -0.4 2,208,961 - -0.4 2,208,961 - -0.5 2,208,961 - +2.2 2,208,963 + -1.2 2,209,081 - -1.6 2,209,112 - BWI76_RS10450 0.15 -1.3 2,209,112 - BWI76_RS10450 0.15 +0.7 2,209,145 - -0.1 2,209,145 - BWI76_RS10450 0.59 +2.4 2,209,152 + BWI76_RS10450 0.68 -0.2 2,209,190 + -0.9 2,209,237 + -0.8 2,209,245 - -1.8 2,209,326 + +0.4 2,209,334 - +0.1 2,209,337 + +0.4 2,209,337 + -0.4 2,209,337 + -0.3 2,209,337 + -0.3 2,209,345 - +0.3 2,209,345 - -0.7 2,209,345 - +1.2 2,209,345 - +1.8 2,209,347 + -0.7 2,209,355 - -0.3 2,209,395 + -0.7 2,209,402 - +0.3
Or see this region's nucleotide sequence