Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05575 and BWI76_RS05580 overlap by 4 nucleotides BWI76_RS05580 and BWI76_RS05585 overlap by 20 nucleotides
BWI76_RS05575: BWI76_RS05575 - group 1 glycosyl transferase, at 1,166,999 to 1,168,114
_RS05575
BWI76_RS05580: BWI76_RS05580 - group 1 glycosyl transferase, at 1,168,111 to 1,169,325
_RS05580
BWI76_RS05585: BWI76_RS05585 - family 2 glycosyl transferase, at 1,169,306 to 1,170,331
_RS05585
BWI76_RS05590: BWI76_RS05590 - hypothetical protein, at 1,170,324 to 1,171,706
_RS05590
Position (kb)
1168
1169
1170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1167.207 kb on - strand, within BWI76_RS05575 at 1167.224 kb on + strand, within BWI76_RS05575 at 1167.225 kb on + strand, within BWI76_RS05575 at 1167.250 kb on - strand, within BWI76_RS05575 at 1167.316 kb on + strand, within BWI76_RS05575 at 1167.591 kb on + strand, within BWI76_RS05575 at 1167.599 kb on - strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.819 kb on + strand, within BWI76_RS05575 at 1167.822 kb on - strand, within BWI76_RS05575 at 1167.952 kb on - strand, within BWI76_RS05575 at 1168.096 kb on + strand at 1168.096 kb on - strand at 1168.109 kb on - strand at 1168.250 kb on + strand, within BWI76_RS05580 at 1168.425 kb on - strand, within BWI76_RS05580 at 1168.549 kb on - strand, within BWI76_RS05580 at 1168.808 kb on + strand, within BWI76_RS05580 at 1168.918 kb on + strand, within BWI76_RS05580 at 1168.978 kb on + strand, within BWI76_RS05580 at 1168.998 kb on + strand, within BWI76_RS05580 at 1169.078 kb on + strand, within BWI76_RS05580 at 1169.114 kb on + strand, within BWI76_RS05580 at 1169.122 kb on - strand, within BWI76_RS05580 at 1169.151 kb on + strand, within BWI76_RS05580 at 1169.151 kb on + strand, within BWI76_RS05580 at 1169.159 kb on - strand, within BWI76_RS05580 at 1169.180 kb on - strand, within BWI76_RS05580 at 1169.188 kb on + strand at 1169.226 kb on + strand at 1169.234 kb on - strand at 1169.234 kb on - strand at 1169.235 kb on - strand at 1169.235 kb on - strand at 1169.239 kb on + strand at 1169.355 kb on - strand at 1169.356 kb on - strand at 1169.364 kb on - strand at 1169.374 kb on - strand at 1169.393 kb on + strand at 1169.395 kb on + strand at 1169.399 kb on - strand at 1169.401 kb on - strand at 1169.401 kb on - strand at 1169.401 kb on - strand at 1169.410 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.416 kb on - strand, within BWI76_RS05585 at 1169.447 kb on + strand, within BWI76_RS05585 at 1169.454 kb on - strand, within BWI76_RS05585 at 1169.455 kb on - strand, within BWI76_RS05585 at 1169.455 kb on - strand, within BWI76_RS05585 at 1169.490 kb on - strand, within BWI76_RS05585 at 1169.505 kb on + strand, within BWI76_RS05585 at 1169.568 kb on + strand, within BWI76_RS05585 at 1169.593 kb on - strand, within BWI76_RS05585 at 1169.632 kb on + strand, within BWI76_RS05585 at 1169.653 kb on + strand, within BWI76_RS05585 at 1169.713 kb on + strand, within BWI76_RS05585 at 1169.726 kb on - strand, within BWI76_RS05585 at 1169.800 kb on - strand, within BWI76_RS05585 at 1169.803 kb on + strand, within BWI76_RS05585 at 1169.811 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.826 kb on - strand, within BWI76_RS05585 at 1169.861 kb on + strand, within BWI76_RS05585 at 1169.892 kb on + strand, within BWI76_RS05585 at 1169.900 kb on - strand, within BWI76_RS05585 at 1169.963 kb on - strand, within BWI76_RS05585 at 1170.022 kb on - strand, within BWI76_RS05585 at 1170.109 kb on + strand, within BWI76_RS05585 at 1170.228 kb on + strand, within BWI76_RS05585 at 1170.228 kb on + strand, within BWI76_RS05585 at 1170.236 kb on - strand at 1170.275 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 1,167,207 - BWI76_RS05575 0.19 +0.4 1,167,224 + BWI76_RS05575 0.20 -0.6 1,167,225 + BWI76_RS05575 0.20 -0.5 1,167,250 - BWI76_RS05575 0.22 -1.7 1,167,316 + BWI76_RS05575 0.28 +0.2 1,167,591 + BWI76_RS05575 0.53 -1.1 1,167,599 - BWI76_RS05575 0.54 -0.5 1,167,814 + BWI76_RS05575 0.73 +0.0 1,167,814 + BWI76_RS05575 0.73 -0.2 1,167,819 + BWI76_RS05575 0.73 +0.3 1,167,822 - BWI76_RS05575 0.74 +0.2 1,167,952 - BWI76_RS05575 0.85 +0.4 1,168,096 + -1.0 1,168,096 - -0.6 1,168,109 - -0.4 1,168,250 + BWI76_RS05580 0.11 +4.5 1,168,425 - BWI76_RS05580 0.26 -0.6 1,168,549 - BWI76_RS05580 0.36 +0.2 1,168,808 + BWI76_RS05580 0.57 +1.6 1,168,918 + BWI76_RS05580 0.66 +1.2 1,168,978 + BWI76_RS05580 0.71 +0.7 1,168,998 + BWI76_RS05580 0.73 +0.5 1,169,078 + BWI76_RS05580 0.80 +0.8 1,169,114 + BWI76_RS05580 0.83 -0.4 1,169,122 - BWI76_RS05580 0.83 -0.4 1,169,151 + BWI76_RS05580 0.86 -0.4 1,169,151 + BWI76_RS05580 0.86 +0.8 1,169,159 - BWI76_RS05580 0.86 +1.3 1,169,180 - BWI76_RS05580 0.88 +3.7 1,169,188 + +1.9 1,169,226 + -0.6 1,169,234 - +0.6 1,169,234 - +0.0 1,169,235 - +0.3 1,169,235 - -2.3 1,169,239 + -1.5 1,169,355 - -0.6 1,169,356 - -3.4 1,169,364 - -0.5 1,169,374 - +0.6 1,169,393 + +0.9 1,169,395 + -1.2 1,169,399 - +0.2 1,169,401 - +0.0 1,169,401 - -0.2 1,169,401 - -1.9 1,169,410 - BWI76_RS05585 0.10 -0.2 1,169,416 - BWI76_RS05585 0.11 +2.3 1,169,416 - BWI76_RS05585 0.11 -0.3 1,169,416 - BWI76_RS05585 0.11 +0.2 1,169,416 - BWI76_RS05585 0.11 +1.1 1,169,416 - BWI76_RS05585 0.11 +0.2 1,169,447 + BWI76_RS05585 0.14 +1.1 1,169,454 - BWI76_RS05585 0.14 +0.0 1,169,455 - BWI76_RS05585 0.15 -1.1 1,169,455 - BWI76_RS05585 0.15 -0.9 1,169,490 - BWI76_RS05585 0.18 +0.7 1,169,505 + BWI76_RS05585 0.19 +0.1 1,169,568 + BWI76_RS05585 0.26 -1.8 1,169,593 - BWI76_RS05585 0.28 -0.1 1,169,632 + BWI76_RS05585 0.32 -0.8 1,169,653 + BWI76_RS05585 0.34 -0.7 1,169,713 + BWI76_RS05585 0.40 +0.2 1,169,726 - BWI76_RS05585 0.41 -0.8 1,169,800 - BWI76_RS05585 0.48 -0.5 1,169,803 + BWI76_RS05585 0.48 +0.9 1,169,811 - BWI76_RS05585 0.49 -0.4 1,169,826 - BWI76_RS05585 0.51 -0.5 1,169,826 - BWI76_RS05585 0.51 +0.0 1,169,826 - BWI76_RS05585 0.51 +0.2 1,169,861 + BWI76_RS05585 0.54 -2.4 1,169,892 + BWI76_RS05585 0.57 +0.6 1,169,900 - BWI76_RS05585 0.58 -2.0 1,169,963 - BWI76_RS05585 0.64 -1.1 1,170,022 - BWI76_RS05585 0.70 +0.1 1,170,109 + BWI76_RS05585 0.78 +1.6 1,170,228 + BWI76_RS05585 0.90 -0.8 1,170,228 + BWI76_RS05585 0.90 +0.3 1,170,236 - -0.2 1,170,275 + -1.2
Or see this region's nucleotide sequence