Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12240 and BWI76_RS12245 are separated by 104 nucleotides BWI76_RS12245 and BWI76_RS12250 are separated by 14 nucleotides
BWI76_RS12240: BWI76_RS12240 - terminase small subunit, at 2,557,707 to 2,558,699
_RS12240
BWI76_RS12245: BWI76_RS12245 - hypothetical protein, at 2,558,804 to 2,559,706
_RS12245
BWI76_RS12250: BWI76_RS12250 - terminase, at 2,559,721 to 2,560,989
_RS12250
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2558.012 kb on - strand, within BWI76_RS12240 at 2558.021 kb on - strand, within BWI76_RS12240 at 2558.146 kb on + strand, within BWI76_RS12240 at 2558.232 kb on + strand, within BWI76_RS12240 at 2558.335 kb on - strand, within BWI76_RS12240 at 2558.356 kb on - strand, within BWI76_RS12240 at 2558.412 kb on - strand, within BWI76_RS12240 at 2558.594 kb on + strand, within BWI76_RS12240 at 2558.687 kb on + strand at 2558.774 kb on + strand at 2558.780 kb on + strand at 2558.893 kb on - strand at 2558.898 kb on + strand, within BWI76_RS12245 at 2558.906 kb on - strand, within BWI76_RS12245 at 2558.906 kb on - strand, within BWI76_RS12245 at 2559.047 kb on + strand, within BWI76_RS12245 at 2559.060 kb on - strand, within BWI76_RS12245 at 2559.073 kb on + strand, within BWI76_RS12245 at 2559.177 kb on - strand, within BWI76_RS12245 at 2559.250 kb on + strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.356 kb on - strand, within BWI76_RS12245 at 2559.508 kb on + strand, within BWI76_RS12245 at 2559.516 kb on - strand, within BWI76_RS12245 at 2559.557 kb on + strand, within BWI76_RS12245 at 2559.557 kb on + strand, within BWI76_RS12245 at 2559.634 kb on - strand at 2559.634 kb on - strand at 2559.634 kb on - strand at 2559.688 kb on - strand at 2559.823 kb on - strand at 2559.840 kb on + strand at 2559.848 kb on - strand, within BWI76_RS12250 at 2559.848 kb on - strand, within BWI76_RS12250 at 2559.983 kb on - strand, within BWI76_RS12250 at 2560.011 kb on + strand, within BWI76_RS12250 at 2560.019 kb on - strand, within BWI76_RS12250 at 2560.054 kb on + strand, within BWI76_RS12250 at 2560.175 kb on + strand, within BWI76_RS12250 at 2560.206 kb on + strand, within BWI76_RS12250 at 2560.234 kb on + strand, within BWI76_RS12250 at 2560.249 kb on + strand, within BWI76_RS12250 at 2560.314 kb on + strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.340 kb on - strand, within BWI76_RS12250 at 2560.384 kb on - strand, within BWI76_RS12250 at 2560.441 kb on + strand, within BWI76_RS12250 at 2560.469 kb on - strand, within BWI76_RS12250 at 2560.502 kb on + strand, within BWI76_RS12250 at 2560.510 kb on - strand, within BWI76_RS12250 at 2560.533 kb on + strand, within BWI76_RS12250 at 2560.540 kb on - strand, within BWI76_RS12250
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 2,558,012 - BWI76_RS12240 0.31 -1.2 2,558,021 - BWI76_RS12240 0.32 -2.3 2,558,146 + BWI76_RS12240 0.44 +0.3 2,558,232 + BWI76_RS12240 0.53 -1.6 2,558,335 - BWI76_RS12240 0.63 -1.6 2,558,356 - BWI76_RS12240 0.65 -1.0 2,558,412 - BWI76_RS12240 0.71 -2.4 2,558,594 + BWI76_RS12240 0.89 -1.3 2,558,687 + -0.6 2,558,774 + +1.4 2,558,780 + -1.2 2,558,893 - -1.1 2,558,898 + BWI76_RS12245 0.10 -1.1 2,558,906 - BWI76_RS12245 0.11 -2.1 2,558,906 - BWI76_RS12245 0.11 -1.3 2,559,047 + BWI76_RS12245 0.27 -1.1 2,559,060 - BWI76_RS12245 0.28 -0.8 2,559,073 + BWI76_RS12245 0.30 -0.2 2,559,177 - BWI76_RS12245 0.41 -1.3 2,559,250 + BWI76_RS12245 0.49 -2.8 2,559,356 - BWI76_RS12245 0.61 -1.2 2,559,356 - BWI76_RS12245 0.61 -1.6 2,559,356 - BWI76_RS12245 0.61 -0.9 2,559,356 - BWI76_RS12245 0.61 -2.1 2,559,508 + BWI76_RS12245 0.78 -1.7 2,559,516 - BWI76_RS12245 0.79 -1.4 2,559,557 + BWI76_RS12245 0.83 -1.9 2,559,557 + BWI76_RS12245 0.83 +0.0 2,559,634 - -1.2 2,559,634 - -1.5 2,559,634 - -1.8 2,559,688 - -1.4 2,559,823 - -0.8 2,559,840 + -0.4 2,559,848 - BWI76_RS12250 0.10 +0.2 2,559,848 - BWI76_RS12250 0.10 -0.0 2,559,983 - BWI76_RS12250 0.21 -1.6 2,560,011 + BWI76_RS12250 0.23 +0.5 2,560,019 - BWI76_RS12250 0.23 -1.4 2,560,054 + BWI76_RS12250 0.26 -1.2 2,560,175 + BWI76_RS12250 0.36 -1.6 2,560,206 + BWI76_RS12250 0.38 -1.1 2,560,234 + BWI76_RS12250 0.40 -0.9 2,560,249 + BWI76_RS12250 0.42 -0.9 2,560,314 + BWI76_RS12250 0.47 -1.7 2,560,340 - BWI76_RS12250 0.49 -2.1 2,560,340 - BWI76_RS12250 0.49 -1.6 2,560,340 - BWI76_RS12250 0.49 -2.0 2,560,384 - BWI76_RS12250 0.52 -1.2 2,560,441 + BWI76_RS12250 0.57 -3.0 2,560,469 - BWI76_RS12250 0.59 -2.1 2,560,502 + BWI76_RS12250 0.62 -0.9 2,560,510 - BWI76_RS12250 0.62 +3.4 2,560,533 + BWI76_RS12250 0.64 -1.1 2,560,540 - BWI76_RS12250 0.65 -2.1
Or see this region's nucleotide sequence