Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08210

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08205 and BWI76_RS08210 are separated by 8 nucleotidesBWI76_RS08210 and BWI76_RS08215 are separated by 60 nucleotides BWI76_RS08205: BWI76_RS08205 - DNA-binding transcriptional dual regulator NagC, at 1,735,483 to 1,736,703 _RS08205 BWI76_RS08210: BWI76_RS08210 - N-acetylglucosamine-6-phosphate deacetylase, at 1,736,712 to 1,737,860 _RS08210 BWI76_RS08215: BWI76_RS08215 - glucosamine-6-phosphate deaminase, at 1,737,921 to 1,738,721 _RS08215 Position (kb) 1736 1737 1738Strain fitness (log2 ratio) -3 -2 -1 0 1at 1735.730 kb on + strand, within BWI76_RS08205at 1735.915 kb on + strand, within BWI76_RS08205at 1736.060 kb on - strand, within BWI76_RS08205at 1736.300 kb on - strand, within BWI76_RS08205at 1736.394 kb on - strand, within BWI76_RS08205at 1736.430 kb on - strand, within BWI76_RS08205at 1737.060 kb on - strand, within BWI76_RS08210at 1737.418 kb on - strand, within BWI76_RS08210at 1738.242 kb on + strand, within BWI76_RS08215at 1738.483 kb on + strand, within BWI76_RS08215at 1738.505 kb on + strand, within BWI76_RS08215at 1738.534 kb on + strand, within BWI76_RS08215at 1738.696 kb on + strandat 1738.773 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
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1,735,730 + BWI76_RS08205 0.20 -1.9
1,735,915 + BWI76_RS08205 0.35 -1.2
1,736,060 - BWI76_RS08205 0.47 -1.8
1,736,300 - BWI76_RS08205 0.67 -3.4
1,736,394 - BWI76_RS08205 0.75 -2.8
1,736,430 - BWI76_RS08205 0.78 -3.6
1,737,060 - BWI76_RS08210 0.30 -2.3
1,737,418 - BWI76_RS08210 0.61 -2.4
1,738,242 + BWI76_RS08215 0.40 -0.2
1,738,483 + BWI76_RS08215 0.70 -0.8
1,738,505 + BWI76_RS08215 0.73 -0.2
1,738,534 + BWI76_RS08215 0.77 +1.0
1,738,696 + -0.6
1,738,773 + -0.1

Or see this region's nucleotide sequence