Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20840 and BWI76_RS20845 are separated by 78 nucleotides BWI76_RS20845 and BWI76_RS20850 are separated by 58 nucleotides BWI76_RS20850 and BWI76_RS20855 are separated by 186 nucleotides BWI76_RS20855 and BWI76_RS20860 are separated by 5 nucleotides
BWI76_RS20840: BWI76_RS20840 - hypothetical protein, at 4,356,046 to 4,357,113
_RS20840
BWI76_RS20845: BWI76_RS20845 - GDP-mannose pyrophosphatase NudK, at 4,357,192 to 4,357,776
_RS20845
BWI76_RS20850: BWI76_RS20850 - oxidoreductase FeS-binding subunit, at 4,357,835 to 4,359,799
_RS20850
BWI76_RS20855: BWI76_RS20855 - hypothetical protein, at 4,359,986 to 4,360,166
_RS20855
BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285
_RS20860
Position (kb) 4357
4358
4359
4360 Strain fitness (log2 ratio) -1
0
1 at 4356.930 kb on + strand, within BWI76_RS20840 at 4357.582 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.656 kb on - strand, within BWI76_RS20845 at 4357.807 kb on + strand at 4357.834 kb on - strand at 4358.128 kb on + strand, within BWI76_RS20850 at 4358.624 kb on - strand, within BWI76_RS20850 at 4358.896 kb on - strand, within BWI76_RS20850 at 4358.900 kb on + strand, within BWI76_RS20850 at 4359.006 kb on + strand, within BWI76_RS20850 at 4359.093 kb on + strand, within BWI76_RS20850 at 4359.150 kb on + strand, within BWI76_RS20850 at 4359.155 kb on + strand, within BWI76_RS20850 at 4359.321 kb on + strand, within BWI76_RS20850 at 4359.350 kb on - strand, within BWI76_RS20850 at 4359.636 kb on - strand at 4359.668 kb on - strand at 4359.670 kb on + strand at 4359.742 kb on + strand at 4359.983 kb on + strand at 4360.001 kb on + strand at 4360.009 kb on - strand, within BWI76_RS20855 at 4360.011 kb on + strand, within BWI76_RS20855 at 4360.013 kb on + strand, within BWI76_RS20855 at 4360.025 kb on + strand, within BWI76_RS20855 at 4360.033 kb on + strand, within BWI76_RS20855 at 4360.098 kb on + strand, within BWI76_RS20855 at 4360.100 kb on - strand, within BWI76_RS20855 at 4360.106 kb on - strand, within BWI76_RS20855 at 4360.106 kb on - strand, within BWI76_RS20855 at 4360.118 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.120 kb on + strand, within BWI76_RS20855 at 4360.128 kb on - strand, within BWI76_RS20855 at 4360.235 kb on + strand at 4360.275 kb on + strand at 4360.367 kb on + strand at 4360.372 kb on + strand at 4360.417 kb on - strand at 4360.471 kb on + strand at 4360.525 kb on + strand, within BWI76_RS20860 at 4360.583 kb on - strand, within BWI76_RS20860 at 4360.707 kb on - strand, within BWI76_RS20860 at 4360.747 kb on + strand, within BWI76_RS20860
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 4,356,930 + BWI76_RS20840 0.83 -1.0 4,357,582 - BWI76_RS20845 0.67 +0.1 4,357,656 - BWI76_RS20845 0.79 -0.2 4,357,656 - BWI76_RS20845 0.79 -0.1 4,357,807 + -0.5 4,357,834 - +0.0 4,358,128 + BWI76_RS20850 0.15 -0.3 4,358,624 - BWI76_RS20850 0.40 +0.8 4,358,896 - BWI76_RS20850 0.54 +0.4 4,358,900 + BWI76_RS20850 0.54 -0.8 4,359,006 + BWI76_RS20850 0.60 +0.4 4,359,093 + BWI76_RS20850 0.64 -0.0 4,359,150 + BWI76_RS20850 0.67 -1.4 4,359,155 + BWI76_RS20850 0.67 +0.4 4,359,321 + BWI76_RS20850 0.76 -0.4 4,359,350 - BWI76_RS20850 0.77 +0.1 4,359,636 - +0.8 4,359,668 - -1.4 4,359,670 + +0.4 4,359,742 + -0.6 4,359,983 + -0.3 4,360,001 + -0.9 4,360,009 - BWI76_RS20855 0.13 -0.3 4,360,011 + BWI76_RS20855 0.14 -0.3 4,360,013 + BWI76_RS20855 0.15 +0.3 4,360,025 + BWI76_RS20855 0.22 +0.3 4,360,033 + BWI76_RS20855 0.26 -0.8 4,360,098 + BWI76_RS20855 0.62 -0.0 4,360,100 - BWI76_RS20855 0.63 -1.0 4,360,106 - BWI76_RS20855 0.66 +0.1 4,360,106 - BWI76_RS20855 0.66 -0.5 4,360,118 + BWI76_RS20855 0.73 -0.2 4,360,120 + BWI76_RS20855 0.74 +0.3 4,360,120 + BWI76_RS20855 0.74 +1.0 4,360,120 + BWI76_RS20855 0.74 +0.4 4,360,128 - BWI76_RS20855 0.78 +0.9 4,360,235 + -0.5 4,360,275 + -0.9 4,360,367 + -0.9 4,360,372 + +0.6 4,360,417 - -0.0 4,360,471 + +0.0 4,360,525 + BWI76_RS20860 0.11 -0.5 4,360,583 - BWI76_RS20860 0.13 -0.0 4,360,707 - BWI76_RS20860 0.17 -0.2 4,360,747 + BWI76_RS20860 0.18 -0.8
Or see this region's nucleotide sequence