Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04485 and BWI76_RS04490 are separated by 166 nucleotides BWI76_RS04490 and BWI76_RS04495 overlap by 8 nucleotides
BWI76_RS04485: BWI76_RS04485 - hypothetical protein, at 928,559 to 930,115
_RS04485
BWI76_RS04490: BWI76_RS04490 - glutathione-regulated potassium-efflux system ancillary protein KefF, at 930,282 to 930,815
_RS04490
BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673
_RS04495
Position (kb) 930
931 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand, within BWI76_RS04485 at 929.284 kb on + strand at 929.287 kb on + strand, within BWI76_RS04485 at 929.307 kb on - strand, within BWI76_RS04485 at 929.328 kb on + strand, within BWI76_RS04485 at 929.329 kb on - strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.333 kb on + strand, within BWI76_RS04485 at 929.336 kb on - strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.339 kb on + strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand, within BWI76_RS04485 at 929.341 kb on - strand at 929.341 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand at 929.342 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand, within BWI76_RS04485 at 929.342 kb on - strand, within BWI76_RS04485 at 929.346 kb on + strand, within BWI76_RS04485 at 929.347 kb on - strand at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.347 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.354 kb on - strand, within BWI76_RS04485 at 929.389 kb on + strand, within BWI76_RS04485 at 929.405 kb on - strand, within BWI76_RS04485 at 929.417 kb on + strand, within BWI76_RS04485 at 929.562 kb on + strand, within BWI76_RS04485 at 929.858 kb on - strand, within BWI76_RS04485 at 929.867 kb on - strand, within BWI76_RS04485 at 929.872 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 930.037 kb on - strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.041 kb on + strand at 930.047 kb on - strand at 930.074 kb on + strand at 930.074 kb on + strand at 930.082 kb on - strand at 930.093 kb on - strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.114 kb on + strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.127 kb on - strand at 930.135 kb on - strand at 930.139 kb on + strand at 930.147 kb on - strand at 930.147 kb on - strand at 930.159 kb on + strand at 930.166 kb on + strand at 930.166 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.185 kb on + strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.215 kb on - strand at 930.243 kb on + strand at 930.244 kb on - strand at 930.245 kb on - strand at 930.251 kb on - strand at 930.253 kb on - strand at 930.259 kb on - strand at 930.263 kb on + strand at 930.264 kb on - strand at 930.264 kb on - strand at 930.270 kb on - strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.334 kb on + strand at 930.340 kb on + strand, within BWI76_RS04490 at 930.340 kb on + strand, within BWI76_RS04490 at 930.350 kb on + strand, within BWI76_RS04490 at 930.358 kb on - strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.369 kb on + strand, within BWI76_RS04490 at 930.380 kb on - strand, within BWI76_RS04490 at 930.421 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.443 kb on - strand, within BWI76_RS04490 at 930.445 kb on + strand, within BWI76_RS04490 at 930.485 kb on - strand, within BWI76_RS04490 at 930.495 kb on - strand, within BWI76_RS04490 at 930.523 kb on - strand, within BWI76_RS04490 at 930.528 kb on + strand, within BWI76_RS04490 at 930.533 kb on + strand, within BWI76_RS04490 at 930.680 kb on + strand, within BWI76_RS04490 at 930.815 kb on + strand at 930.815 kb on + strand at 930.881 kb on + strand at 930.889 kb on - strand at 930.997 kb on + strand, within BWI76_RS04495 at 931.027 kb on - strand, within BWI76_RS04495 at 931.042 kb on - strand, within BWI76_RS04495 at 931.116 kb on + strand, within BWI76_RS04495 at 931.169 kb on + strand, within BWI76_RS04495 at 931.179 kb on + strand, within BWI76_RS04495 at 931.187 kb on - strand, within BWI76_RS04495 at 931.250 kb on + strand, within BWI76_RS04495 at 931.795 kb on - strand, within BWI76_RS04495
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 929,284 + BWI76_RS04485 0.47 -0.4 929,284 + BWI76_RS04485 0.47 -0.9 929,284 + BWI76_RS04485 0.47 -0.5 929,284 + BWI76_RS04485 0.47 -0.1 929,284 + -0.3 929,287 + BWI76_RS04485 0.47 +0.9 929,307 - BWI76_RS04485 0.48 +0.1 929,328 + BWI76_RS04485 0.49 +0.2 929,329 - BWI76_RS04485 0.49 -0.3 929,333 + BWI76_RS04485 0.50 +0.5 929,333 + BWI76_RS04485 0.50 +0.3 929,333 + BWI76_RS04485 0.50 +0.3 929,333 + BWI76_RS04485 0.50 -0.3 929,333 + BWI76_RS04485 0.50 -0.3 929,336 - BWI76_RS04485 0.50 -2.1 929,339 + BWI76_RS04485 0.50 +0.8 929,339 + BWI76_RS04485 0.50 -0.3 929,339 + BWI76_RS04485 0.50 +0.2 929,339 + BWI76_RS04485 0.50 +0.6 929,339 + BWI76_RS04485 0.50 +0.0 929,341 - BWI76_RS04485 0.50 +0.7 929,341 - BWI76_RS04485 0.50 +1.6 929,341 - BWI76_RS04485 0.50 +1.2 929,341 - BWI76_RS04485 0.50 -0.3 929,341 - BWI76_RS04485 0.50 +1.8 929,341 - BWI76_RS04485 0.50 -0.2 929,341 - BWI76_RS04485 0.50 +0.3 929,341 - BWI76_RS04485 0.50 +0.6 929,341 - BWI76_RS04485 0.50 +0.1 929,341 - +0.6 929,341 - BWI76_RS04485 0.50 +0.1 929,342 - +0.6 929,342 - BWI76_RS04485 0.50 -0.2 929,342 - BWI76_RS04485 0.50 +0.2 929,342 - BWI76_RS04485 0.50 -2.0 929,346 + BWI76_RS04485 0.51 -0.3 929,347 - -0.2 929,347 - BWI76_RS04485 0.51 -0.0 929,347 - BWI76_RS04485 0.51 +0.8 929,347 - BWI76_RS04485 0.51 +0.6 929,347 - BWI76_RS04485 0.51 +1.3 929,347 - BWI76_RS04485 0.51 +0.9 929,347 - BWI76_RS04485 0.51 +0.2 929,347 - BWI76_RS04485 0.51 -0.2 929,347 - BWI76_RS04485 0.51 +0.3 929,347 - BWI76_RS04485 0.51 -1.1 929,354 - BWI76_RS04485 0.51 -0.7 929,354 - BWI76_RS04485 0.51 -3.1 929,354 - BWI76_RS04485 0.51 -0.1 929,389 + BWI76_RS04485 0.53 +0.6 929,405 - BWI76_RS04485 0.54 -0.5 929,417 + BWI76_RS04485 0.55 +1.1 929,562 + BWI76_RS04485 0.64 -0.6 929,858 - BWI76_RS04485 0.83 -0.2 929,867 - BWI76_RS04485 0.84 -0.6 929,872 + BWI76_RS04485 0.84 -0.6 929,904 + BWI76_RS04485 0.86 +1.8 929,904 + BWI76_RS04485 0.86 +0.3 929,904 + BWI76_RS04485 0.86 -0.3 929,904 + BWI76_RS04485 0.86 -0.9 929,904 + BWI76_RS04485 0.86 +0.3 929,904 + BWI76_RS04485 0.86 -2.9 929,904 + BWI76_RS04485 0.86 -0.6 929,912 - BWI76_RS04485 0.87 -1.1 929,912 - BWI76_RS04485 0.87 -1.2 929,912 - BWI76_RS04485 0.87 -2.1 929,912 - BWI76_RS04485 0.87 -2.0 929,912 - BWI76_RS04485 0.87 +0.3 929,912 - BWI76_RS04485 0.87 -1.2 929,958 + BWI76_RS04485 0.90 -0.0 929,958 + BWI76_RS04485 0.90 -0.2 929,959 + BWI76_RS04485 0.90 +2.4 929,959 + BWI76_RS04485 0.90 +0.0 929,959 + BWI76_RS04485 0.90 +3.0 929,959 + BWI76_RS04485 0.90 -0.4 929,959 + BWI76_RS04485 0.90 -0.8 929,967 - +1.0 929,967 - +0.0 929,967 - -0.3 929,967 - -0.4 929,967 - -0.8 929,967 - +3.7 929,967 - -0.7 930,037 - -1.0 930,039 + +0.3 930,039 + -1.0 930,039 + +1.8 930,039 + +0.0 930,039 + -0.8 930,039 + +0.0 930,039 + +1.1 930,039 + -1.3 930,039 + -0.0 930,041 + -0.8 930,047 - +0.7 930,074 + +0.1 930,074 + -1.5 930,082 - -0.0 930,093 - -2.1 930,109 + -0.8 930,109 + -1.4 930,109 + -0.2 930,109 + +2.0 930,109 + +2.4 930,109 + -0.4 930,109 + -0.2 930,109 + +0.8 930,114 + -0.5 930,117 - -2.4 930,117 - -0.7 930,117 - +0.3 930,117 - -1.7 930,119 + -0.2 930,119 + +0.5 930,119 + +0.2 930,119 + -1.0 930,119 + -0.6 930,127 - -0.4 930,135 - +0.3 930,139 + -0.1 930,147 - -0.8 930,147 - +0.7 930,159 + +0.4 930,166 + +0.2 930,166 + -0.1 930,174 + +0.3 930,174 + -0.2 930,174 + -0.7 930,174 + +0.2 930,174 - -0.5 930,182 - -1.0 930,182 - -1.7 930,182 - -1.7 930,182 - -0.2 930,182 - +0.0 930,182 - +0.9 930,182 - -0.2 930,182 - +2.2 930,182 - +0.1 930,182 - +1.9 930,182 - -0.5 930,182 - -1.2 930,182 - +1.0 930,182 - -0.3 930,184 + -0.2 930,184 + -0.2 930,184 + -1.8 930,185 + +0.3 930,192 - -0.2 930,192 - -0.5 930,192 - -0.8 930,192 - -0.3 930,192 - -0.2 930,192 - -0.3 930,215 - +1.7 930,243 + -0.6 930,244 - +0.8 930,245 - -0.4 930,251 - -0.2 930,253 - -0.0 930,259 - -0.8 930,263 + +0.5 930,264 - -0.7 930,264 - +1.1 930,270 - +1.4 930,305 + -0.9 930,305 + -0.1 930,305 + -0.8 930,305 + -2.2 930,305 + -0.2 930,313 - -0.5 930,313 - +0.6 930,313 - -0.0 930,334 + -0.8 930,340 + BWI76_RS04490 0.11 +0.1 930,340 + BWI76_RS04490 0.11 -2.3 930,350 + BWI76_RS04490 0.13 -0.4 930,358 - BWI76_RS04490 0.14 -0.2 930,367 + BWI76_RS04490 0.16 -0.9 930,367 + BWI76_RS04490 0.16 -1.5 930,369 + BWI76_RS04490 0.16 -0.4 930,380 - BWI76_RS04490 0.18 -0.0 930,421 + BWI76_RS04490 0.26 +0.4 930,435 + BWI76_RS04490 0.29 -0.4 930,435 + BWI76_RS04490 0.29 +0.2 930,435 + BWI76_RS04490 0.29 -1.2 930,443 - BWI76_RS04490 0.30 -1.5 930,445 + BWI76_RS04490 0.31 -0.2 930,485 - BWI76_RS04490 0.38 -0.8 930,495 - BWI76_RS04490 0.40 -1.2 930,523 - BWI76_RS04490 0.45 -0.4 930,528 + BWI76_RS04490 0.46 -0.4 930,533 + BWI76_RS04490 0.47 -0.7 930,680 + BWI76_RS04490 0.75 +2.0 930,815 + +0.1 930,815 + -2.3 930,881 + -0.0 930,889 - +0.0 930,997 + BWI76_RS04495 0.10 -0.4 931,027 - BWI76_RS04495 0.12 +1.1 931,042 - BWI76_RS04495 0.13 +0.0 931,116 + BWI76_RS04495 0.17 -0.4 931,169 + BWI76_RS04495 0.19 -0.9 931,179 + BWI76_RS04495 0.20 -0.8 931,187 - BWI76_RS04495 0.20 -0.0 931,250 + BWI76_RS04495 0.24 -0.5 931,795 - BWI76_RS04495 0.53 +2.8
Or see this region's nucleotide sequence