Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02315 and BWI76_RS02320 overlap by 25 nucleotides BWI76_RS02320 and BWI76_RS02325 are separated by 94 nucleotides BWI76_RS02325 and BWI76_RS02330 are separated by 118 nucleotides
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
BWI76_RS02320: BWI76_RS02320 - divalent ion tolerance protein CutA, at 477,576 to 477,899
_RS02320
BWI76_RS02325: BWI76_RS02325 - anaerobic C4-dicarboxylate transporter, at 477,994 to 479,295
_RS02325
BWI76_RS02330: BWI76_RS02330 - aspartate ammonia-lyase, at 479,414 to 480,850
_RS02330
Position (kb) 477
478
479
480 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 477.006 kb on - strand, within BWI76_RS02315 at 477.252 kb on + strand, within BWI76_RS02315 at 477.353 kb on - strand, within BWI76_RS02315 at 477.423 kb on + strand, within BWI76_RS02315 at 477.453 kb on + strand at 477.461 kb on - strand at 477.573 kb on - strand at 477.681 kb on + strand, within BWI76_RS02320 at 477.793 kb on - strand, within BWI76_RS02320 at 477.837 kb on + strand, within BWI76_RS02320 at 477.844 kb on - strand, within BWI76_RS02320 at 477.860 kb on + strand, within BWI76_RS02320 at 477.868 kb on - strand at 477.881 kb on - strand at 477.929 kb on + strand at 477.972 kb on - strand at 478.046 kb on - strand at 478.060 kb on + strand at 478.098 kb on + strand at 478.201 kb on - strand, within BWI76_RS02325 at 478.211 kb on - strand, within BWI76_RS02325 at 478.336 kb on + strand, within BWI76_RS02325 at 478.346 kb on + strand, within BWI76_RS02325 at 478.472 kb on + strand, within BWI76_RS02325 at 478.480 kb on - strand, within BWI76_RS02325 at 478.538 kb on - strand, within BWI76_RS02325 at 478.543 kb on + strand, within BWI76_RS02325 at 478.548 kb on + strand, within BWI76_RS02325 at 478.556 kb on - strand, within BWI76_RS02325 at 478.617 kb on - strand, within BWI76_RS02325 at 478.671 kb on - strand, within BWI76_RS02325 at 478.677 kb on - strand, within BWI76_RS02325 at 478.794 kb on + strand, within BWI76_RS02325 at 478.949 kb on - strand, within BWI76_RS02325 at 479.072 kb on - strand, within BWI76_RS02325 at 479.137 kb on + strand, within BWI76_RS02325 at 479.165 kb on - strand at 479.210 kb on - strand at 479.288 kb on - strand at 479.293 kb on + strand at 479.293 kb on - strand at 479.302 kb on - strand at 479.395 kb on - strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.433 kb on + strand at 479.441 kb on - strand at 479.476 kb on - strand at 479.502 kb on - strand at 479.523 kb on + strand at 479.588 kb on - strand, within BWI76_RS02330 at 479.610 kb on - strand, within BWI76_RS02330 at 479.889 kb on - strand, within BWI76_RS02330 at 479.970 kb on + strand, within BWI76_RS02330 at 480.014 kb on - strand, within BWI76_RS02330 at 480.112 kb on + strand, within BWI76_RS02330 at 480.120 kb on - strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.131 kb on + strand, within BWI76_RS02330 at 480.139 kb on - strand, within BWI76_RS02330
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 477,006 - BWI76_RS02315 0.66 +0.9 477,252 + BWI76_RS02315 0.80 -1.3 477,353 - BWI76_RS02315 0.86 -0.2 477,423 + BWI76_RS02315 0.90 +0.1 477,453 + -0.4 477,461 - -0.2 477,573 - +0.1 477,681 + BWI76_RS02320 0.32 +0.4 477,793 - BWI76_RS02320 0.67 +0.9 477,837 + BWI76_RS02320 0.81 -0.3 477,844 - BWI76_RS02320 0.83 -1.5 477,860 + BWI76_RS02320 0.88 +0.2 477,868 - -0.9 477,881 - -0.0 477,929 + -2.3 477,972 - -1.0 478,046 - +0.5 478,060 + -0.2 478,098 + -0.2 478,201 - BWI76_RS02325 0.16 -0.4 478,211 - BWI76_RS02325 0.17 -0.6 478,336 + BWI76_RS02325 0.26 +1.6 478,346 + BWI76_RS02325 0.27 -1.3 478,472 + BWI76_RS02325 0.37 -0.4 478,480 - BWI76_RS02325 0.37 -0.4 478,538 - BWI76_RS02325 0.42 +0.3 478,543 + BWI76_RS02325 0.42 +0.0 478,548 + BWI76_RS02325 0.43 +4.6 478,556 - BWI76_RS02325 0.43 +0.4 478,617 - BWI76_RS02325 0.48 +0.0 478,671 - BWI76_RS02325 0.52 +0.9 478,677 - BWI76_RS02325 0.52 -1.3 478,794 + BWI76_RS02325 0.61 +1.4 478,949 - BWI76_RS02325 0.73 -0.0 479,072 - BWI76_RS02325 0.83 -0.9 479,137 + BWI76_RS02325 0.88 -0.6 479,165 - -0.4 479,210 - +0.1 479,288 - -0.4 479,293 + +0.0 479,293 - -0.5 479,302 - -1.1 479,395 - -0.1 479,433 + +0.0 479,433 + +0.2 479,433 + -0.5 479,433 + -0.2 479,441 - -1.7 479,476 - +1.1 479,502 - -0.7 479,523 + -0.5 479,588 - BWI76_RS02330 0.12 -0.9 479,610 - BWI76_RS02330 0.14 -1.9 479,889 - BWI76_RS02330 0.33 -0.2 479,970 + BWI76_RS02330 0.39 -0.3 480,014 - BWI76_RS02330 0.42 -0.1 480,112 + BWI76_RS02330 0.49 -1.2 480,120 - BWI76_RS02330 0.49 -0.5 480,131 + BWI76_RS02330 0.50 -0.4 480,131 + BWI76_RS02330 0.50 +0.4 480,139 - BWI76_RS02330 0.50 -0.5
Or see this region's nucleotide sequence