Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17180 and BWI76_RS17185 are separated by 106 nucleotides BWI76_RS17185 and BWI76_RS17190 are separated by 19 nucleotides BWI76_RS17190 and BWI76_RS17195 are separated by 211 nucleotides BWI76_RS17195 and BWI76_RS17200 are separated by 58 nucleotides BWI76_RS17200 and BWI76_RS17205 are separated by 32 nucleotides
BWI76_RS17180: BWI76_RS17180 - hypothetical protein, at 3,561,034 to 3,561,318
_RS17180
BWI76_RS17185: BWI76_RS17185 - fructosamine kinase family protein, at 3,561,425 to 3,562,297
_RS17185
BWI76_RS17190: BWI76_RS17190 - TonB system transport protein TonB, at 3,562,317 to 3,563,054
_RS17190
BWI76_RS17195: BWI76_RS17195 - hypothetical protein, at 3,563,266 to 3,563,562
_RS17195
BWI76_RS17200: BWI76_RS17200 - hypothetical protein, at 3,563,621 to 3,563,905
_RS17200
BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398
_RS17205
Position (kb)
3562
3563
3564 Strain fitness (log2 ratio)
-2
-1
0
1 at 3561.767 kb on + strand, within BWI76_RS17185 at 3562.198 kb on + strand, within BWI76_RS17185 at 3562.548 kb on + strand, within BWI76_RS17190 at 3562.960 kb on - strand, within BWI76_RS17190 at 3562.965 kb on - strand, within BWI76_RS17190 at 3563.466 kb on + strand, within BWI76_RS17195 at 3563.639 kb on + strand at 3563.776 kb on - strand, within BWI76_RS17200 at 3563.964 kb on - strand at 3563.964 kb on - strand at 3563.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant remove 3,561,767 + BWI76_RS17185 0.39 -0.6 3,562,198 + BWI76_RS17185 0.89 +0.2 3,562,548 + BWI76_RS17190 0.31 -1.8 3,562,960 - BWI76_RS17190 0.87 -1.0 3,562,965 - BWI76_RS17190 0.88 -1.1 3,563,466 + BWI76_RS17195 0.67 -1.2 3,563,639 + -0.1 3,563,776 - BWI76_RS17200 0.54 +0.4 3,563,964 - -0.5 3,563,964 - +0.6 3,563,978 - -1.5
Or see this region's nucleotide sequence