Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14250

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotidesBWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotidesBWI76_RS14250 and BWI76_RS14255 overlap by 8 nucleotidesBWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotides BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694 _RS14240 BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251 _RS14245 BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781 _RS14250 BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778 _RS14255 BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930 _RS14260 Position (kb) 2980 2981 2982Strain fitness (log2 ratio) -2 -1 0 1 2at 2979.367 kb on + strandat 2979.468 kb on - strand, within BWI76_RS14240at 2979.563 kb on - strand, within BWI76_RS14240at 2979.626 kb on + strand, within BWI76_RS14240at 2979.634 kb on - strand, within BWI76_RS14240at 2979.743 kb on + strandat 2979.796 kb on + strand, within BWI76_RS14245at 2979.926 kb on + strand, within BWI76_RS14245at 2979.943 kb on - strand, within BWI76_RS14245at 2979.990 kb on - strand, within BWI76_RS14245at 2980.261 kb on + strandat 2980.330 kb on - strandat 2980.581 kb on + strandat 2980.589 kb on - strand, within BWI76_RS14250at 2980.703 kb on + strand, within BWI76_RS14250at 2980.711 kb on - strand, within BWI76_RS14250at 2980.778 kb on + strand, within BWI76_RS14250at 2980.851 kb on - strand, within BWI76_RS14250at 2980.870 kb on - strand, within BWI76_RS14250at 2980.872 kb on - strand, within BWI76_RS14250at 2980.875 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.918 kb on + strand, within BWI76_RS14250at 2980.926 kb on - strand, within BWI76_RS14250at 2980.935 kb on - strand, within BWI76_RS14250at 2981.037 kb on - strand, within BWI76_RS14250at 2981.158 kb on - strand, within BWI76_RS14250at 2981.255 kb on + strand, within BWI76_RS14250at 2981.255 kb on + strand, within BWI76_RS14250at 2981.263 kb on - strandat 2981.331 kb on - strand, within BWI76_RS14250at 2981.362 kb on - strand, within BWI76_RS14250at 2981.362 kb on - strand, within BWI76_RS14250at 2981.383 kb on + strand, within BWI76_RS14250at 2981.565 kb on - strand, within BWI76_RS14250at 2981.581 kb on - strand, within BWI76_RS14250at 2981.625 kb on + strand, within BWI76_RS14250at 2981.635 kb on + strand, within BWI76_RS14250at 2981.643 kb on - strandat 2981.697 kb on + strandat 2981.726 kb on + strandat 2981.736 kb on + strandat 2981.744 kb on - strandat 2981.824 kb on + strandat 2982.006 kb on + strand, within BWI76_RS14255at 2982.162 kb on + strand, within BWI76_RS14255at 2982.170 kb on - strand, within BWI76_RS14255at 2982.227 kb on - strand, within BWI76_RS14255at 2982.232 kb on - strand, within BWI76_RS14255at 2982.253 kb on + strand, within BWI76_RS14255at 2982.261 kb on - strand, within BWI76_RS14255at 2982.271 kb on + strand, within BWI76_RS14255at 2982.297 kb on + strand, within BWI76_RS14255at 2982.366 kb on - strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255at 2982.488 kb on + strand, within BWI76_RS14255

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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2,979,367 + -1.2
2,979,468 - BWI76_RS14240 0.30 +0.6
2,979,563 - BWI76_RS14240 0.59 +0.9
2,979,626 + BWI76_RS14240 0.79 -1.3
2,979,634 - BWI76_RS14240 0.81 -0.2
2,979,743 + -1.1
2,979,796 + BWI76_RS14245 0.13 +0.1
2,979,926 + BWI76_RS14245 0.38 -0.3
2,979,943 - BWI76_RS14245 0.41 +1.4
2,979,990 - BWI76_RS14245 0.50 +0.0
2,980,261 + -0.6
2,980,330 - -1.2
2,980,581 + +2.3
2,980,589 - BWI76_RS14250 0.16 -0.2
2,980,703 + BWI76_RS14250 0.24 +0.4
2,980,711 - BWI76_RS14250 0.25 -1.2
2,980,778 + BWI76_RS14250 0.29 +0.2
2,980,851 - BWI76_RS14250 0.34 +0.1
2,980,870 - BWI76_RS14250 0.36 -0.7
2,980,872 - BWI76_RS14250 0.36 -0.5
2,980,875 + BWI76_RS14250 0.36 -0.8
2,980,918 + BWI76_RS14250 0.39 -0.2
2,980,918 + BWI76_RS14250 0.39 +1.4
2,980,926 - BWI76_RS14250 0.40 -1.0
2,980,935 - BWI76_RS14250 0.40 +0.6
2,981,037 - BWI76_RS14250 0.47 +0.5
2,981,158 - BWI76_RS14250 0.56 -1.0
2,981,255 + BWI76_RS14250 0.63 -0.7
2,981,255 + BWI76_RS14250 0.63 -1.1
2,981,263 - +0.2
2,981,331 - BWI76_RS14250 0.68 +0.1
2,981,362 - BWI76_RS14250 0.70 -0.1
2,981,362 - BWI76_RS14250 0.70 -0.6
2,981,383 + BWI76_RS14250 0.72 -0.2
2,981,565 - BWI76_RS14250 0.85 -0.2
2,981,581 - BWI76_RS14250 0.86 +0.2
2,981,625 + BWI76_RS14250 0.89 -0.1
2,981,635 + BWI76_RS14250 0.90 -0.0
2,981,643 - +1.0
2,981,697 + -1.1
2,981,726 + -0.2
2,981,736 + +0.4
2,981,744 - -0.6
2,981,824 + -0.1
2,982,006 + BWI76_RS14255 0.23 -0.1
2,982,162 + BWI76_RS14255 0.39 +0.1
2,982,170 - BWI76_RS14255 0.39 -0.4
2,982,227 - BWI76_RS14255 0.45 -0.8
2,982,232 - BWI76_RS14255 0.46 -1.8
2,982,253 + BWI76_RS14255 0.48 +0.6
2,982,261 - BWI76_RS14255 0.48 -0.8
2,982,271 + BWI76_RS14255 0.49 +0.5
2,982,297 + BWI76_RS14255 0.52 +0.1
2,982,366 - BWI76_RS14255 0.59 +0.3
2,982,488 + BWI76_RS14255 0.71 -0.8
2,982,488 + BWI76_RS14255 0.71 +0.1

Or see this region's nucleotide sequence