Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13540 and BWI76_RS13545 are separated by 11 nucleotides BWI76_RS13545 and BWI76_RS13550 are separated by 9 nucleotides BWI76_RS13550 and BWI76_RS13555 overlap by 17 nucleotides BWI76_RS13555 and BWI76_RS13560 overlap by 4 nucleotides
BWI76_RS13540: BWI76_RS13540 - ABC transporter substrate-binding protein, at 2,823,528 to 2,824,469
_RS13540
BWI76_RS13545: BWI76_RS13545 - N-acetyltransferase, at 2,824,481 to 2,824,990
_RS13545
BWI76_RS13550: BWI76_RS13550 - polar amino acid ABC transporter inner membrane subunit, at 2,825,000 to 2,825,926
_RS13550
BWI76_RS13555: BWI76_RS13555 - ABC transporter, at 2,825,910 to 2,826,689
_RS13555
BWI76_RS13560: BWI76_RS13560 - amino acid ABC transporter substrate-binding subunit, at 2,826,686 to 2,827,579
_RS13560
Position (kb)
2824
2825
2826 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2824.032 kb on + strand, within BWI76_RS13540 at 2824.100 kb on - strand, within BWI76_RS13540 at 2824.167 kb on + strand, within BWI76_RS13540 at 2824.175 kb on - strand, within BWI76_RS13540 at 2824.910 kb on + strand, within BWI76_RS13545 at 2825.210 kb on + strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.538 kb on - strand, within BWI76_RS13550 at 2825.652 kb on - strand, within BWI76_RS13550 at 2825.689 kb on + strand, within BWI76_RS13550 at 2825.697 kb on - strand, within BWI76_RS13550 at 2825.716 kb on - strand, within BWI76_RS13550 at 2825.791 kb on + strand, within BWI76_RS13550 at 2825.902 kb on - strand at 2825.906 kb on + strand at 2826.466 kb on - strand, within BWI76_RS13555
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 2,824,032 + BWI76_RS13540 0.54 +0.9 2,824,100 - BWI76_RS13540 0.61 -1.5 2,824,167 + BWI76_RS13540 0.68 +0.1 2,824,175 - BWI76_RS13540 0.69 -0.3 2,824,910 + BWI76_RS13545 0.84 -0.5 2,825,210 + BWI76_RS13550 0.23 -0.1 2,825,514 - BWI76_RS13550 0.55 +0.3 2,825,514 - BWI76_RS13550 0.55 +0.1 2,825,538 - BWI76_RS13550 0.58 -0.1 2,825,652 - BWI76_RS13550 0.70 -1.3 2,825,689 + BWI76_RS13550 0.74 +0.4 2,825,697 - BWI76_RS13550 0.75 +4.5 2,825,716 - BWI76_RS13550 0.77 -0.7 2,825,791 + BWI76_RS13550 0.85 -0.0 2,825,902 - -0.1 2,825,906 + -0.3 2,826,466 - BWI76_RS13555 0.71 -1.2
Or see this region's nucleotide sequence