Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20975

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotidesBWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotidesBWI76_RS20975 and BWI76_RS20980 are separated by 422 nucleotides BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407 _RS20965 BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045 _RS20970 BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447 _RS20975 BWI76_RS20980: BWI76_RS20980 - MFS transporter, at 4,385,870 to 4,387,324 _RS20980 Position (kb) 4384 4385 4386Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4383.163 kb on - strand, within BWI76_RS20965at 4383.337 kb on - strandat 4383.346 kb on - strandat 4383.379 kb on + strandat 4383.431 kb on + strandat 4383.859 kb on + strand, within BWI76_RS20970at 4384.255 kb on + strand, within BWI76_RS20975at 4384.435 kb on - strand, within BWI76_RS20975at 4384.529 kb on - strand, within BWI76_RS20975at 4384.759 kb on - strand, within BWI76_RS20975at 4384.787 kb on - strand, within BWI76_RS20975at 4384.884 kb on + strand, within BWI76_RS20975at 4384.922 kb on - strand, within BWI76_RS20975at 4384.923 kb on - strand, within BWI76_RS20975at 4384.953 kb on - strand, within BWI76_RS20975at 4384.978 kb on + strand, within BWI76_RS20975at 4384.983 kb on + strand, within BWI76_RS20975at 4385.068 kb on - strand, within BWI76_RS20975at 4385.205 kb on + strand, within BWI76_RS20975at 4385.213 kb on - strand, within BWI76_RS20975at 4385.213 kb on - strand, within BWI76_RS20975at 4385.231 kb on - strand, within BWI76_RS20975at 4385.407 kb on + strandat 4385.408 kb on - strandat 4385.512 kb on + strandat 4385.515 kb on - strandat 4385.522 kb on + strandat 4385.651 kb on + strandat 4385.717 kb on + strandat 4385.717 kb on + strandat 4385.717 kb on + strandat 4385.764 kb on + strandat 4385.785 kb on - strandat 4385.820 kb on + strandat 4385.863 kb on - strandat 4385.966 kb on + strandat 4386.027 kb on - strand, within BWI76_RS20980at 4386.104 kb on + strand, within BWI76_RS20980at 4386.104 kb on + strand, within BWI76_RS20980at 4386.104 kb on + strand, within BWI76_RS20980at 4386.104 kb on + strand, within BWI76_RS20980at 4386.104 kb on + strand, within BWI76_RS20980at 4386.107 kb on - strand, within BWI76_RS20980at 4386.112 kb on - strand, within BWI76_RS20980at 4386.117 kb on - strand, within BWI76_RS20980at 4386.143 kb on - strand, within BWI76_RS20980at 4386.187 kb on - strand, within BWI76_RS20980at 4386.195 kb on + strand, within BWI76_RS20980at 4386.195 kb on + strand, within BWI76_RS20980at 4386.195 kb on + strand, within BWI76_RS20980at 4386.203 kb on - strand, within BWI76_RS20980at 4386.203 kb on - strand, within BWI76_RS20980at 4386.203 kb on - strand, within BWI76_RS20980at 4386.272 kb on - strand, within BWI76_RS20980at 4386.369 kb on - strand, within BWI76_RS20980at 4386.374 kb on + strand, within BWI76_RS20980at 4386.393 kb on - strand, within BWI76_RS20980at 4386.408 kb on + strand, within BWI76_RS20980

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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4,383,163 - BWI76_RS20965 0.76 -0.8
4,383,337 - +1.2
4,383,346 - -0.7
4,383,379 + -0.3
4,383,431 + -0.8
4,383,859 + BWI76_RS20970 0.71 +1.8
4,384,255 + BWI76_RS20975 0.12 -1.1
4,384,435 - BWI76_RS20975 0.25 -1.8
4,384,529 - BWI76_RS20975 0.32 -0.1
4,384,759 - BWI76_RS20975 0.49 +0.5
4,384,787 - BWI76_RS20975 0.51 -0.3
4,384,884 + BWI76_RS20975 0.58 +0.1
4,384,922 - BWI76_RS20975 0.61 +0.6
4,384,923 - BWI76_RS20975 0.61 +3.0
4,384,953 - BWI76_RS20975 0.64 -0.2
4,384,978 + BWI76_RS20975 0.65 -0.2
4,384,983 + BWI76_RS20975 0.66 -1.9
4,385,068 - BWI76_RS20975 0.72 -1.1
4,385,205 + BWI76_RS20975 0.82 +0.5
4,385,213 - BWI76_RS20975 0.83 +0.1
4,385,213 - BWI76_RS20975 0.83 +1.3
4,385,231 - BWI76_RS20975 0.84 -0.1
4,385,407 + +0.5
4,385,408 - -0.7
4,385,512 + -0.2
4,385,515 - -3.6
4,385,522 + +1.1
4,385,651 + +0.3
4,385,717 + -1.2
4,385,717 + +1.3
4,385,717 + +0.7
4,385,764 + -0.4
4,385,785 - -0.1
4,385,820 + -0.2
4,385,863 - -0.3
4,385,966 + +0.2
4,386,027 - BWI76_RS20980 0.11 -0.7
4,386,104 + BWI76_RS20980 0.16 -0.7
4,386,104 + BWI76_RS20980 0.16 -2.2
4,386,104 + BWI76_RS20980 0.16 +0.2
4,386,104 + BWI76_RS20980 0.16 +1.4
4,386,104 + BWI76_RS20980 0.16 -0.2
4,386,107 - BWI76_RS20980 0.16 +0.3
4,386,112 - BWI76_RS20980 0.17 -1.2
4,386,117 - BWI76_RS20980 0.17 -0.5
4,386,143 - BWI76_RS20980 0.19 -1.3
4,386,187 - BWI76_RS20980 0.22 +0.2
4,386,195 + BWI76_RS20980 0.22 +0.8
4,386,195 + BWI76_RS20980 0.22 +0.5
4,386,195 + BWI76_RS20980 0.22 +1.0
4,386,203 - BWI76_RS20980 0.23 +1.5
4,386,203 - BWI76_RS20980 0.23 +0.6
4,386,203 - BWI76_RS20980 0.23 +0.9
4,386,272 - BWI76_RS20980 0.28 -0.6
4,386,369 - BWI76_RS20980 0.34 -1.2
4,386,374 + BWI76_RS20980 0.35 -0.9
4,386,393 - BWI76_RS20980 0.36 -0.5
4,386,408 + BWI76_RS20980 0.37 +0.8

Or see this region's nucleotide sequence