Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13225

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13215 and BWI76_RS13220 are separated by 243 nucleotidesBWI76_RS13220 and BWI76_RS13225 are separated by 86 nucleotidesBWI76_RS13225 and BWI76_RS13230 are separated by 132 nucleotides BWI76_RS13215: BWI76_RS13215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,758,236 to 2,759,234 _RS13215 BWI76_RS13220: BWI76_RS13220 - N-acetyltransferase, at 2,759,478 to 2,759,972 _RS13220 BWI76_RS13225: BWI76_RS13225 - hypothetical protein, at 2,760,059 to 2,760,985 _RS13225 BWI76_RS13230: BWI76_RS13230 - GGDEF domain-containing protein, at 2,761,118 to 2,762,089 _RS13230 Position (kb) 2760 2761Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 2759.150 kb on + strandat 2759.150 kb on + strandat 2759.153 kb on + strandat 2759.158 kb on - strandat 2759.204 kb on + strandat 2759.454 kb on + strandat 2759.524 kb on - strandat 2759.681 kb on - strand, within BWI76_RS13220at 2759.835 kb on + strand, within BWI76_RS13220at 2760.021 kb on - strandat 2760.034 kb on - strandat 2760.103 kb on - strandat 2760.287 kb on + strand, within BWI76_RS13225at 2760.622 kb on + strand, within BWI76_RS13225at 2760.635 kb on - strand, within BWI76_RS13225at 2760.796 kb on + strand, within BWI76_RS13225at 2760.927 kb on + strandat 2761.278 kb on + strand, within BWI76_RS13230at 2761.776 kb on - strand, within BWI76_RS13230

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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2,759,150 + +1.7
2,759,150 + -1.0
2,759,153 + +0.2
2,759,158 - -0.5
2,759,204 + -0.3
2,759,454 + -1.8
2,759,524 - -0.8
2,759,681 - BWI76_RS13220 0.41 +1.6
2,759,835 + BWI76_RS13220 0.72 -0.8
2,760,021 - -0.8
2,760,034 - -0.5
2,760,103 - -0.8
2,760,287 + BWI76_RS13225 0.25 -0.0
2,760,622 + BWI76_RS13225 0.61 +0.1
2,760,635 - BWI76_RS13225 0.62 -0.1
2,760,796 + BWI76_RS13225 0.80 +5.1
2,760,927 + -0.3
2,761,278 + BWI76_RS13230 0.16 +0.7
2,761,776 - BWI76_RS13230 0.68 -0.5

Or see this region's nucleotide sequence