Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08760

Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08755 and BWI76_RS08760 are separated by 85 nucleotidesBWI76_RS08760 and BWI76_RS08765 overlap by 4 nucleotides BWI76_RS08755: BWI76_RS08755 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,845,038 to 1,846,327 _RS08755 BWI76_RS08760: BWI76_RS08760 - biotin synthase, at 1,846,413 to 1,847,453 _RS08760 BWI76_RS08765: BWI76_RS08765 - 8-amino-7-oxononanoate synthase, at 1,847,450 to 1,848,607 _RS08765 Position (kb) 1846 1847 1848Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 1845.557 kb on + strand, within BWI76_RS08755at 1845.732 kb on + strand, within BWI76_RS08755at 1845.852 kb on - strand, within BWI76_RS08755at 1845.936 kb on + strand, within BWI76_RS08755at 1846.147 kb on + strand, within BWI76_RS08755at 1846.183 kb on - strand, within BWI76_RS08755at 1846.408 kb on + strandat 1846.536 kb on - strand, within BWI76_RS08760at 1846.653 kb on + strand, within BWI76_RS08760at 1846.747 kb on + strand, within BWI76_RS08760at 1846.872 kb on + strand, within BWI76_RS08760at 1846.937 kb on - strand, within BWI76_RS08760at 1847.691 kb on + strand, within BWI76_RS08765at 1847.826 kb on - strand, within BWI76_RS08765at 1848.089 kb on + strand, within BWI76_RS08765

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
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1,845,557 + BWI76_RS08755 0.40 +0.7
1,845,732 + BWI76_RS08755 0.54 -2.1
1,845,852 - BWI76_RS08755 0.63 +1.3
1,845,936 + BWI76_RS08755 0.70 -0.9
1,846,147 + BWI76_RS08755 0.86 -0.6
1,846,183 - BWI76_RS08755 0.89 -0.7
1,846,408 + +0.1
1,846,536 - BWI76_RS08760 0.12 -0.3
1,846,653 + BWI76_RS08760 0.23 -0.8
1,846,747 + BWI76_RS08760 0.32 +1.2
1,846,872 + BWI76_RS08760 0.44 +6.3
1,846,937 - BWI76_RS08760 0.50 -0.0
1,847,691 + BWI76_RS08765 0.21 +0.2
1,847,826 - BWI76_RS08765 0.32 +0.1
1,848,089 + BWI76_RS08765 0.55 -2.4

Or see this region's nucleotide sequence