Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05770 and BWI76_RS05775 are separated by 11 nucleotides BWI76_RS05775 and BWI76_RS05780 overlap by 4 nucleotides BWI76_RS05780 and BWI76_RS05785 overlap by 4 nucleotides
BWI76_RS05770: BWI76_RS05770 - nucleoside triphosphatase, D5 family protein, at 1,214,563 to 1,216,896
_RS05770
BWI76_RS05775: BWI76_RS05775 - hypothetical protein, at 1,216,908 to 1,217,228
_RS05775
BWI76_RS05780: BWI76_RS05780 - hypothetical protein, at 1,217,225 to 1,217,452
_RS05780
BWI76_RS05785: BWI76_RS05785 - CI repressor, at 1,217,449 to 1,218,000
_RS05785
Position (kb)
1214
1215
1216
1217 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1213.653 kb on + strand at 1213.667 kb on + strand at 1213.669 kb on + strand at 1213.669 kb on + strand at 1213.677 kb on - strand at 1213.678 kb on - strand at 1213.687 kb on + strand at 1213.687 kb on + strand at 1213.692 kb on + strand at 1213.695 kb on - strand at 1213.695 kb on - strand at 1213.695 kb on - strand at 1213.700 kb on - strand at 1213.702 kb on + strand at 1213.702 kb on + strand at 1213.709 kb on + strand at 1213.710 kb on - strand at 1213.710 kb on - strand at 1213.710 kb on - strand at 1213.714 kb on + strand at 1213.716 kb on - strand at 1213.717 kb on - strand at 1213.777 kb on + strand at 1213.785 kb on - strand at 1213.801 kb on + strand at 1213.804 kb on - strand at 1213.883 kb on - strand at 1213.912 kb on + strand at 1213.920 kb on - strand at 1213.936 kb on + strand at 1213.944 kb on - strand at 1213.960 kb on + strand at 1213.960 kb on + strand at 1213.968 kb on - strand at 1213.974 kb on + strand at 1214.165 kb on + strand at 1214.165 kb on + strand at 1214.167 kb on + strand at 1214.167 kb on + strand at 1214.175 kb on - strand at 1214.593 kb on - strand at 1214.700 kb on + strand at 1214.735 kb on - strand at 1215.126 kb on + strand, within BWI76_RS05770 at 1215.300 kb on + strand, within BWI76_RS05770 at 1215.509 kb on + strand, within BWI76_RS05770 at 1215.604 kb on - strand, within BWI76_RS05770 at 1215.717 kb on + strand, within BWI76_RS05770 at 1216.074 kb on - strand, within BWI76_RS05770 at 1216.093 kb on - strand, within BWI76_RS05770 at 1216.095 kb on + strand, within BWI76_RS05770 at 1216.245 kb on + strand, within BWI76_RS05770 at 1216.262 kb on + strand, within BWI76_RS05770 at 1216.580 kb on + strand, within BWI76_RS05770 at 1216.703 kb on - strand at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.190 kb on - strand, within BWI76_RS05775 at 1217.692 kb on - strand, within BWI76_RS05785 at 1217.760 kb on - strand, within BWI76_RS05785 at 1217.812 kb on - strand, within BWI76_RS05785
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 1,213,653 + -1.1 1,213,667 + +0.0 1,213,669 + -0.6 1,213,669 + -0.4 1,213,677 - -0.6 1,213,678 - +0.9 1,213,687 + -1.3 1,213,687 + -0.3 1,213,692 + -1.5 1,213,695 - -1.3 1,213,695 - -0.1 1,213,695 - -0.8 1,213,700 - +1.1 1,213,702 + -1.1 1,213,702 + -0.4 1,213,709 + -1.1 1,213,710 - -2.1 1,213,710 - -0.9 1,213,710 - +0.1 1,213,714 + +1.3 1,213,716 - -1.3 1,213,717 - +0.3 1,213,777 + +0.7 1,213,785 - -1.9 1,213,801 + -2.3 1,213,804 - +0.3 1,213,883 - -0.0 1,213,912 + +0.1 1,213,920 - -0.2 1,213,936 + -0.7 1,213,944 - -0.6 1,213,960 + +0.8 1,213,960 + +1.7 1,213,968 - +1.1 1,213,974 + -0.7 1,214,165 + -1.1 1,214,165 + +0.2 1,214,167 + +0.5 1,214,167 + -0.5 1,214,175 - +0.6 1,214,593 - +1.1 1,214,700 + +0.7 1,214,735 - -0.6 1,215,126 + BWI76_RS05770 0.24 -0.3 1,215,300 + BWI76_RS05770 0.32 +0.3 1,215,509 + BWI76_RS05770 0.41 -0.3 1,215,604 - BWI76_RS05770 0.45 +6.1 1,215,717 + BWI76_RS05770 0.49 -1.2 1,216,074 - BWI76_RS05770 0.65 +0.3 1,216,093 - BWI76_RS05770 0.66 -0.8 1,216,095 + BWI76_RS05770 0.66 +0.4 1,216,245 + BWI76_RS05770 0.72 +0.8 1,216,262 + BWI76_RS05770 0.73 +0.2 1,216,580 + BWI76_RS05770 0.86 +0.3 1,216,703 - -1.8 1,217,187 + BWI76_RS05775 0.87 +0.8 1,217,187 + BWI76_RS05775 0.87 +0.8 1,217,190 - BWI76_RS05775 0.88 -1.4 1,217,692 - BWI76_RS05785 0.44 -0.8 1,217,760 - BWI76_RS05785 0.56 +0.1 1,217,812 - BWI76_RS05785 0.66 -0.7
Or see this region's nucleotide sequence